LOCUS       sgMARK3-R-1        8364 bp DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..413
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d...
                     /note="label=""5'LTR"" - note=""ApEinfo_revcolor=#bc8dbf""
                     - note=""ApEinfo_fwdcolor=#bc8dbf"" - 1..413"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    464..601
                     /label=psi
                     /note="label=""psi"" - 464..601"
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    568..932
                     /label=gag
                     /note="label=""gag"" - 568..932"
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1078..1319
                     /label=RRE
                     /note="label=""RRE"" - 1078..1319"
     misc_feature    1694..1713
                     /label=pre-hU6_SEQ
     promoter        1717..1941
                     /label=hU6
                     /note="label=""hU6"" - 1717..1965"
     misc_feature    1717..1739
                     /label=SANGER SEQ primer
     misc_feature    1884..1924
                     /label=pool screening F_misc_feature
                     /note="note=""pool screening F"" - 1884..1924"
     misc_feature    1917..1941
                     /label=cloning_fwd_primer
     misc_feature    2421..2503
                     /label=sgRNA backbone_misc_feature
                     /note="note=""sgRNA backbone"" - 3847..3949"
     misc_feature    2421..2450
                     /label=cloning_rev_primer
     misc_feature    2545..2662
                     /label=cPPT
                     /note="label=""cPPT"" - 3991..4108"
     misc_feature    2574..2598
                     /label=pool screening R (2nd round)_misc_feature
                     /note="note=""pool screening R (2nd round)"" - 4020..4044"
     misc_feature    2584..2627
                     /label=pool screening R_misc_feature
                     /note="note=""pool screening R"" - 4030..4073"
     promoter        2706..2956
                     /label=EFS
                     /note="label=""EFS"" - 4152..4402"
     CDS             2971..2994
                     /label=Flag tag
     CDS             2998..3711
                     /label=EGFP
     misc_feature    3718..3783
                     /label=P2A
     misc_feature    3784..4206
                     /label=BlastR
     misc_binding    4221..4809
                     /label=WPRE
                     /note="bound_moiety="""" - label=""WPRE"" - 5175..5763"
     CDS             complement(4692..4703)
                     /label=FactorXa site
     LTR             4880..5115
                     /label=3' LTR SIN
                     /note="label=""3' LTR SIN"" - 5834..6069"
     misc_feature    4881..4933
                     /label=delta U3
     LTR             4934..5114
                     /label=HIV-1 5 LTR
     polyA_signal    5192..5322
                     /label=SV40 polyadenylation signal
                     /note="label=""SV40 polyadenylation signal"" - 6146..6276"
     misc_feature    5353..5430
                     /label=SV40 ORI
     rep_origin      5402..5477
                     /direction=RIGHT
                     /label=SV40 origin of replication
                     /note="label=""SV40 origin of replication"" - 6356..6431"
     primer_bind     complement(5508..5527)
                     /label=T7
     primer_bind     complement(5537..5554)
                     /label=M13-fwd
     CDS             5625..5693
                     /label=LacZ alpha
     rep_origin      5695..6150
                     /direction=RIGHT
                     /label=F1 ori
                     /note="label=""F1 ori"" - 6649..7104"
     rep_origin      complement(5700..6140)
                     /direction=LEFT
                     /label=F1 ori
     promoter        6211..6239
                     /label=Amp prom
     CDS             6281..7141
                     /label=AmpR
                     /note="label=""AmpR"" - 7235..8095"
     CDS             6479..7138
                     /label=AmpR
     rep_origin      7289..7956
                     /direction=RIGHT
                     /label=pUC ori
                     /note="label=""pUC ori"" - 8243..8910"
     rep_origin      7290..7918
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        8224..8253
                     /label=lac
     misc_binding    8258..8280
                     /label=LacO
     primer_bind     8286..8306
                     /label=M13-rev
     primer_bind     8324..8343
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgccgg cgccgagccg
     1981 gacttcgttt cagagctatg cacaagagtg catagcaagt tgaaataagg ctagtccgtt
     2041 atcaactttg ctggaaacag caaagtggca ccgagtcggt gctttttttg cgtgaccgag
     2101 cttgtctgcc cgcgcagtcc actagacaga cgcgcagagg tcgggcgggc ctagggccaa
     2161 tttcccatgg ttcccttcat ttgcatatat gatgtaataa ggttatggag actattaaac
     2221 ttagccctaa tcaaactata tgatgataat gtgtgtggta caaaaggtca taacttatta
     2281 tatactttga aacttaaaaa agggttacag tttagtcacc ataactgtaa aattttttct
     2341 attcttagct ttatatagtt cttgagaggc catgtttatc gactgtctct gaacggggtc
     2401 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
     2461 tttgctggaa acagcaaagt ggcaccgagt cggtgctttt tttgaattct agatcttgag
     2521 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2581 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2641 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2701 ccggctcgag tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2761 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2821 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2881 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2941 aggtgtcgtg acgcgggatc cgccaccatg gattacaaag acgatgacga taagatggtg
     3001 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3061 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3121 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3181 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3241 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3301 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3361 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3421 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3481 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3541 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3601 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3661 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggctagcggc
     3721 agcggcgcca ccaacttcag cctgctgaag caggccggcg acgtggagga gaaccccggc
     3781 cccatgaaga ccttcaacat ctctcagcag gaccttgagc tggtggaagt tgccactgag
     3841 aaaatcacca tgctctatga ggacaacaag caccatgttg gtgctgccat caggaccaag
     3901 acaggagaaa tcatctctgc tgtccacatt gaagcctaca ttggcagggt cactgtgtgt
     3961 gcagaggcca ttgccattgg gtctgcagtc tccaatgggc agaaggactt tgacaccatt
     4021 gtggctgtga ggcaccccta cagtgatgag gtggacaggt ccatcagagt ggtgtccccc
     4081 tgtggcatgt gcagggagct catctcagac tatgcccctg attgctttgt tctgattgag
     4141 atgaatggca agctggtcaa gacaaccatt gaggagctga ttccactgaa atacaccaga
     4201 aactaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4261 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4321 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4381 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4441 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4501 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4561 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4621 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4681 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4741 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4801 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4861 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4921 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4981 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5041 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5101 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     5161 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     5221 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5281 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     5341 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5401 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5461 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     5521 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5581 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5641 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     5701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6121 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6181 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6241 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6301 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6361 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6421 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6481 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6541 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6601 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6661 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6721 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6781 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6841 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6901 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6961 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7021 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7081 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7141 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7441 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7681 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7981 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8041 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8101 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8161 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8221 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8281 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     8341 ggaacaaaag ctggagctgc aagc
//
