LOCUS       Untitled_5        9513 bp DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             232..412
                     /locus_tag="HIV-1 5 LTR"
                     /label=HIV-1 5 LTR
     misc_feature    523..567
                     /locus_tag="HIV-1 psi pack"
                     /label=HIV-1 psi pack
     misc_feature    1077..1310
                     /locus_tag="RRE"
                     /label=RRE
     misc_feature    1884..2125
                     /locus_tag="EFS1 promoter"
                     /label=EFS1 promoter
     misc_feature    1909..2120
                     /locus_tag="EFS_core_promoter"
                     /label=EFS_core_promoter
     misc_feature    complement(1984..1989)
                     /locus_tag="Kozak-site(5)"
                     /label=Kozak-site
                     /label=Kozak-site(5)
     misc_feature    2140..2145
                     /label=Kozak-site
     CDS             2149..2175
                     /label=HA tag
     CDS             2179..2205
                     /label=HA tag
     misc_feature    2206..2214
                     /label=linker
                     /note="note=""direct strand 
                     translation""translation=""GSG"""
     misc_feature    2215..2388
                     /label=disordered
     misc_feature    2295..2300
                     /label=Kozak-site
     misc_feature    2395..2511
                     /label=TEAD binding domain
                     /note="note=""direct strand 
                     translation""translation=""ETDLEALFNAVMNPKTANVPQTVPMRLRKLPD
                     SFFKPPE"""
     misc_feature    2482..2553
                     /label=disordered
     misc_feature    2608..2685
                     /label=disordered
     misc_feature    2731..2820
                     /label=WW domain 1
     misc_feature    2905..2994
                     /label=WW domain 2
     misc_feature    2974..2976
                     /label=MARK2_site
                     /note="note=""direct strand translation""translation=""T"""
     misc_feature    3034..3138
                     /label=disordered
     misc_feature    3054..3059
                     /label=Kozak-site
     misc_feature    3082..3258
                     /label=transactivation domain [*]
     misc_feature    3263..3270
                     /label=transactivation domain [*] [3'] [*]
     misc_feature    3275..3402
                     /label=transactivation domain [*] [3'] [*] [3'] [*]
     misc_feature    3403
                     /label=transactivation domain [*] [3'] [*] [3'] [*] [5']
     misc_feature    3407..3723
                     /label=transactivation domain [*] [3'] [*] [3'] [*] [3']
     misc_feature    3747..4316
                     /locus_tag="IRES"
                     /label=IRES
                     /label=nonstandard type: Misc
     misc_feature    complement(3984..3995)
                     /locus_tag="GSLQ_linker"
                     /label=GSLQ_linker
     misc_feature    4323..5342
                     /locus_tag="HygroR"
                     /label=HygroR
     misc_feature    5370..5958
                     /locus_tag="WPRE_2"
                     /label=WPRE_2
     misc_feature    5622..5627
                     /locus_tag="Kozak-site(1)"
                     /label=Kozak-site
                     /label=Kozak-site(1)
     misc_feature    5799..5804
                     /locus_tag="Kozak-site(2)"
                     /label=Kozak-site
                     /label=Kozak-site(2)
     CDS             complement(5841..5852)
                     /locus_tag="FactorXa site"
                     /label=FactorXa site
     misc_feature    6030..6082
                     /locus_tag="delta U3"
                     /label=delta U3
     LTR             6083..6263
                     /locus_tag="HIV-1 5 LTR(1)"
                     /label=HIV-1 5 LTR
                     /label=HIV-1 5 LTR(1)
     misc_feature    6502..6579
                     /locus_tag="SV40 ORI"
                     /label=SV40 ORI
     primer_bind     complement(6657..6676)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(6686..6703)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             6774..6842
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      complement(6849..7289)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     misc_feature    complement(7301..7306)
                     /locus_tag="Kozak-site(6)"
                     /label=Kozak-site
                     /label=Kozak-site(6)
     promoter        7360..7388
                     /locus_tag="Amp prom"
                     /label=Amp prom
     CDS             7628..8287
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      8439..9067
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    8631..8636
                     /locus_tag="Kozak-site(3)"
                     /label=Kozak-site
                     /label=Kozak-site(3)
     misc_feature    8988..8993
                     /locus_tag="Kozak-site(4)"
                     /label=Kozak-site
                     /label=Kozak-site(4)
     promoter        9373..9402
                     /locus_tag="lac"
                     /label=lac
     misc_binding    9407..9429
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     9435..9455
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     9473..9492
                     /locus_tag="T3"
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtgaccct
     2221 gggcaacagc cccctccgca gccggcacca cagggccagg gacaacctcc gtctcagccg
     2281 cctcaagggc aagggccacc gtcaggacct ggacaacctg cgccagcagc cactcaagcg
     2341 gccccgcagg ctccgcccgc aggacaccaa atagtccacg tgagaggcga ctccgagacg
     2401 gacctcgagg ccctctttaa tgctgttatg aaccctaaga cagcgaacgt gcctcaaact
     2461 gtgcctatgc gattgcgaaa gctgccagat tcctttttca agccgccaga acccaaatcc
     2521 cactcccgcc aggcttccac cgacgctggt acggccgggg cacttactcc ccaacatgtt
     2581 cgggctcata gcgaccccgc ttccctccag cttggtgctg tttctccggg gacgctcacc
     2641 ccgaccggtg tagtttccgg acctgctgct actccgactg ctcaacattt gcgccaagac
     2701 agctttgaga ttccggacga tgtgcctctg ccggctggct gggaaatggc aaaaacatcc
     2761 agtggtcaaa gatattttct caatcatatt gaccagacga ccacctggca agatccgcgg
