LOCUS       Untitled_2        9420 bp DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             232..412
                     /locus_tag="HIV-1 5 LTR"
                     /label=HIV-1 5 LTR
     misc_feature    523..567
                     /locus_tag="HIV-1 psi pack"
                     /label=HIV-1 psi pack
     misc_feature    1077..1310
                     /locus_tag="RRE"
                     /label=RRE
     misc_feature    1884..2134
                     /locus_tag="EFS1 promoter"
                     /label=EFS1 promoter
     misc_feature    1909..2120
                     /locus_tag="EFS_core_promoter"
                     /label=EFS_core_promoter
     misc_feature    complement(1984..1989)
                     /locus_tag="Kozak-site(5)"
                     /label=Kozak-site
                     /label=Kozak-site(5)
     misc_feature    2126..2145
                     /locus_tag="5'Arm"
                     /label=5'Arm
     misc_feature    2140..2145
                     /locus_tag="Kozak-site"
                     /label=Kozak-site
     misc_feature    2143..2145
                     /locus_tag="strong_translatio_initiator"
                     /label=strong_translatio_initiator
     misc_feature    2146..3633
                     /label=CDC25C
     CDS             2149..2175
                     /label=HA tag
     CDS             2179..2205
                     /label=HA tag
     misc_feature    2839..2880
                     /label=substrate_peptide
                     /note="note=""direct strand 
                     translation""translation=""GLYRSPSMPENLNR"""
     misc_feature    2851..2853
                     /label=S214
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2857..2859
                     /label=S216
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2929..2943
                     /label=NLS
                     /note="note=""direct strand 
                     translation""translation=""KVKKK"""
     misc_feature    2998..3000
                     /label=S263
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    3634..3653
                     /locus_tag="3'Arm"
                     /label=3'Arm
     misc_feature    3640..4223
                     /locus_tag="IRES"
                     /label=IRES
                     /label=nonstandard type: Misc
     misc_feature    complement(3891..3902)
                     /locus_tag="GSLQ_linker"
                     /label=GSLQ_linker
     misc_feature    4230..5249
                     /locus_tag="HygroR"
                     /label=HygroR
     misc_feature    5277..5865
                     /locus_tag="WPRE_2"
                     /label=WPRE_2
     misc_feature    5529..5534
                     /locus_tag="Kozak-site(1)"
                     /label=Kozak-site
                     /label=Kozak-site(1)
     misc_feature    5706..5711
                     /locus_tag="Kozak-site(2)"
                     /label=Kozak-site
                     /label=Kozak-site(2)
     CDS             complement(5748..5759)
                     /locus_tag="FactorXa site"
                     /label=FactorXa site
     misc_feature    5937..5989
                     /locus_tag="delta U3"
                     /label=delta U3
     LTR             5990..6170
                     /locus_tag="HIV-1 5 LTR(1)"
                     /label=HIV-1 5 LTR
                     /label=HIV-1 5 LTR(1)
     misc_feature    6409..6486
                     /locus_tag="SV40 ORI"
                     /label=SV40 ORI
     primer_bind     complement(6564..6583)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(6593..6610)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             6681..6749
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      complement(6756..7196)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     misc_feature    complement(7208..7213)
                     /locus_tag="Kozak-site(6)"
                     /label=Kozak-site
                     /label=Kozak-site(6)
     promoter        7267..7295
                     /locus_tag="Amp prom"
                     /label=Amp prom
     CDS             7535..8194
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      8346..8974
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    8538..8543
                     /locus_tag="Kozak-site(3)"
                     /label=Kozak-site
                     /label=Kozak-site(3)
     misc_feature    8895..8900
                     /locus_tag="Kozak-site(4)"
                     /label=Kozak-site
                     /label=Kozak-site(4)
     promoter        9280..9309
                     /locus_tag="lac"
                     /label=lac
     misc_binding    9314..9336
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     9342..9362
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     9380..9399
                     /locus_tag="T3"
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccctacgac
     2161 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggaag cggatctacg
     2221 gaactcttct catccacaag agaggaagga agctctggct caggacccag ttttaggtct
     2281 aatcaaagga aaatgttaaa cctgctcctg gagagagaca cttcctttac cgtctgtcca
     2341 gatgtcccta gaactccagt gggcaaattt cttggtgatt ctgcaaacct aagcattttg
     2401 tctggaggaa ccccaaaacg ttgcctcgat ctttcgaatc ttagcagtgg ggagataact
     2461 gccactcagc ttaccacttc tgcagacctt gatgaaactg gtcacctgga ttcttcagga
     2521 cttcaggaag tgcatttagc tgggatgaat catgaccagc acctaatgaa atgtagccca
     2581 gcacagcttc tttgtagcac tccgaatggt ttggaccgtg gccatagaaa gagagatgca
     2641 atgtgtagtt catctgcaaa taaagaaaat gacaatggaa acttggtgga cagtgaaatg
     2701 aaatatttgg gcagtcccat tactactgtt ccaaaattgg ataaaaatcc aaacctagga
     2761 gaagaccagg cagaagagat ttcagatgaa ttaatggagt tttccctgaa agatcaagaa
     2821 gcaaaggtga gcagaagtgg cctatatcgc tccccgtcga tgccagagaa cttgaacagg
     2881 ccaagactga agcaggtgga aaaattcaag gacaacacaa taccagataa agttaaaaaa
     2941 aagtattttt ctggccaagg aaagctcagg aagggcttat gtttaaagaa gacagtctct
     3001 ctgtgtgaca ttactatcac tcagatgctg gaggaagatt ctaaccaggg gcacctgatt
     3061 ggtgattttt ccaaggtatg tgcgctgcca accgtgtcag ggaaacacca agatctgaag
