LOCUS       Exported                8904 bp ds-DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 4, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1884..2134
                     /label=EFS1 promoter
     misc_feature    1909..2120
                     /label=EFS_core_promoter
     misc_feature    2143..2145
                     /label=strong_translatio_initiator
     CDS             2146..3726
                     /codon_start=1
                     /label=YAP
                     /translation="MYPYDVPDYAGYPYDVPDYAGSGDPGQQPPPQPAPQGQGQPPSQP
                     PQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDSETDLEALFNAVMNPKTANVPQ
                     TVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGT
                     LTPTGVVSGPAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQ
                     DPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWL
                     DPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERL
                     RLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTM
                     TTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPS
                     QQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKL
                     DKESFLTWL"
     CDS             2149..2175
                     /label=HA tag
     CDS             2179..2205
                     /label=HA tag
     misc_feature    2206..2214
                     /label=linker
                     /note="note=""direct strand
                     translation""translation=""GSG"""
     misc_feature    2215..2388
                     /label=disordered
     misc_feature    2295..2300
                     /label=Kozak-site
     misc_feature    2392..2394
                     /label=5SA
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2395..2511
                     /label=TEAD binding domain
                     /note="note=""direct strand 
                     translation""translation=""ETDLEALFNAVMNPKTANVPQTVPMRLRKLPD
                      SFFKPPE"""
     misc_feature    2482..2553
                     /label=disordered
     misc_feature    2536..2538
                     /label=5SA
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2590..2592
                     /label=5SA
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2593..2595
                     /label=MS_phosphosite_1
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2608..2685
                     /label=disordered
     misc_feature    2698..2700
                     /label=MS_phosphosite_5
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2701..2703
                     /label=5SA
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    2731..2820
                     /label=WW domain 1
     misc_feature    2905..2994
                     /label=WW domain 2
     misc_feature    2974..2976
                     /label=MARK2_site
                     /note="note=""direct strand translation""translation=""T"""
     misc_feature    3034..3138
                     /label=disordered
     misc_feature    3054..3059
                     /label=Kozak-site
     misc_feature    3082..3723
                     /label=transactivation domain
     misc_feature    3103..3288
                     /label=coiled-coil-domain
                     /note="note=""direct strand 
                     translation""translation=""SNSNQQQQMRLQQLQMEKERLRLKQQELLRQA
                      MRNINPSTANSPKCQELALRSQLPTLEQDG"""
     misc_feature    3259..3261
                     /label=MS_phosphosite_2
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    3271..3273
                     /label=MS_phosphosite_3
                     /note="note=""direct strand translation""translation=""T"""
     misc_feature    3400..3402
                     /label=5SA
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    3403..3405
                     /label=MS_phosphosite_4
                     /note="note=""direct strand translation""translation=""T"""
     misc_feature    3747..4316
                     /label=nonstandard type: Misc
     misc_feature    4323..4742
                     /label=BlastR
     misc_feature    4761..5349
                     /label=WPRE
     CDS             complement(5232..5243)
                     /label=FactorXa site
     misc_feature    5421..5473
                     /label=delta U3
     LTR             5474..5654
                     /label=HIV-1 5 LTR
     misc_feature    5893..5970
                     /label=SV40 ORI
     primer_bind     complement(6048..6067)
                     /label=T7
     primer_bind     complement(6077..6094)
                     /label=M13-fwd
     CDS             6165..6233
                     /label=LacZ alpha
     rep_origin      complement(6240..6680)
                     /direction=LEFT
                     /label=F1 ori
     promoter        6751..6779
                     /label=Amp prom
     CDS             7019..7678
                     /label=AmpR
     rep_origin      7830..8458
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        8764..8793
                     /label=lac
     misc_binding    8798..8820
                     /label=LacO
     primer_bind     8826..8846
                     /label=M13-rev
     primer_bind     8864..8883
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtgaccct
     2221 gggcaacagc cccctccgca gccggcacca cagggccagg gacaacctcc gtctcagccg
     2281 cctcaagggc aagggccacc gtcaggacct ggacaacctg cgccagcagc cactcaagcg
