LOCUS       Exported                5026 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.jones.va-bkutpai
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pBD24.XcRc.gb 
            [STX-1] KanR-pUC-PB 3'ITR-cHS4--cHS4-PB 5'ITR.
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          652..765
                     /label=Invitrogen vector 722
                     /note="invitrogen: """"722"
     source          771..780
                     /label=source synthetic DNA construct
                     /note="organism: synthetic DNA construct mol_type: other 
                     DNA"
     source          781..1085
                     /label=source synthetic DNA construct
                     /note="organism: synthetic DNA construct mol_type: other 
                     DNA"
     source          3468..3835
                     /label=source synthetic DNA construct
                     /note="organism: synthetic DNA construct mol_type: other 
                     DNA"
     source          3836..3857
                     /label=source synthetic DNA construct
                     /note="organism: synthetic DNA construct mol_type: other 
                     DNA"
     source          3957..4996
                     /label=Invitrogen vector 722
                     /note="invitrogen: """"722"
     source          join(4997..5026,1..651)
                     /label=Invitrogen vector 722
                     /note="invitrogen: """"722"
     misc_feature    331..336
                     /label=ApaLI
                     /note="Recognition_pattern: G^TGCAC Methylation: 
                     CpG:blocked, EcoKI:variable annotation_group: GTGCAC note: 
                     Geneious type: restriction site"
     primer_bind     553..572
                     /label=BP0148
                     /note="created_by: Ross Sequence: TTTGTGATGCTCGTCAGGGG"
     primer_bind     689..708
                     /label=G00101
                     /note="Mismatches: 0 created_by: primer3 annotation_group: 
                     612499489552558e4dee7-b262-4abe-b962-04f8f58b1efe %GC: 50.0
                     Tm: 58.3 Hairpin_Tm: None Self_Dimer_Tm: None Sequence: 
                     ATTACCGCCTTTGAGTGAGC"
     misc_feature    780..781
                     /label=origin
                     /label=nonstandard type:  origin
     misc_feature    787..790
                     /label=TTAA
                     /label=nonstandard type:  motif
                     /note="created_by: jones"
     LTR             complement(791..1028)
                     /label=PB 3' ITR
                     /note="created_by: jones"
     primer_bind     complement(1055..1085)
                     /label=RJ_00517Rb
                     /note="Mismatches: 0 Extension: CAGATGGTCTCT %GC: 25.8 
                     Hairpin_Tm: 43.1 Self_Dimer_Tm: None Tm: 57.9 
                     annotation_group: 
                     1915985834817400749f1888-fc7d-4b32-89a1-582b7ddc29ec 
                     Sequence: CAACATGACTGTTTTTAAAGTACAAAATAAG created_by: 
                     primer3"
     misc_feature    1086..1655
                     /label=2x cHS4 Insulator
                     /label=nonstandard type:  Insulator
                     /note="created_by: User modified_by: User"
     primer_bind     1086..1108
                     /label=RJ_00516Fb
                     /note="Mismatches: 0 Extension: CAGATGGTCTCTTCGC %GC: 43.5 
                     Hairpin_Tm: 35.8 Self_Dimer_Tm: 10.4 Tm: 60.2 
                     annotation_group: 
                     1915985101000600c833af6c-17a2-43da-8e4a-34c464cff1c0 
                     Sequence: TTCTGCAATTCATGTGATCTGCG created_by: primer3"
     primer_bind     1086..1108
                     /label=RJ_00516Fa
                     /note="Mismatches: 0 Extension: CAGATGGTCTCTGTTG %GC: 43.5 
                     Hairpin_Tm: 35.8 Self_Dimer_Tm: 10.4 Tm: 60.2 
                     annotation_group: 
                     19159849768848005c5a7e0c-82ce-40e6-905b-66496d4ad282 
                     Sequence: TTCTGCAATTCATGTGATCTGCG created_by: primer3"
     misc_feature    1110..1356
                     /label=cHS4 Core
                     /label=nonstandard type:  Insulator
                     /note="created_by: User"
     protein_bind    1164..1168
                     /label=CTCF Bind (Short)
                     /note="created_by: User"
     misc_feature    1389..1635
                     /label=cHS4 Core
                     /label=nonstandard type:  Insulator
                     /note="created_by: User"
     protein_bind    1443..1447
                     /label=CTCF Bind (Short)
                     /note="created_by: User"
     primer_bind     complement(1634..1655)
                     /label=RJ_00516Rb
                     /note="Mismatches: 0 Extension: CAGATGGTCTCCTG %GC: 50.0 
                     Hairpin_Tm: None Self_Dimer_Tm: None Tm: 60.5 
