LOCUS       sgYAP-R-3        8365 bp DNA     circular SYN 01-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   CSHL
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..413
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d
                     /label=ApEinfo_fwdcolor=#bc8dbf_ApEinfo_revcolor=#bc8d...
                     /note="label=""5'LTR"" - note=""ApEinfo_revcolor=#bc8dbf""
                     - note=""ApEinfo_fwdcolor=#bc8dbf"" - 1..413"
     LTR             232..412
                     /label=HIV-1 5 LTR
     misc_feature    464..601
                     /label=psi
                     /note="label=""psi"" - 464..601"
     misc_feature    523..567
                     /label=HIV-1 psi pack
     misc_feature    568..932
                     /label=gag
                     /note="label=""gag"" - 568..932"
     misc_feature    1077..1310
                     /label=RRE
     misc_feature    1078..1319
                     /label=RRE
                     /note="label=""RRE"" - 1078..1319"
     misc_feature    1694..1713
                     /label=pre-hU6_SEQ
     promoter        1717..1941
                     /label=hU6
                     /note="label=""hU6"" - 1717..1965"
     misc_feature    1717..1739
                     /label=SANGER SEQ primer
     misc_feature    1884..1924
                     /label=pool screening F_misc_feature
                     /note="note=""pool screening F"" - 1884..1924"
     misc_feature    1917..1941
                     /label=cloning_fwd_primer
     misc_feature    2422..2504
                     /label=sgRNA backbone_misc_feature
                     /note="note=""sgRNA backbone"" - 3847..3949"
     misc_feature    2422..2451
                     /label=cloning_rev_primer
     misc_feature    2546..2663
                     /label=cPPT
                     /note="label=""cPPT"" - 3991..4108"
     misc_feature    2575..2599
                     /label=pool screening R (2nd round)_misc_feature
                     /note="note=""pool screening R (2nd round)"" - 4020..4044"
     misc_feature    2585..2628
                     /label=pool screening R_misc_feature
                     /note="note=""pool screening R"" - 4030..4073"
     promoter        2707..2957
                     /label=EFS
                     /note="label=""EFS"" - 4152..4402"
     CDS             2972..2995
                     /label=Flag tag
     CDS             2999..3712
                     /label=EGFP
     misc_feature    3719..3784
                     /label=P2A
     misc_feature    3785..4207
                     /label=BlastR
     misc_binding    4222..4810
                     /label=WPRE
                     /note="bound_moiety="""" - label=""WPRE"" - 5175..5763"
     CDS             complement(4693..4704)
                     /label=FactorXa site
     LTR             4881..5116
                     /label=3' LTR SIN
                     /note="label=""3' LTR SIN"" - 5834..6069"
     misc_feature    4882..4934
                     /label=delta U3
     LTR             4935..5115
                     /label=HIV-1 5 LTR
     polyA_signal    5193..5323
                     /label=SV40 polyadenylation signal
                     /note="label=""SV40 polyadenylation signal"" - 6146..6276"
     misc_feature    5354..5431
                     /label=SV40 ORI
     rep_origin      5403..5478
                     /direction=RIGHT
                     /label=SV40 origin of replication
                     /note="label=""SV40 origin of replication"" - 6356..6431"
     primer_bind     complement(5509..5528)
                     /label=T7
     primer_bind     complement(5538..5555)
                     /label=M13-fwd
     CDS             5626..5694
                     /label=LacZ alpha
     rep_origin      5696..6151
                     /direction=RIGHT
                     /label=F1 ori
                     /note="label=""F1 ori"" - 6649..7104"
     rep_origin      complement(5701..6141)
                     /direction=LEFT
                     /label=F1 ori
     promoter        6212..6240
                     /label=Amp prom
     CDS             6282..7142
                     /label=AmpR
                     /note="label=""AmpR"" - 7235..8095"
     CDS             6480..7139
                     /label=AmpR
     rep_origin      7290..7957
                     /direction=RIGHT
                     /label=pUC ori
                     /note="label=""pUC ori"" - 8243..8910"
     rep_origin      7291..7919
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        8225..8254
                     /label=lac
     misc_binding    8259..8281
                     /label=LacO
     primer_bind     8287..8307
                     /label=M13-rev
     primer_bind     8325..8344
                     /label=T3
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggagc ctcatgggca
     1981 cggtctgttt cagagctatg cacaagagtg catagcaagt tgaaataagg ctagtccgtt
     2041 atcaactttg ctggaaacag caaagtggca ccgagtcggt gctttttttg cgtgaccgag
     2101 cttgtctgcc cgcgcagtcc actagacaga cgcgcagagg tcgggcgggc ctagggccaa
     2161 tttcccatgg ttcccttcat ttgcatatat gatgtaataa ggttatggag actattaaac
     2221 ttagccctaa tcaaactata tgatgataat gtgtgtggta caaaaggtca taacttatta
     2281 tatactttga aacttaaaaa agggttacag tttagtcacc ataactgtaa aattttttct
     2341 attcttagct ttatatagtt cttgagaggc catgtttatc gttgcggtca ggtcacgccg
     2401 cgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     2461 ctttgctgga aacagcaaag tggcaccgag tcggtgcttt ttttgaattc tagatcttga
     2521 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2581 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2641 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     2701 gccggctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 caggtgtcgt gacgcgggat ccgccaccat ggattacaaa gacgatgacg ataagatggt
     3001 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3061 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3121 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3181 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3241 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3301 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3361 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3421 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3481 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3541 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3601 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3661 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca aggctagcgg
     3721 cagcggcgcc accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg
     3781 ccccatgaag accttcaaca tctctcagca ggaccttgag ctggtggaag ttgccactga
     3841 gaaaatcacc atgctctatg aggacaacaa gcaccatgtt ggtgctgcca tcaggaccaa
     3901 gacaggagaa atcatctctg ctgtccacat tgaagcctac attggcaggg tcactgtgtg
     3961 tgcagaggcc attgccattg ggtctgcagt ctccaatggg cagaaggact ttgacaccat
     4021 tgtggctgtg aggcacccct acagtgatga ggtggacagg tccatcagag tggtgtcccc
     4081 ctgtggcatg tgcagggagc tcatctcaga ctatgcccct gattgctttg ttctgattga
     4141 gatgaatggc aagctggtca agacaaccat tgaggagctg attccactga aatacaccag
     4201 aaactaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     4261 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4321 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4381 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4441 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4501 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4561 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4621 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4681 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4741 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4801 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     4861 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4921 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5101 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     5161 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5461 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     5521 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5581 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5641 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     5701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6121 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     6181 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6241 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6301 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6361 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6421 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6481 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6541 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6601 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6661 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6721 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6781 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6841 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6901 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6961 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7021 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7081 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7141 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7201 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7621 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8041 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8101 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8161 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8221 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8281 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     8341 gggaacaaaa gctggagctg caagc
//
