LOCUS       FLAG_MARK2_K82H_        9537 bp DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1884..2125
                     /label=EFS1 promoter
     misc_feature    1909..2120
                     /label=EFS_core_promoter
     misc_feature    2126..4379
                     /label=MARK2
     misc_feature    2140..2145
                     /label=Kozak-site
     misc_feature    2146..2172
                     /label=FLAG_tag
     CDS             2149..2172
                     /label=Flag tag
     misc_feature    2353..2562
                     /label=Kinase_domain [*]
                     /note="note=""direct strand 
                     translation""translation=""IGKGNFAKVKLARHILTGREVAIKIIDKTQLN
                     PTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKE
                     KEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDT
                     FCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY
                     "
     misc_feature    2422..2424
                     /label=ATP-LYSINE
                     /note="note=""direct strand translation""translation=""K"""
     misc_feature    2563..2565
                     /label=GK_mut
     misc_feature    2563
                     /label=Kinase_domain [*] [*] [5']
                     /note="note=""direct strand 
                     translation""translation=""IGKGNFAKVKLARHILTGREVAIKIIDKTQLN
                     PTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKE
                     KEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDT
                     FCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY
                     "
     misc_feature    2567..2675
                     /label=Kinase_domain [*] [*] [3']
                     /note="note=""direct strand 
                     translation""translation=""IGKGNFAKVKLARHILTGREVAIKIIDKTQLN
                     PTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKE
                     KEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDT
                     FCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY
                     "
     misc_feature    2676
                     /label=Kinase_domain [*] [5']
                     /note="note=""direct strand 
                     translation""translation=""IGKGNFAKVKLARHILTGREVAIKIIDKTQLN
                     PTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKE
                     KEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDT
                     FCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY
                     "
     misc_feature    2679..3078
                     /label=Kinase_domain [*] [3']
                     /note="note=""direct strand 
                     translation""translation=""IGKGNFAKVKLARHILTGREVAIKIIDKTQLN
                     PTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKE
                     KEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDT
                     FCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY
                     "
     misc_feature    2679..2698
                     /label=sg_5
                     /note="note=""direct strand 
                     translation""translation=""SETDRTX"""
     misc_feature    2788..2807
                     /label=sg_2
                     /note="note=""direct strand 
                     translation""translation=""GKLDTFX"""
     misc_feature    2818..2837
                     /label=sg_6
                     /note="note=""direct strand 
                     translation""translation=""PYAAPEX"""
     misc_feature    3046..3065
                     /label=sg_4
                     /note="note=""direct strand 
                     translation""translation=""IKRGTLX"""
     misc_feature    4262..4281
                     /label=sg_1
                     /note="note=""direct strand 
                     translation""translation=""DCL*"""
     misc_feature    4284..4303
                     /label=sg_3
                     /note="note=""direct strand 
                     translation""translation=""V*"""
     misc_feature    4380..4949
                     /label=nonstandard type: Misc
     misc_feature    4956..5375
                     /label=BlastR
     misc_feature    5394..5982
                     /label=WPRE
     CDS             complement(5865..5876)
                     /label=FactorXa site
     misc_feature    6054..6106
                     /label=delta U3
     LTR             6107..6287
                     /label=HIV-1 5 LTR
     misc_feature    6526..6603
                     /label=SV40 ORI
     primer_bind     complement(6681..6700)
                     /label=T7
     primer_bind     complement(6710..6727)
                     /label=M13-fwd
     CDS             6798..6866
                     /label=LacZ alpha
     rep_origin      complement(6873..7313)
                     /direction=LEFT
                     /label=F1 ori
     promoter        7384..7412
                     /label=Amp prom
     CDS             7652..8311
                     /label=AmpR
     rep_origin      8463..9091
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        9397..9426
                     /label=lac
     misc_binding    9431..9453
                     /label=LacO
     primer_bind     9459..9479
                     /label=M13-rev
     primer_bind     9497..9516
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac
     2161 gatgacgata agtccactag gaccccattg ccaacggtga atgaacgaga cactgaaaac
     2221 cacacgtcac atggagatgg gcgtcaagaa gttacctctc gtaccagccg ctcaggagct
     2281 cggtgtagaa actctatagc ctcctgtgca gatgaacaac ctcacatcgg aaactacaga
     2341 ctgttgaaaa caatcggcaa ggggaatttt gcaaaagtaa aattggcaag acatatcctt
     2401 acaggcagag aggttgcaat aaaaataatt gacaaaactc agttgaatcc aacaagtcta
     2461 caaaagctct tcagagaagt aagaataatg aagattttaa atcatcccaa tatagtgaag
     2521 ttattcgaag tcattgaaac tgaaaaaaca ctctacctaa tcggtgaata tgcaagtgga
     2581 ggtgaagtat ttgactattt ggttgcacat ggcaggatga aggaaaaaga agcaagatct
     2641 aaatttagac agattgtgtc tgcagttcaa tactgtcatc agaaacggat cgtacatcga
     2701 gacctcaagg ctgaaaatct attgttagat gccgatatga acattaaaat agcagatttc
     2761 ggttttagca atgaatttac tgttggcggt aaactcgaca cgttttgtgg cagtcctcca
     2821 tacgcagcac ctgagctctt ccagggcaag aaatatgacg ggccagaagt ggatgtgtgg
