LOCUS       pXTK002_[TetR_tetAR_PartType2]        3811 bp    DNA     circular UNK 29-OCT-2024
DEFINITION  XanthoMoClo Golden Gate Part Plasmid pXTK002 for cloning, contains
            Marker_TetR-tetAR_PartType2.
ACCESSION   pXTK002
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    609..614
                     /standard_name="BsaI Site"
     misc_feature    616..619
                     /standard_name="5' Overhang"
     CDS             complement(620..1270)
                     /standard_name="TetR CDS"
     misc_feature    620..2734
                     /standard_name="TetR_tetAR_PartType2"
     regulatory      1271..1375
                     /note="Geneious type: promoter"
                     /standard_name="Bidirectional tetAR Promoter Region"
     CDS             1376..2575
                     /standard_name="TetA CDS"
     regulatory      2576..2734
                     /note="Geneious type: terminator"
                     /standard_name="TetA Terminator"
     misc_feature    complement(2735..2738)
                     /standard_name="3' Overhang"
     misc_feature    complement(2740..2745)
                     /standard_name="BsaI Site"
     regulatory      2748..2880
                     /note="Geneious type: promoter"
                     /standard_name="bla Promoter"
     CDS             2881..3741
                     /standard_name="bla CDS"
     regulatory      3742..3795
                     /note="Geneious type: terminator"
                     /standard_name="bla Terminator"
     rep_origin      3796..604
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctcaatt caatcgtcac cctttctcgg tccttcaacg ttcctgacaa
      661 cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt
      721 ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct
      781 gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg
      841 ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac
      901 ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc
      961 gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc
     1021 gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat
     1081 gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt
     1141 gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac
     1201 cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca
     1261 actttgtcat gcttgacact ttatcactga taaacataat atgtccacca acttatcagt
     1321 gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa
     1381 acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg
     1441 cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac
     1501 cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct gcgcacctgt
     1561 gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg
     1621 cgccactgtc gactacgcca tcatggcgac agcgcctttc ctttgggttc tctatatcgg
     1681 gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga
     1741 tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt
     1801 cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc
     1861 gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc
     1921 ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc
     1981 gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat
     2041 gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca
     2101 ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc
     2161 ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct
     2221 cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat
     2281 ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc
     2341 aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc
     2401 gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc
     2461 tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct
     2521 gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg
     2581 gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta
     2641 ggagtgcggt tggaacgttg gcccagccag atactcccga tcacgagcag gacgccgatg
     2701 atttgaagcg cactcagcgt ctgatcttaa ttaacccttg agaccagagt agcccgccta
     2761 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
     2821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2881 atgagcattc agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg
     2941 gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg
     3001 cgcgtgggct atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg
     3061 gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc
     3121 cgtgtggatg cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg
     3181 gtggaatata gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg
     3241 tgcagcgcgg cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt
     3301 ggcggtccga aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg
     3361 gatcgttggg aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg
     3421 ccggcagcaa tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca
     3481 agccgccagc aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt
     3541 agcgcgctgc cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc
     3601 cgtggcatta ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat
     3661 accaccggca gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg
     3721 agcctgatta aacattggta aaccgataca attaaaggct ccttttggag cctttttttt
     3781 tggacgaccc ttgtcagatc aaaggatctt c
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