LOCUS       Exported                7549 bp ds-DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 4, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..413
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d...
                     /note="label=""5'LTR"" - note=""ApEinfo_revcolor=#bc8dbf""
                     - note=""ApEinfo_fwdcolor=#bc8dbf"" - 1..413"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    464..601
                     /label=psi
                     /note="label=""psi"" - 464..601"
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    568..932
                     /label=gag
                     /note="label=""gag"" - 568..932"
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1078..1319
                     /label=RRE
                     /note="label=""RRE"" - 1078..1319"
     misc_feature    1694..1713
                     /label=pre-hU6_SEQ
     promoter        1717..1941
                     /label=hU6
                     /note="label=""hU6"" - 1717..1965"
     misc_feature    1717..1941
                     /label=hU6 promoter
     misc_feature    1717..1739
                     /label=SANGER SEQ primer
     misc_feature    1884..1924
                     /label=pool screening F_misc_feature
                     /note="note=""pool screening F"" - 1884..1924"
     misc_feature    1917..1941
                     /label=cloning_fwd_primer
     misc_feature    2422..2504
                     /label=sgRNA backbone_misc_feature
                     /note="note=""sgRNA backbone"" - 3847..3949"
     misc_feature    2422..2504
                     /label=sg_scaffold_LRG
     misc_feature    2422..2451
                     /label=cloning_rev_primer
     misc_feature    2546..2663
                     /label=cPPT
                     /note="label=""cPPT"" - 3991..4108"
     misc_feature    2575..2599
                     /label=pool screening R (2nd round)_misc_feature
                     /note="note=""pool screening R (2nd round)"" - 4020..4044"
     misc_feature    2585..2628
                     /label=pool screening R_misc_feature
                     /note="note=""pool screening R"" - 4030..4073"
     promoter        2707..2957
                     /label=EFS
                     /note="label=""EFS"" - 4152..4402"
     misc_feature    2707..2957
                     /label=EFS1 promoter
     misc_feature    2732..2943
                     /label=EFS_core_promoter
     misc_feature    complement(2807..2812)
                     /label=Kozak-site
     misc_feature    2963..2968
                     /label=Kozak-site
     misc_feature    2968..3391
                     /label=GFP_misc_feature [*]
                     /note="note=""GFP"" - 4441..5160"
     misc_feature    2969..3388
                     /label=BlastR
     misc_binding    3406..3994
                     /label=WPRE
                     /note="bound_moiety="""" - label=""WPRE"" - 5175..5763"
     misc_feature    3406..3994
                     /label=WPRE
     misc_feature    3658..3663
                     /label=Kozak-site
     misc_feature    3835..3840
                     /label=Kozak-site
     CDS             complement(3877..3888)
                     /label=FactorXa site
     LTR             4065..4300
                     /label=3' LTR SIN
                     /note="label=""3' LTR SIN"" - 5834..6069"
     misc_feature    4066..4118
                     /label=delta U3
     LTR             4119..4299
                     /label=HIV-1 5 LTR
     polyA_signal    4377..4507
                     /label=SV40 polyadenylation signal
                     /note="label=""SV40 polyadenylation signal"" - 6146..6276"
     misc_feature    4538..4615
                     /label=SV40 ORI
     rep_origin      4587..4662
                     /direction=RIGHT
                     /label=SV40 origin of replication
                     /note="label=""SV40 origin of replication"" - 6356..6431"
     primer_bind     complement(4693..4712)
                     /label=T7
     primer_bind     complement(4722..4739)
                     /label=M13-fwd
     CDS             4810..4878
                     /label=LacZ alpha
     rep_origin      4880..5335
                     /direction=RIGHT
                     /label=F1 ori
                     /note="label=""F1 ori"" - 6649..7104"
     rep_origin      complement(4885..5325)
                     /direction=LEFT
                     /label=F1 ori
     misc_feature    complement(5337..5342)
                     /label=Kozak-site
     promoter        5396..5424
                     /label=Amp prom
     CDS             5466..6326
                     /label=AmpR
                     /note="label=""AmpR"" - 7235..8095"
     CDS             5664..6323
                     /label=AmpR
     rep_origin      6474..7141
                     /direction=RIGHT
                     /label=pUC ori
                     /note="label=""pUC ori"" - 8243..8910"
     rep_origin      6475..7103
                     /direction=RIGHT
                     /label=ColE1 origin
     misc_feature    6667..6672
                     /label=Kozak-site
     misc_feature    7024..7029
                     /label=Kozak-site
     promoter        7409..7438
                     /label=lac
     misc_binding    7443..7465
                     /label=LacO
     primer_bind     7471..7491
                     /label=M13-rev
     primer_bind     7509..7528
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgctaa gcgatcttcg
     1981 ggtcctgttt cagagctatg cacaagagtg catagcaagt tgaaataagg ctagtccgtt
     2041 atcaactttg ctggaaacag caaagtggca ccgagtcggt gctttttttg cgtgaccgag
     2101 cttgtctgcc cgcgcagtcc actagacaga cgcgcagagg tcgggcgggc ctagggccaa
     2161 tttcccatgg ttcccttcat ttgcatatat gatgtaataa ggttatggag actattaaac
     2221 ttagccctaa tcaaactata tgatgataat gtgtgtggta caaaaggtca taacttatta
     2281 tatactttga aacttaaaaa agggttacag tttagtcacc ataactgtaa aattttttct
     2341 attcttagct ttatatagtt cttgagaggc catgtttatc gcattgtcat caaagaacct
     2401 tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     2461 ctttgctgga aacagcaaag tggcaccgag tcggtgcttt ttttgaattc tagatcttga
     2521 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2581 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2641 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     2701 gccggctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 caggtgtcgt gacgcgggat ccgccaccat gaagaccttc aacatctctc agcaggacct
     3001 tgagctggtg gaagttgcca ctgagaaaat caccatgctc tatgaggaca acaagcacca
     3061 tgttggtgct gccatcagga ccaagacagg agaaatcatc tctgctgtcc acattgaagc
     3121 ctacattggc agggtcactg tgtgtgcaga ggccattgcc attgggtctg cagtctccaa
     3181 tgggcagaag gactttgaca ccattgtggc tgtgaggcac ccctacagtg atgaggtgga
     3241 caggtccatc agagtggtgt ccccctgtgg catgtgcagg gagctcatct cagactatgc
     3301 ccctgattgc tttgttctga ttgagatgaa tggcaagctg gtcaagacaa ccattgagga
     3361 gctgattcca ctgaaataca ccagaaacta acgcgttaag tcgacaatca acctctggat
     3421 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3481 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3541 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3601 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3661 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3721 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3781 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3841 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3901 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3961 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     4021 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4081 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4141 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4201 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4261 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     4321 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4501 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4561 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4621 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4681 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4741 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4801 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4861 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5101 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5161 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5221 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5341 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5401 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5461 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5521 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5581 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5641 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5701 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5761 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5821 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5881 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5941 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6001 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6061 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6121 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6181 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6241 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6301 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6361 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6421 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6481 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6541 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6601 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6661 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6721 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6781 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6841 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6901 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6961 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7021 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7081 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7141 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7201 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7261 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7321 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7381 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7441 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7501 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
