LOCUS       Exported                3031 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.a3-7wnb8up
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 96 documents from 
            Done.gb Level 1 Backbone, Seq 4 - Seq 5. Amp Resistance. RFP for R/W
            Screening(from pL1 [AmpR, RFP, S4-S5]). ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(3..8)
                     /label=BBb scar
     misc_feature    89..94
                     /label=BBb scar
     misc_feature    complement(101..106)
                     /label=BBb scar
     CDS             174..1031
                     /label=AmpR
     primer_bind     862..882
                     /label=JH1030F
     misc_feature    869..874
                     /label=BBb scar
     misc_feature    complement(886..891)
                     /label=BBb scar
     misc_feature    1178..1222
                     /label=pL1 S4
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    1181..1186
                     /label=EcoRI
     primer_bind     1223..1226
                     /label=RJ_00069Fa
     misc_feature    1227..1317
                     /label=AmpR Promoter
                     /note="Geneious type: promoter_prokaryotic"
                     /note="/Transferred_From=pFUGW"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pFUGW extraction"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1318..1331
                     /label=RBS
                     /label=nonstandard type:  motif
                     /note="/created_by=User"
     CDS             1332..2192
                     /codon_start=1
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(1447..1452)
                     /label=ApaLI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     protein_bind    2047..2052
                     /label=BsaI Bind
                     /note="/created_by=User"
     misc_feature    2047..2052
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     misc_feature    2193..2196
                     /label=mMoClo
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    complement(2197..2241)
                     /label=SeqX
                     /label=nonstandard type:  motif
     misc_feature    2242..2249
                     /label=PAC
     rep_origin      2288..2397
                     /label=RK2 trfa
     misc_feature    complement(2394..2413)
                     /label=JH1030R
     misc_feature    2402..2407
                     /label=BBb scar
     rep_origin      complement(join(2425..3031,1..76))
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    3023..3028
                     /label=BBb scar
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaagg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcacg
     1141 aattggccag cgctgcatta ccctgttatc cctaaccggt gaattccctt atgtgagtgt
     1201 aaaaggcagg cgagtttgtc ccggagcgcg gaacccctat ttgtttattt ttctaaatac
     1261 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1321 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1381 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1441 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1501 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1561 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1621 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1681 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1741 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1801 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1861 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1921 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1981 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2041 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2101 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2161 agataggtgc ctcactgatt aagcattggt aacgctggta tcactagtat tacagaggta
     2221 agttataaca gtcgcctaac cttaattaat agggataaca gggtaatatc ttgccctgca
     2281 cgaataccag cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac
     2341 acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct
     2401 aggatctgcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3001 gcagattacg cgcagaaaaa aaggatctca a
//