                     annotation_group: 
                     1915985352598400e066bef0-3d9b-4af4-bebf-f63d1371c60f 
                     Sequence: TGCACGTCGAGTTATGATCCAG created_by: primer3"
     primer_bind     complement(1634..1655)
                     /label=RJ_00516Ra
                     /note="Mismatches: 0 Extension: CAGATGGTCTCTAC %GC: 50.0 
                     Hairpin_Tm: None Self_Dimer_Tm: None Tm: 60.5 
                     annotation_group: 
                     19159852230094003352887b-80e8-46f6-a761-bffc946c6376 
                     Sequence: TGCACGTCGAGTTATGATCCAG created_by: primer3"
     primer_bind     1656..1673
                     /label=RJ_00517Fa
                     /note="Mismatches: 0 Extension: CAGATGGTCTCGCA %GC: 61.1 
                     Hairpin_Tm: None Self_Dimer_Tm: 1.3 Tm: 59.1 
                     annotation_group: 
                     191598553836460040b6f362-ea63-4eb1-9ac5-3da5ec40ec1b 
                     Sequence: GTAAAACGACGGCCAGGC created_by: primer3"
     primer_bind     1656..1671
                     /label=M13F
                     /note="Sequence: GTAAAACGACGGCCAG Hairpin_Tm: None Tm: 52.4
                     Self_Dimer_Tm: None %GC: 56.3 created_by: primer3"
     misc_feature    1677..1679
                     /label=pL1 T1
                     /label=nonstandard type:  gg_overhang
                     /note="created_by: User"
     protein_bind    complement(1681..1687)
                     /label=SapI Bind
                     /note="created_by: User"
     misc_feature    1706..1750
                     /label=pL1 S1
                     /label=nonstandard type:  Gibson_Overhang
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0 
                     modified_by: User"
     primer_bind     1706..1750
                     /label=KK_seq1_F72
     primer_bind     1706..1730
                     /label=KK_Gseq1_F65
     primer_bind     complement(1719..1776)
                     /label=KK_Gseq1_R65
     protein_bind    1759..1776
                     /label=I-SceI Bind
                     /note="created_by: User"
     misc_feature    1777..1796
                     /label=New Feature
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1796..1836
                     /label=Term rrn
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1860..1933
                     /label=pTet
                     /label=pTet; PLtet prom
                     /note="created_by: User Imported_from: PlasMapper 
                     Transferred_From: pTet, PLtet prom Transferred_Similarity: 
                     100.00% -> 82.43% modified_by: User note: Geneious type: 
                     Promoter_prokaryotic"
     misc_feature    1860..1903
                     /label=tetO reg
                     /note="Imported_from: PlasMapper Transferred_From: tetO reg
                     Transferred_Similarity: 88.64% note: Geneious type: 
                     regulatory sequence"
     CDS             1940..2617
                     /label=mRFP
                     /note="created_by: User Transferred_From: mRFP 
                     Transferred_Similarity: 100.00%"
     misc_feature    1943..2614
                     /label=DsRed2 reporter
                     /label=nonstandard type:  domain
                     /note="Imported_from: PlasMapper Transferred_From: DsRed2 
                     reporter Transferred_Similarity: 74.93% modified_by: jones"
     misc_feature    complement(2171..2190)
                     /label=BP0023
                     /note="created_by: Ross Sequence: TTCAGGTAGTCCGGGATGTC 
                     note: Geneious type: primer_bind_reverse"
     terminator      2665..2708
                     /label=T1 term
                     /label=T1 term; rrnB_T1 term
                     /note="Imported_from: PlasMapper created_by: User 
                     Transferred_From: T1 term, rrnB_T1 term 
                     Transferred_Similarity: 100.00% -> 97.73%"
     terminator      2749..2768
                     /label=stem_loop_early_T7 term
                     /note="Imported_from: PlasMapper Transferred_From: 
                     stem_loop_early_T7 term Transferred_Similarity: 100.00%"
     misc_feature    2793..2796
                     /label=Ligation
                     /label=nonstandard type:  ligation
                     /note="created_by: jones"
     primer_bind     2797..2854
                     /label=KK_GseqX_F65
     protein_bind    complement(2797..2814)
                     /label=I-SceI Bind
                     /note="created_by: User"
     primer_bind     2817..2862
                     /label=KK_seqX_F71
     misc_feature    2818..2862
                     /label=pL1 SX
                     /label=nonstandard type:  Gibson_Overhang
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0 
                     modified_by: jones"
     primer_bind     complement(2879..2897)
                     /label=RJ_00517Ra