     2821 aaagctatgc tctcacagat gaacgttacc gctccgacct ctcctcccgt acaacaaaac
     2881 atgatgaact cagcgtcagg gcccctccca gatggctggg aacaggcaat gactcaggac
     2941 ggcgagattt attacattaa tcacaaaaac aagaccacta gttggctgga ccctcggctt
     3001 gatccacgat tcgctatgaa tcagagaata tcccagagcg ctccggttaa gcagccacct
     3061 cccctggcac cccagagtcc tcaaggcgga gtcatgggcg ggagtaatag caaccagcag
     3121 cagcagatgc gacttcagca actccaaatg gaaaaagaaa gacttaggct gaaacaacag
     3181 gaacttctgc gccaggcaat gcggaacatt aatcccagca cagccaatag tccaaagtgc
     3241 caagaactgg ccttgcgcga ccaacttcca gacttggagc aagacggagg aactcaaaac
     3301 ccggttagct ctcctggtat gtcccaagag ttgcggacaa tgactacgaa tagtagcgat
     3361 cccttcttga atagtggcac ttaccactca agggacgagt ccgacgacag tgggttgtca
     3421 atgtccagtt atagcgtgcc ccgcacccct gatgacttcc tgaactcagt agacgaaatg
     3481 gacacaggcg acacgatcaa ccaatcaaca cttccttccc agcagaatag attcccagat
     3541 tatctggaag ccatcccggg tacgaacgtc gacttgggta ccttggaagg agatgggatg
     3601 aatatcgaag gtgaggagct gatgccatct ttgcaagaag cattgagctc tgatatcctt
     3661 aacgacatgg agagtgtcct cgccgcgact aagttggaca aagagtcttt cctgacttgg
     3721 ctttgagcaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3781 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3841 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3901 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3961 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4021 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4081 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4141 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4201 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4261 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4321 ccatgaaaaa gcctgaactc accgctacct ctgtcgagaa gtttctgatc gaaaagttcg
     4381 acagtgttag cgacctgatg cagctctccg agggcgaaga atctcgggct ttcagcttcg
     4441 atgtgggagg gcgtggatat gtcctgcggg tgaatagctg cgccgatggt ttctacaaag
     4501 atcgctatgt ttatcggcac tttgcatccg ccgctctccc tattcccgaa gtgcttgaca
     4561 ttggggagtt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacct
     4621 tgcaagacct gcctgaaacc gaactgcccg ctgttctcca gcccgtcgcc gaggccatgg
     4681 atgccatcgc tgccgccgat cttagccaga ccagcgggtt cggcccattc ggacctcaag
     4741 gaatcggtca atacactaca tggcgcgatt tcatctgcgc tattgctgat ccccatgtgt
     4801 atcactggca aactgtgatg gacgacaccg tcagtgcctc cgtcgcccag gctctcgatg
     4861 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gccgatttcg
     4921 gctccaacaa tgtcctgacc gacaatggcc gcataacagc cgtcattgac tggagcgagg
     4981 ccatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccctggttgg
     5041 cttgtatgga gcagcagacc cgctacttcg agcggaggca tcccgagctt gcaggatctc
     5101 ctcggctccg ggcttatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     5161 acggcaattt cgatgatgca gcttgggctc agggtcgctg cgacgcaatc gtccggtccg
     5221 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgctgccgtc tggaccgatg
     5281 gctgtgtgga agtgctcgcc gatagtggaa acagacgccc cagcactcgt cctagggcaa
     5341 aggatctgca gtagacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     5401 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5461 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5521 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5581 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcaactcc
     5641 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5701 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5761 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5821 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5881 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5941 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     6001 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6061 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6121 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6181 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6241 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     6301 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6361 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6421 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6481 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6541 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6601 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6661 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6721 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6781 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6841 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6901 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6961 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7021 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7081 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7141 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7201 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7261 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7321 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7381 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7441 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7501 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7561 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7621 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7681 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7741 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7801 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7861 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7921 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7981 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8041 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8101 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8161 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8221 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8281 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9301 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9361 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9421 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     9481 tcactaaagg gaacaaaagc tggagctgca agc
//
