LOCUS       Exported                3136 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     25..47
                     /label=KK_lvl1b_F66
     misc_feature    36..39
                     /label=Ins 7
                     /label=nonstandard type:  Gg_overhang
     primer_bind     51..80
                     /label=KK_lvl1_f60
     misc_feature    complement(53..70)
                     /label=I-SceI
     primer_bind     53..66
                     /label=KK_lvl1_r61
     misc_feature    74..118
                     /label=pL1 S1
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    complement(94..118)
                     /label=RJ_00429Ra
     protein_bind    119..125
                     /label=SapI Bind
     misc_feature    119..125
                     /label=SapI
     misc_feature    127..129
                     /label=pL1 T1
                     /label=nonstandard type:  Gg_overhang
     misc_feature    134..703
                     /label=2x cHS4 Insulator
                     /label=nonstandard type:  Insulator
     misc_feature    158..404
                     /label=cHS4 Core
                     /label=nonstandard type:  Insulator
     protein_bind    212..216
                     /label=CTCF Bind (Short)
     misc_feature    437..683
                     /label=cHS4 Core
                     /label=nonstandard type:  Insulator
     protein_bind    491..495
                     /label=CTCF Bind (Short)
     primer_bind     684..706
                     /label=KK_CHS4_SeqPrimer
     misc_feature    708..1023
                     /label=U6/TetO
     misc_feature    709..1182
                     /label=TTGTCTTCGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCA
                     /label=TTGTCTTCGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAG
                     CGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTAT
                     GCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACA
                     GCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCG
                     GGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG
                     GCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGC
                     GAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCG
                     CCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCA
                     CTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACA
                     CCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGT
                     GACGAAGACAA
     misc_feature    976..997
                     /label=TetO site
     ncRNA           1024..1182
                     /label=transcript
     misc_feature    1024..1072
                     /label=5PrimeEnd
     misc_feature    1024..1027
                     /label=Overlap
     ncRNA           1073..1094
                     /label=FF4
     misc_feature    1095..1111
                     /label=Loop
     ncRNA           1112..1133
                     /label=FF4_as
     misc_feature    1134..1182
                     /label=3PrimeEnd
     misc_feature    1187..1189
                     /label=pL1 T2
                     /label=nonstandard type:  Gg_overhang
     misc_feature    complement(1191..1197)
                     /label=SapI
     protein_bind    complement(1191..1197)
                     /label=SapI Bind
     misc_feature    1198..1242
                     /label=pL1 S2
                     /label=nonstandard type:  Gibson_Overhang
     primer_bind     1198..1230
                     /label=RJ_00429Fa
     misc_feature    1251..1268
                     /label=I-SceI
     primer_bind     complement(1255..1288)
                     /label=KK_lvl1_r61
     misc_feature    1279..1282
                     /label=Ins 1
                     /label=nonstandard type:  Gg_overhang
     rep_origin      1289..1398
                     /label=RK2 trfa
     misc_feature    complement(1395..1414)
                     /label=JH1030R
     rep_origin      1426..2108
                     /label=ColE1 origin
     primer_bind     complement(1461..1480)
                     /label=KK_SeqR_SeqPrimer
     misc_feature    1697..1702
                     /label=ApaLI
     misc_feature    1794..1802
                     /label=AlwNI
     CDS             complement(2203..3063)
                     /label=AmpR
     primer_bind     2894..2914
                     /label=JH1030F
     misc_feature    2943..2948
                     /label=ApaLI
     misc_feature    complement(3064..3077)
                     /label=RBS
                     /label=nonstandard type:  motif
     primer_bind     3098..3118
                     /label=JH856R
     misc_feature    complement(3105..3133)
                     /label=pAmp
ORIGIN
        1 atttagaaaa ataaacaaat aggggttccg cgcacgaatt ggccagcgct gcattaccct
       61 gttatcccta accggtttac cgagctctta ttggttttca aacttcattg actgtgccgc
      121 tcttcaaccg gagttctgca attcatgtga tctgcgcctg ggagctcacg gggacagccc
      181 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
      241 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
      301 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
      361 ctctttgagc ctgcagacac ctggggggat acggggaaaa agctaggctg aaagaggatc
      421 ttagaatgac acgcgcctgg gagctcacgg ggacagcccc cccccaaagc ccccagggat
      481 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
      541 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
      601 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
      661 tggggggata cggggaaaaa gctggatcat aactcgacgt gcatacttag gcaaaacgca
      721 ccacgtgacg gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc
      781 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
      841 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      901 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      961 ccgtaacttg aaagtactct atcattgata gagttatata tcttgtggaa aggacgaaac
     1021 accgtgctcg cttcggcagc acatatacta caccctaatg atactctctt acgcttgaag
     1081 tatttaatta aatagtgaag ccacagatgt atttaattaa agacttcaag cggaagcgac
     1141 tatcccgtcg cttctgcaaa gcggacttcg gtccgctttt ttcgctcgct gaagagcggt
     1201 gcgtttttat gcttgtagta ttgtataatg tttttaagat ccttaattaa tagggataac
     1261 agggtaatat cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg
     1321 cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc
     1381 cggcatcgtt gcgccacatc taggatctgc caggaaccgt aaaaaggccg cgttgctggc
     1441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2341 gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3061 catactcttc ctttttcaat attattgaag catttatcaa ggttattgtc tcatgagcgg
     3121 atacatattt gaatgt
//