                     /note="Mismatches: 0 Extension: CAGATGGTCTCA %GC: 57.9 
                     Hairpin_Tm: 46.1 Self_Dimer_Tm: 23.9 Tm: 59.6 
                     annotation_group: 
                     1915985706626300ecae016f-8be0-485c-b920-82b1e927ab3c 
                     Sequence: GCGAACTCTTGAAGAGCGC created_by: primer3"
     protein_bind    2881..2887
                     /label=SapI Bind
                     /note="created_by: User"
     misc_feature    2889..2891
                     /label=pL1 TX
                     /label=nonstandard type:  gg_overhang
                     /note="created_by: User"
     misc_feature    2898..3467
                     /label=2x cHS4 Insulator
                     /label=nonstandard type:  Insulator
                     /note="created_by: User modified_by: User"
     primer_bind     2898..2920
                     /label=RJ_00516Fa
                     /note="Mismatches: 0 Extension: CAGATGGTCTCTGTTG %GC: 43.5 
                     Hairpin_Tm: 35.8 Self_Dimer_Tm: 10.4 Tm: 60.2 
                     annotation_group: 
                     19159850390528003d770f79-03df-4c9f-96ef-e6fcfd19bc15 
                     Sequence: TTCTGCAATTCATGTGATCTGCG created_by: primer3"
     primer_bind     2898..2920
                     /label=RJ_00516Fb
                     /note="Mismatches: 0 Extension: CAGATGGTCTCTTCGC %GC: 43.5 
                     Hairpin_Tm: 35.8 Self_Dimer_Tm: 10.4 Tm: 60.2 
                     annotation_group: 
                     19159851623847007185a775-ca88-43dd-83ef-f2ae6e814ea6 
                     Sequence: TTCTGCAATTCATGTGATCTGCG created_by: primer3"
     misc_feature    2922..3168
                     /label=cHS4 Core
                     /label=nonstandard type:  Insulator
                     /note="created_by: User"
     protein_bind    2976..2980
                     /label=CTCF Bind (Short)
                     /note="created_by: User"
     misc_feature    3201..3447
                     /label=cHS4 Core
                     /label=nonstandard type:  Insulator
                     /note="created_by: User"
     protein_bind    3255..3259
                     /label=CTCF Bind (Short)
                     /note="created_by: User"
     primer_bind     complement(3446..3467)
                     /label=RJ_00516Rb
                     /note="Mismatches: 0 Extension: CAGATGGTCTCCTG %GC: 50.0 
                     Hairpin_Tm: None Self_Dimer_Tm: None Tm: 60.5 
                     annotation_group: 
                     1915985478341900171e9663-9026-48a0-9944-f43f71ccc931 
                     Sequence: TGCACGTCGAGTTATGATCCAG created_by: primer3"
     primer_bind     complement(3446..3467)
                     /label=RJ_00516Ra
                     /note="Mismatches: 0 Extension: CAGATGGTCTCTAC %GC: 50.0 
                     Hairpin_Tm: None Self_Dimer_Tm: None Tm: 60.5 
                     annotation_group: 
                     19159852903927005175f1ef-e94e-43f8-9666-b62ed7499a91 
                     Sequence: TGCACGTCGAGTTATGATCCAG created_by: primer3"
     misc_feature    3463..3468
                     /label=ApaLI
                     /note="Recognition_pattern: G^TGCAC Methylation: 
                     CpG:blocked, EcoKI:variable annotation_group: GTGCAC note: 
                     Geneious type: restriction site"
     misc_feature    3466..3469
                     /label=Ligation
                     /label=nonstandard type:  ligation
                     /note="created_by: jones"
     primer_bind     3468..3484
                     /label=RJ_00517Fb
                     /note="Mismatches: 0 Extension: CAGATGGTCTCGCA %GC: 64.7 
                     Hairpin_Tm: 67.8 Self_Dimer_Tm: 20.0 Tm: 56.5 
                     annotation_group: 
                     1915985635421800c748aef1-3664-48fc-a16a-4f894379df58 
                     Sequence: CAGGCTCCAGCCTACAC created_by: primer3"
     LTR             3527..3835
                     /label=PB 5' ITR
                     /note="created_by: jones"
     misc_feature    3836..3839
                     /label=TTAA
                     /label=nonstandard type:  motif
                     /note="created_by: jones"
     misc_feature    complement(3871..3889)
                     /label=BP0170
                     /note="created_by: primer3 Sequence: AATAGGCGTATCACGAGGC 
                     Hairpin_Tm: 46.1 Tm: 57.1 Self_Dimer_Tm: 2.4 %GC: 52.6 
                     modified_by: jones note: Geneious type: 
                     primer_bind_reverse"
     protein_bind    3926..3931
                     /label=AatII (post-GG cut)
                     /note="created_by: User"
     protein_bind    complement(3926..3931)
                     /label=AatII (post-GG cut)
                     /note="created_by: User"
     CDS             4063..4872
                     /label=Kan(R)
                     /note="invitrogen: """"1210000"