     2881 agtctggggg tcattttata cacactagtc agtggctcac ttccctttga tgggcaaaac
     2941 ctaaaggaac tgagagagag agtattaaga gggaaataca gaattccctt ctacatgtct
     3001 acagactgtg aaaaccttct caaacgtttc ctggtgctaa atccaattaa acgcggcact
     3061 ctagagcaaa tcatgaagga caggtggatc aatgcagggc atgaagaaga tgaactcaaa
     3121 ccatttgttg aaccagagct agacatctca gaccaaaaaa gaatagatat tatggtggga
     3181 atgggatatt cacaagaaga aattcaagaa tctcttagta agatgaaata cgatgaaatc
     3241 acagctacat atttgttatt ggggagaaaa tcttcagagc tggatgctag tgattccagt
     3301 tctagcagca atctttcact tgctaaggtt aggccgagca gtgatctcaa caacagtact
     3361 ggccagtctc ctcaccacaa agtgcagaga agtgtttctt caagccaaaa gcaaagacgc
     3421 tacagtgacc atgctggacc agctattcct tctgttgtgg cgtatccgaa aaggagtcag
     3481 accagcactg cagatagtga cctcaaagaa gatggaattt cctcccggaa atcaagtggc
     3541 agtgctgttg gaggaaaggg aattgctcca gccagtccca tgcttgggaa tgcaagtaat
     3601 cctaataagg cggatattcc tgaacgcaag aaaagctcca ctgtccctag tagtaacaca
     3661 gcatctggtg gaatgacacg acgaaatact tatgtttgca gtgagagaac tacagctgat
     3721 agacactcag tgattcagaa tggcaaagaa aacagcacta ttcctgatca gagaactcca
     3781 gttgcttcaa cacacagtat cagtagtgca gccaccccag atcgaatccg cttcccaaga
     3841 ggcactgcca gtcgtagcac tttccacggc cagccccggg aacggcgaac cgcaacatat
     3901 aatggccctc ctgcctctcc cagcctgtcc catgaagcca caccattgtc ccagactcga
     3961 agccgaggct ccactaatct ctttagtaaa ttaacttcaa aactcacaag gagtcgcaat
     4021 gtatctgctg agcaaaaaga tgaaaacaaa gaagcaaagc ctcgatccct acgcttcacc
     4081 tggagcatga aaaccactag ttcaatggat cccggggaca tgatgcggga aatccgcaaa
     4141 gtgttggacg ccaataactg cgactatgag cagagggagc gcttcttgct cttctgcgtc
     4201 cacggagatg ggcacgcgga gaacctcgtg cagtgggaaa tggaagtgtg caagctgcca
     4261 agactgtctc tgaacggggt ccggtttaag cggatatcgg ggacatccat agccttcaaa
     4321 aatattgctt ccaaaattgc caatgagcta aagctgtgag caattccgcc cctctccctc
     4381 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4441 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4501 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4561 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4621 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4681 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4741 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4801 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4861 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     4921 cctttgaaaa acacgatgat aatatggcca caaccatgaa gaccttcaac atctctcagc
     4981 aggaccttga gctggtggaa gttgccactg agaaaatcac catgctctat gaggacaaca
     5041 agcaccatgt tggtgctgcc atcaggacca agacaggaga aatcatctct gctgtccaca
     5101 ttgaagccta cattggcagg gtcactgtgt gtgcagaggc cattgccatt gggtctgcag
     5161 tctccaatgg gcagaaggac tttgacacca ttgtggctgt gaggcacccc tacagtgatg
     5221 aggtggacag gtccatcaga gtggtgtccc cctgtggcat gtgcagggag ctcatctcag
     5281 actatgcccc tgattgcttt gttctgattg agatgaatgg caagctggtc aagacaacca
     5341 ttgaggagct gattccactg aaatacacca gaaactaaac gcgttaagtc gacaatcaac
     5401 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5461 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5521 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5581 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5641 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5701 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5761 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     5821 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5881 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5941 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     6001 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6061 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6121 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6181 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6241 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     6301 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6361 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6481 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     6541 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6601 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6661 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     6721 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6781 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6841 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     6901 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6961 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7021 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7081 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7141 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7201 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7261 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7321 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7381 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7441 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7501 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7561 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7621 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7681 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7741 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7801 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7861 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7921 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7981 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8041 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8101 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8161 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8221 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8281 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8341 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8401 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8461 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9001 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9061 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9241 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9301 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9361 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9421 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     9481 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagc
//
