LOCUS       Exported                4718 bp ds-DNA     circular SYN 04-DEC-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 4, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSR"
     promoter        complement(244..348)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        637..655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    656..680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     721..740
                     /label=ndeI
     CDS             725..1736
                     /codon_start=1
                     
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDALE
                     LLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGVED
                     VLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQELQPP
                     VQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALLLRAL
                     FNGLLQEQLAHQNQRLPELGALLKQVNHLLRAAALPGQFPLLVGYYHRELKNLILVSAG
                     LNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLMLSAE"
     CDS             1202..1736
                     /codon_start=1
                     /label=Rssb_C
                     /translation="LQPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLD
                     VTRAGHNGVLAALLLRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRAAALPGQFPL
                     LVGYYHRELKNLILVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQ
                     IWGTGGRLRLMLSAE"
     primer_bind     complement(1459..1498)
                     /label=c760g_a761c_rev
     primer_bind     1465..1502
                     /label=RssB_Q254A_N256A_For
     primer_bind     complement(1465..1502)
                     /label=RssB_Q254A_N256A_Rev
     primer_bind     complement(1717..1744)
                     /label=BamHI
     CDS             1785..1802
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1869..1916
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1953..2408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(3391..3936)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     CDS             complement(4101..4718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="VDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAIT
                     MSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMA
                     TTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGII
                     AALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
ORIGIN
        1 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
       61 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      121 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      181 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      361 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      421 gcgtatcacg aggccctttc gtcttcaaga attttataaa ccgtggagcg ggcaatactg
      481 agctgatgag caatttccgt tgcaccagtg cccttctgat gaagcgtcag cacgacgttc
      541 ctgtccacgg tacgcctgcg gccaaatttg attcctttca gctttgcttc ctgtcggccc
      601 tcattcgtgc gctctaggat ctcgatcccg cgaaattaat acgactcact ataggggaat
      661 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
      721 acatatgacg cagccattgg tcggaaaaca gattctcatt gttgaagatg agcaggtatt
      781 tcgctcgctt ctggattcat ggttttcctc attgggagcg acaacggtac tggcggctga
      841 tggggtggat gcccttgagt tgctgggagg tttcactcca gacctgatga tatgtgatat
      901 cgcgatgcca cgaatgaacg ggcttaaact gctggagcat atacgtaaca gaggcgacca
      961 gaccccagtt ctggtgatat ctgccactga aaatatggca gatattgcca aagcgttacg
     1021 tctgggcgtt gaagatgttt tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat
     1081 ggtttttgcc tgtctctatc ccagcatgtt taattcgcgc gttgaggaag aggaaaggct
     1141 ttttcgcgac tgggatgcaa tggttgataa ccctgccgca gcggcgaaat tattacagga
     1201 actacaaccg ccggttcagc aggtgatttc ccattgccgg gttaattatc gtcaattggt
     1261 tgccgcggac aaacccggcc tggtgcttga tattgccgca ctttcggaaa acgatctggc
     1321 attttattgc cttgatgtca cccgagctgg acataatggc gtacttgctg ccttgttatt
     1381 acgcgcattg tttaacggat tattacagga acagcttgca caccaaaatc aacggttgcc
     1441 agagttgggc gcgttattga agcaggtaaa ccatttactt cgtgcggccg ctctgccggg
     1501 gcagtttccg ctattagttg gctattatca tcgcgaactg aaaaatctca ttctggtttc
     1561 tgcgggtctg aatgcgacgt taaataccgg cgaacaccag gtgcaaatca gtaatggtgt
     1621 tccgttaggc actttaggta acgcttattt gaatcaattg agccagcgat gcgatgcctg
     1681 gcaatgccaa atatggggaa ccggtggtcg actgcgcttg atgttgtctg cagaatgagg
     1741 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
     1801 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1861 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     1921 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     1981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2161 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2281 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     2401 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2461 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
     2521 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     2581 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     2641 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     2701 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     2761 acccgggcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2821 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     2881 gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga
     2941 tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat
     3001 caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct
     3061 ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc
     3121 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     3181 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     3241 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     3301 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     3361 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     3421 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     3481 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     3541 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     3601 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
     3661 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
     3721 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
     3781 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
     3841 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
     3901 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taaggggtct
     3961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4201 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4381 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     4441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaac
//