     3121 tatgtcaacc cagaaacagt ggctgcctta ctgtcgggga agttccaggg tctgattgag
     3181 aagttttatg tcattgattg tcgctatcca tatgagtatc tgggaggaca catccaggga
     3241 gccttaaact tatatagtca ggaagaactg tttaacttct ttctgaagaa gcccatcgtc
     3301 cctttggaca cccagaagag aataatcatc gtgttccact gtgaattctc ctcagagagg
     3361 ggcccccgaa tgtgccgctg tctgcgtgaa gaggacaggt ctctgaacca gtatcctgca
     3421 ttgtactacc cagagctata tatccttaaa ggcggctaca gagacttctt tccagaatat
     3481 atggaactgt gtgaaccaca gagctactgc cctatgcatc atcaggacca caagactgag
     3541 ttgctgaggt gtcgaagcca gagcaaagtg caggaagggg agcggcagct gcgggagcag
     3601 attgcccttc tggtgaagga catgagccca tgagcaattc cgcccctctc cctccccccc
     3661 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3721 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3781 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3841 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3901 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     3961 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     4021 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     4081 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     4141 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     4201 aaaaacacga tgataatatg gccacaacca tgaaaaagcc tgaactcacc gctacctctg
     4261 tcgagaagtt tctgatcgaa aagttcgaca gtgttagcga cctgatgcag ctctccgagg
     4321 gcgaagaatc tcgggctttc agcttcgatg tgggagggcg tggatatgtc ctgcgggtga
     4381 atagctgcgc cgatggtttc tacaaagatc gctatgttta tcggcacttt gcatccgccg
     4441 ctctccctat tcccgaagtg cttgacattg gggagttcag cgagagcctg acctattgca
     4501 tctcccgccg tgcacagggt gtcaccttgc aagacctgcc tgaaaccgaa ctgcccgctg
     4561 ttctccagcc cgtcgccgag gccatggatg ccatcgctgc cgccgatctt agccagacca
     4621 gcgggttcgg cccattcgga cctcaaggaa tcggtcaata cactacatgg cgcgatttca
     4681 tctgcgctat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     4741 gtgcctccgt cgcccaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     4801 tccggcacct cgtgcacgcc gatttcggct ccaacaatgt cctgaccgac aatggccgca
     4861 taacagccgt cattgactgg agcgaggcca tgttcgggga ttcccaatac gaggtcgcca
     4921 acatcttctt ctggaggccc tggttggctt gtatggagca gcagacccgc tacttcgagc
     4981 ggaggcatcc cgagcttgca ggatctcctc ggctccgggc ttatatgctc cgcattggtc
     5041 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggctcagg
     5101 gtcgctgcga cgcaatcgtc cggtccggag ccgggactgt cgggcgtaca caaatcgccc
     5161 gcagaagcgc tgccgtctgg accgatggct gtgtggaagt gctcgccgat agtggaaaca
     5221 gacgccccag cactcgtcct agggcaaagg atctgcagta gacgcgttaa gtcgacaatc
     5281 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5341 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5401 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5461 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5521 ggggcattgc caccacctgt caactccttt ccgggacttt cgctttcccc ctccctattg
     5581 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5641 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5701 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5761 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5821 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     5881 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5941 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6001 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6061 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6121 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     6181 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6241 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6361 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6421 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6541 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6601 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6661 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6721 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6961 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7141 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7201 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7261 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7321 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7381 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7441 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7501 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7561 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7621 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7681 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7741 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7801 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7861 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7921 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7981 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8041 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8101 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8161 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8221 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8281 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8341 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8401 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8461 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8521 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8581 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8641 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8701 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8761 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8821 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8881 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8941 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9001 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9061 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9121 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9181 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9241 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9301 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9361 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
