LOCUS       pXTK058_[Con-Pro_sacB-Bacillus_PartType8]        2142 bp    DNA     circular UNK 30-OCT-2024
DEFINITION  XanthoMoClo Golden Gate Part Plasmid pXTK058 for cloning, contains
            Constitutive-Promoter_sacB-Bacillus_PartType8.
ACCESSION   pXTK058
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    609..614
                     /standard_name="BsaI Site"
     misc_feature    616..619
                     /standard_name="5' Overhang"
     misc_feature    620..1065
                     /standard_name="Con-Pro_sacB-Bacillus_PartType8"
     regulatory      620..1065
                     /note="Geneious type: promoter"
                     /standard_name="sacB Promoter"
     misc_feature    complement(1066..1069)
                     /standard_name="3' Overhang"
     misc_feature    complement(1071..1076)
                     /standard_name="BsaI Site"
     regulatory      1079..1211
                     /note="Geneious type: promoter"
                     /standard_name="bla Promoter"
     CDS             1212..2072
                     /standard_name="bla CDS"
     regulatory      2073..2126
                     /note="Geneious type: terminator"
                     /standard_name="bla Terminator"
     rep_origin      2127..604
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctgc acatatacct gccgttcact attatttagt gaaatgagat
      661 attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc aacagaaact
      721 ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta ggcccgtagt
      781 ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc
      841 aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa
      901 gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat
      961 attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga
     1021 agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgaatgt gagaccagag
     1081 tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac
     1141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1201 aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt
     1261 tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc
     1321 aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat
     1381 cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg
     1441 cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc
     1501 agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg
     1561 tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc
     1621 tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg
     1681 tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg
     1741 ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc
     1801 tgaccctggc aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc
     1861 cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg
     1921 aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg
     1981 tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg
     2041 aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga
     2101 gccttttttt ttggacgacc cttgtcagat caaaggatct tc
//
