LOCUS       pXTK125_[Misc_Terminating-Modulator-B_PartType8-11]        2738 bp    DNA     circular SYN 30-OCT-2024
DEFINITION  XanthoMoClo Golden Gate Part Plasmid pXTK125 for cloning, contains
            Misc_Terminating-Modulator-B_PartType8-11.
ACCESSION   TMB_gel_extract
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     misc_feature    609..614
                     /standard_name="BsaI Site"
     misc_feature    616..619
                     /standard_name="PartType7-8_Junction_Overhang"
     misc_feature    620..623
                     /standard_name="PartType4-5_Junction_Overhang"
     misc_feature    complement(628..634)
                     /standard_name="PaqCI Site"
     regulatory      635..800
                     /note="Geneious type: promoter"
                     /standard_name="BBa_J721163 glpT Promoter and RBS"
     CDS             801..1517
                     /standard_name="sfGFP CDS"
     regulatory      1518..1646
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0015 Terminator"
     misc_feature    1647..1653
                     /standard_name="PaqCI Site"
     misc_feature    1658..1661
                     /standard_name="PartType7-8_Junction_Overhang"
     misc_feature    1662..1665
                     /standard_name="PartType11-0_Junction_Overhang"
     misc_feature    complement(1667..1672)
                     /standard_name="BsaI Site"
     regulatory      1675..1807
                     /note="Geneious type: promoter"
                     /standard_name="bla Promoter"
     CDS             1808..2668
                     /standard_name="bla CDS"
     regulatory      2669..2722
                     /note="Geneious type: terminator"
                     /standard_name="bla Terminator"
     rep_origin      2723..604
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga acggtacgca ggtggaaagt gaaacgtgat ttcatgcgtc
      661 attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca tttaatttat
      721 gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct tagctactag
      781 agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
      841 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
      901 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
      961 cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt
     1021 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
     1081 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
     1141 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaggacg
     1201 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg
     1261 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
     1321 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
     1381 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
     1441 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
     1501 atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1561 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1621 ttcgggtggg cctttctgcg tttatacacc tgcgtacgct ggagttgaga ccagagtagc
     1681 ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc
     1741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1801 gaagagtatg agcattcagc attttcgtgt ggcgctgatt ccgttttttg cggcgttttg
     1861 cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg aagatcaact
     1921 gggtgcgcgc gtgggctata ttgaactgga tctgaacagc ggcaaaattc tggaatcttt
     1981 tcgtccggaa gaacgttttc cgatgatgag cacctttaaa gtgctgctgt gcggtgcggt
     2041 tctgagccgt gtggatgcgg gccaggaaca actgggccgt cgtattcatt atagccagaa
     2101 cgatctggtg gaatatagcc cggtgaccga aaaacatctg accgatggca tgaccgtgcg
     2161 tgaactgtgc agcgcggcga ttaccatgag cgataacacc gcggcgaacc tgctgctgac
     2221 gaccattggc ggtccgaaag aactgaccgc gtttctgcat aacatgggcg atcatgtgac
     2281 ccgtctggat cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac
     2341 caccatgccg gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg agctgctgac
     2401 cctggcaagc cgccagcaac tgattgattg gatggaagcg gataaagtgg cgggtccgct
     2461 gctgcgtagc gcgctgccgg ctggctggtt tattgcggat aaaagcggtg cgggcgaacg
     2521 tggcagccgt ggcattattg cggcgctggg cccggatggt aaaccgagcc gtattgtggt
     2581 gatttatacc accggcagcc aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat
     2641 tggcgcgagc ctgattaaac attggtaaac cgatacaatt aaaggctcct tttggagcct
     2701 ttttttttgg acgacccttg tcagatcaaa ggatcttc
//
