LOCUS       Untitled_3        8706 bp DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1884..2134
                     /label=EFS1 promoter
     misc_feature    1909..2120
                     /label=EFS_core_promoter
     misc_feature    2143..2145
                     /label=strong_translatio_initiator
     misc_feature    2146..3528
                     /label=TEAD1
     misc_feature    2146..2247
                     /label=3xHA
     CDS             2161..2187
                     /label=HA tag
     CDS             2191..2217
                     /label=HA tag
     CDS             2221..2247
                     /label=HA tag
     misc_feature    2248..2349
                     /label=disordered
                     /note="note=""direct strand 
                     translation""translation=""IEPSSWSGSESPAENMERMSDSADKPIDNDAE
                     GV"""
     misc_feature    2350..2505
                     /label=TEA_domain
                     /note="note=""direct strand 
                     translation""translation=""WSPDIEQSFQEALAIYPPCGRRKIILSDEGKM
                     YGRNELIARYIKLRTGKTRT"""
     misc_feature    2506..2556
                     /label=NLS
                     /note="note=""direct strand 
                     translation""translation=""RKQVSSHIQVLARRKSR"""
     misc_feature    2536..2553
                     /label=MARK_site_L-X-R-X-X-S
                     /note="note=""direct strand 
                     translation""translation=""QVSSHIQVLARRKS"""
     misc_feature    2542..2553
                     /label=CAMPK_site
                     /note="note=""direct strand 
                     translation""translation=""RRKS"""
     misc_feature    2746..3525
                     /label=TAD
                     /note="note=""direct strand 
                     translation""translation=""GSSQDVKPFVQQAYPIQPAVTAPIPGFEPASA
                     PAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESV
                     DIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSE
                     NMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEK
                     "
     misc_feature    complement(3058..3063)
                     /label=Kozak-site
     misc_feature    3535..4118
                     /label=nonstandard type: Misc
     misc_feature    4125..4544
                     /label=BlastR
     misc_feature    4563..5151
                     /label=WPRE
     CDS             complement(5034..5045)
                     /label=FactorXa site
     misc_feature    5223..5275
                     /label=delta U3
     LTR             5276..5456
                     /label=HIV-1 5 LTR
     misc_feature    5695..5772
                     /label=SV40 ORI
     primer_bind     complement(5850..5869)
                     /label=T7
     primer_bind     complement(5879..5896)
                     /label=M13-fwd
     CDS             5967..6035
                     /label=LacZ alpha
     rep_origin      complement(6042..6482)
                     /direction=LEFT
                     /label=F1 ori
     promoter        6553..6581
                     /label=Amp prom
     CDS             6821..7480
                     /label=AmpR
     rep_origin      7632..8260
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        8566..8595
                     /label=lac
     misc_binding    8600..8622
                     /label=LacO
     primer_bind     8628..8648
                     /label=M13-rev
     primer_bind     8666..8685
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatggg tcaacaggga
     2161 tatccctatg acgtaccaga ttacgccgga tatccttacg acgtgcctga ctatgctggg
     2221 tatccgtacg acgtgcccga ttacgctatt gagcccagca gctggagcgg cagtgagagc
     2281 cctgccgaaa acatggaaag gatgagtgac tctgcagata agccaattga caatgatgca
     2341 gaaggggtct ggagccccga catcgagcaa agctttcagg aggccctggc tatctatcca
     2401 ccatgtggga ggaggaaaat catcttatca gacgaaggca aaatgtatgg taggaatgaa
     2461 ttgatagcca gatacatcaa actcaggaca ggcaagacga ggaccagaaa acaggtgtct
     2521 agtcacattc aggttcttgc cagaaggaaa tctcgtgatt ttcattccaa gctaaaggat
     2581 cagactgcaa aggataaggc cctgcagcac atggcggcca tgtcctcagc ccagatcgtc
     2641 tcggccactg ccattcataa caagctgggg ctgcctggga ttccacgccc gaccttccca
     2701 ggggcgccgg ggttctggcc gggaatgatt caaacagggc agccaggatc ctcacaagac
     2761 gtcaagcctt ttgtgcagca ggcctacccc atccagccag cggtcacagc ccccattcca
     2821 gggtttgagc ctgcatcggc cccagctccc tcagtccctg cctggcaagg tcgctccatt
     2881 ggcacaacca agcttcgcct ggtggaattt tcagcttttc tcgagcagca gcgagaccca
     2941 gactcgtaca acaaacacct cttcgtgcac attgggcatg ccaaccattc ttacagtgac
     3001 ccattgcttg aatcagtgga cattcgtcag atttatgaca aatttcctga aaagaaaggt
     3061 ggcttaaagg aactgtttgg aaagggccct caaaatgcct tcttcctcgt aaaattctgg
     3121 gctgatttaa actgcaatat tcaagatgat gctggggctt tttatggtgt aaccagtcag
     3181 tacgagagtt ctgaaaatat gacagtcacc tgttccacca aagtttgctc ctttgggaag
     3241 caagtagtag aaaaagtaga gacggagtat gcaaggtttg agaatggccg atttgtatac
     3301 cgaataaacc gctccccaat gtgtgaatat atgatcaact tcatccacaa gctcaaacac
     3361 ttaccagaga aatatatgat gaacagtgtt ttggaaaact tcacaatttt attggtggta
     3421 acaaacaggg atacacaaga aactctactc tgcatggcct gtgtgtttga agtttcaaat
     3481 agtgaacacg gagcacaaca tcatatttac aggcttgtaa aggactgagc aattccgccc
     3541 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     3601 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3661 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3721 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3781 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3841 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3901 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3961 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     4021 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     4081 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgaag accttcaaca
     4141 tctctcagca ggaccttgag ctggtggaag ttgccactga gaaaatcacc atgctctatg
     4201 aggacaacaa gcaccatgtt ggtgctgcca tcaggaccaa gacaggagaa atcatctctg
     4261 ctgtccacat tgaagcctac attggcaggg tcactgtgtg tgcagaggcc attgccattg
     4321 ggtctgcagt ctccaatggg cagaaggact ttgacaccat tgtggctgtg aggcacccct
     4381 acagtgatga ggtggacagg tccatcagag tggtgtcccc ctgtggcatg tgcagggagc
     4441 tcatctcaga ctatgcccct gattgctttg ttctgattga gatgaatggc aagctggtca
     4501 agacaaccat tgaggagctg attccactga aatacaccag aaactaaacg cgttaagtcg
     4561 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4621 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4681 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4741 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4801 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4861 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4921 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4981 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5041 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5101 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     5161 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5221 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     5461 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5521 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5581 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5641 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5701 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5761 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5821 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     5881 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5941 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6001 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     6061 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6121 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6181 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6241 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6301 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6361 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6421 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6481 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6601 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6661 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6721 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6781 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6841 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6901 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6961 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7021 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7081 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7141 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7201 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7261 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7321 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7381 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7441 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7801 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8281 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8341 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8401 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8461 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8521 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8581 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8641 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     8701 gcaagc
//