     2341 gccccgcagg ctccgcccgc aggacaccaa atagtccacg tgagaggcga ctccgagacg
     2401 gacctcgagg ccctctttaa tgctgttatg aaccctaaga cagcgaacgt gcctcaaact
     2461 gtgcctatgc gattgcgaaa gctgccagat tcctttttca agccgccaga acccaaatcc
     2521 cactcccgcc aggcttccac cgacgctggt acggccgggg cacttactcc ccaacatgtt
     2581 cgggctcata gctcacccgc ttccctccag cttggtgctg tttctccggg gacgctcacc
     2641 ccgaccggtg tagtttccgg acctgctgct actccgactg ctcaacattt gcgccaatcc
     2701 agctttgaaa ttccggacga tgtgcctctg ccggctggct gggaaatggc aaaaacatcc
     2761 agtggtcaaa gatattttct caatcatatt gaccagacga ccacctggca agatccgcgg
     2821 aaagctatgc tctcacagat gaacgttacc gctccgacct ctcctcccgt acaacaaaac
     2881 atgatgaact cagcgtcagg gcccctccca gatggctggg aacaggcaat gactcaggac
     2941 ggcgagattt attacattaa tcacaaaaac aagaccacta gttggctgga ccctcggctt
     3001 gatccacgat tcgctatgaa tcagagaata tcccagagcg ctccggttaa gcagccacct
     3061 cccctggcac cccagagtcc tcaaggcgga gtcatgggcg ggagtaatag caaccagcag
     3121 cagcagatgc gacttcagca actccaaatg gaaaaagaaa gacttaggct gaaacaacag
     3181 gaacttctgc gccaggcaat gcggaacatt aatcccagca cagccaatag tccaaagtgc
     3241 caagaactgg ccttgcgctc acaacttcca acattggagc aagacggagg aactcaaaac
     3301 ccggttagct ctcctggtat gtcccaagag ttgcggacaa tgactacgaa tagtagcgat
     3361 cccttcttga atagtggcac ttaccactca agggacgagt ccaccgacag tgggttgtca
     3421 atgtccagtt atagcgtgcc ccgcacccct gatgacttcc tgaactcagt agacgaaatg
     3481 gacacaggcg acacgatcaa ccaatcaaca cttccttccc agcagaatag attcccagat
     3541 tatctggaag ccatcccggg tacgaacgtc gacttgggta ccttggaagg agatgggatg
     3601 aatatcgaag gtgaggagct gatgccatct ttgcaagaag cattgagctc tgatatcctt
     3661 aacgacatgg agagtgtcct cgccgcgact aagttggaca aagagtcttt cctgacttgg
     3721 ctttgagcaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3781 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3841 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3901 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3961 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4021 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4081 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4141 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4201 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4261 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4321 ccatgaagac cttcaacatc tctcagcagg accttgagct ggtggaagtt gccactgaga
     4381 aaatcaccat gctctatgag gacaacaagc accatgttgg tgctgccatc aggaccaaga
     4441 caggagaaat catctctgct gtccacattg aagcctacat tggcagggtc actgtgtgtg
     4501 cagaggccat tgccattggg tctgcagtct ccaatgggca gaaggacttt gacaccattg
     4561 tggctgtgag gcacccctac agtgatgagg tggacaggtc catcagagtg gtgtccccct
     4621 gtggcatgtg cagggagctc atctcagact atgcccctga ttgctttgtt ctgattgaga
     4681 tgaatggcaa gctggtcaag acaaccattg aggagctgat tccactgaaa tacaccagaa
     4741 actaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4801 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4861 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4921 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4981 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5041 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5101 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5161 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5221 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5281 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5341 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5641 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     5701 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     5761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5821 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     5881 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5941 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6001 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     6061 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6121 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6181 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6241 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6301 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6361 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6421 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6481 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6541 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6601 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6661 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7681 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7741 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7981 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8161 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8221 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8281 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8341 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8401 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8461 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8521 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8581 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8641 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8701 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8761 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8821 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     8881 ggaacaaaag ctggagctgc aagc
//