     CDS             4063..4872
                     /codon_start=1
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      join(5001..5026,1..648)
                     /label=pUC Origin
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcagagc gtgccacctg
      781 gtcgacttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
      841 aaattgacgc atgtgtttta tcgatctgta tatcgaggtt tatttattaa tttgaataga
      901 tattaagttt tattatattt acacttacat actaataata aattcaacaa acaatttatt
      961 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
     1021 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
     1081 tgttgttctg caattcatgt gatctgcgcc tgggagctca cggggacagc ccccccccaa
     1141 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     1201 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     1261 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     1321 gcctgcagac acctgggggg atacggggaa aaagctaggc tgaaagagga tcttagaatg
     1381 acacgcgcct gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac
     1441 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     1501 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     1561 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     1621 tacggggaaa aagctggatc ataactcgac gtgcagtaaa acgacggcca ggcagtacca
     1681 gaagagcccg agtcttgcac tctgtggttt accgagctct tattggtttt caaacttcat
     1741 tgactgtgcc ttaattaata gggataacag ggtaatgtat cacgaggcag aatttcagat
     1801 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagt
     1861 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cactactaga
     1921 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
     1981 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
     2041 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
     2101 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
     2161 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
     2221 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
     2281 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
     2341 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
     2401 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
     2461 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     2521 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     2581 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
     2641 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2701 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2761 ggtgggcctt tctgcgttta tatactagta ggatccatta ccctgttatc cctaaccggt
     2821 atcactagta ttacagaggt aagttataac agtcgcctaa ccacgaagtg ggctggacgc
     2881 gctcttcaag agttcgcttc tgcaattcat gtgatctgcg cctgggagct cacggggaca
     2941 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     3001 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     3061 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     3121 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagctag gctgaaagag
     3181 gatcttagaa tgacacgcgc ctgggagctc acggggacag ccccccccca aagcccccag
     3241 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     3301 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     3361 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     3421 cacctggggg gatacgggga aaaagctgga tcataactcg acgtgcacag gctccagcct
     3481 acacaatcgc tcaagacgtg taatgctttt attatatatt agtcacgata tctataacaa
     3541 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
     3601 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
     3661 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
     3721 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
     3781 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaaa
     3841 agcttggcgt aatcatgaat tcaggaaagg gcctcgtgat acgcctattt ttataggtta
     3901 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgctgg
     3961 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     4021 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     4081 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     4141 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     4201 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     4261 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     4321 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     4381 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     4441 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     4501 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     4561 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     4621 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     4681 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     4741 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     4801 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     4861 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     4921 tgacgggacg gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
