LOCUS       Exported                7921 bp ds-DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 4, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..413
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d...
                     /note="label=""5'LTR"" - note=""ApEinfo_revcolor=#bc8dbf""
                     - note=""ApEinfo_fwdcolor=#bc8dbf"" - 1..413"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    464..601
                     /label=psi
                     /note="label=""psi"" - 464..601"
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    568..932
                     /label=gag
                     /note="label=""gag"" - 568..932"
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1078..1319
                     /label=RRE
                     /note="label=""RRE"" - 1078..1319"
     misc_feature    1694..1713
                     /label=pre-hU6_SEQ
     promoter        1717..1941
                     /label=hU6
                     /note="label=""hU6"" - 1717..1965"
     misc_feature    1717..1941
                     /label=hU6 promoter
     misc_feature    1717..1739
                     /label=SANGER SEQ primer
     misc_feature    1884..1924
                     /label=pool screening F_misc_feature
                     /note="note=""pool screening F"" - 1884..1924"
     misc_feature    1917..1941
                     /label=cloning_fwd_primer
     misc_feature    2422..2504
                     /label=sgRNA backbone_misc_feature
                     /note="note=""sgRNA backbone"" - 3847..3949"
     misc_feature    2422..2504
                     /label=sg_scaffold_LRG
     misc_feature    2422..2451
                     /label=cloning_rev_primer
     misc_feature    2546..2663
                     /label=cPPT
                     /note="label=""cPPT"" - 3991..4108"
     misc_feature    2575..2599
                     /label=pool screening R (2nd round)_misc_feature
                     /note="note=""pool screening R (2nd round)"" - 4020..4044"
     misc_feature    2585..2628
                     /label=pool screening R_misc_feature
                     /note="note=""pool screening R"" - 4030..4073"
     promoter        2707..2957
                     /label=EFS
                     /note="label=""EFS"" - 4152..4402"
     misc_feature    2707..2957
                     /label=EFS1 promoter
     misc_feature    2732..2943
                     /label=EFS_core_promoter
     misc_feature    complement(2807..2812)
                     /label=Kozak-site
     misc_feature    2963..2968
                     /label=Kozak-site
     misc_feature    2969..3760
                     /label=NEO
     CDS             2972..3763
                     /label=Kan/neoR
     misc_feature    2996..3016
                     /label=GFP_misc_feature [5']
                     /note="note=""GFP"" - 4441..5160"
     misc_feature    3065..3763
                     /label=GFP_misc_feature [3']
                     /note="note=""GFP"" - 4441..5160"
     misc_binding    3778..4366
                     /label=WPRE
                     /note="bound_moiety="""" - label=""WPRE"" - 5175..5763"
     misc_feature    3778..4366
                     /label=WPRE
     misc_feature    4030..4035
                     /label=Kozak-site
     misc_feature    4207..4212
                     /label=Kozak-site
     CDS             complement(4249..4260)
                     /label=FactorXa site
     LTR             4437..4672
                     /label=3' LTR SIN
                     /note="label=""3' LTR SIN"" - 5834..6069"
     misc_feature    4438..4490
                     /label=delta U3
     LTR             4491..4671
                     /label=HIV-1 5 LTR
     polyA_signal    4749..4879
                     /label=SV40 polyadenylation signal
                     /note="label=""SV40 polyadenylation signal"" - 6146..6276"
     misc_feature    4910..4987
                     /label=SV40 ORI
     rep_origin      4959..5034
                     /direction=RIGHT
                     /label=SV40 origin of replication
                     /note="label=""SV40 origin of replication"" - 6356..6431"
     primer_bind     complement(5065..5084)
                     /label=T7
     primer_bind     complement(5094..5111)
                     /label=M13-fwd
     CDS             5182..5250
                     /label=LacZ alpha
     rep_origin      5252..5707
                     /direction=RIGHT
                     /label=F1 ori
                     /note="label=""F1 ori"" - 6649..7104"
     rep_origin      complement(5257..5697)
                     /direction=LEFT
                     /label=F1 ori
     misc_feature    complement(5709..5714)
                     /label=Kozak-site
     promoter        5768..5796
                     /label=Amp prom
     CDS             5838..6698
                     /label=AmpR
                     /note="label=""AmpR"" - 7235..8095"
     CDS             6036..6695
                     /label=AmpR
     rep_origin      6846..7513
                     /direction=RIGHT
                     /label=pUC ori
                     /note="label=""pUC ori"" - 8243..8910"
     rep_origin      6847..7475
                     /direction=RIGHT
                     /label=ColE1 origin
     misc_feature    7039..7044
                     /label=Kozak-site
     misc_feature    7396..7401
                     /label=Kozak-site
     promoter        7781..7810
                     /label=lac
     misc_binding    7815..7837
                     /label=LacO
     primer_bind     7843..7863
                     /label=M13-rev
     primer_bind     7881..7900
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgactg ccatagaaat
     1981 ggctgagttt cagagctatg cacaagagtg catagcaagt tgaaataagg ctagtccgtt
     2041 atcaactttg ctggaaacag caaagtggca ccgagtcggt gctttttttg cgtgaccgag
     2101 cttgtctgcc cgcgcagtcc actagacaga cgcgcagagg tcgggcgggc ctagggccaa
     2161 tttcccatgg ttcccttcat ttgcatatat gatgtaataa ggttatggag actattaaac
     2221 ttagccctaa tcaaactata tgatgataat gtgtgtggta caaaaggtca taacttatta
     2281 tatactttga aacttaaaaa agggttacag tttagtcacc ataactgtaa aattttttct
     2341 attcttagct ttatatagtt cttgagaggc catgtttatc gacctcagga gccatccaaa
     2401 agtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     2461 ctttgctgga aacagcaaag tggcaccgag tcggtgcttt ttttgaattc tagatcttga
     2521 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2581 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2641 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     2701 gccggctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 caggtgtcgt gacgcgggat ccgccaccat gattgaacaa gatggattgc acgcaggttc
     3001 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3061 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3121 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3181 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3241 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3301 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3361 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3421 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3481 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3541 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3601 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3661 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3721 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgacgcgtta agtcgacaat
     3781 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3841 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3901 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3961 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4021 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4081 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4141 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4201 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4261 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4321 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4381 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4441 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4501 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4561 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4621 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4681 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4741 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4801 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4861 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4921 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4981 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5041 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     5101 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5161 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5221 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5701 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5761 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5821 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5881 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5941 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6001 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6061 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6121 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6181 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6241 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6301 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6361 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6421 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6481 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6541 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6601 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6661 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6721 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6781 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6841 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6901 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6961 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7021 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7081 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7141 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7201 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7261 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7321 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7381 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7441 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7501 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7561 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7621 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7681 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7741 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7801 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7861 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7921 c
//
