LOCUS       Exported                2741 bp ds-DNA     circular SYN 21-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Jiten Doshi
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 4, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     226..245
                     /label=PR-JD37
                     /note="pJD02/03 sequencing primer"
     misc_feature    233..304
                     /label=upstream MCS
                     /note="upstream multiple cloning site"
     primer_bind     234..260
                     /label=Forward (CW) Analysis Primer
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="Genewiz primer collection"
     misc_feature    315..318
                     /label=Restriction site
     gene            complement(319..641)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /label=E2B
                     /db_xref="GeneID:2652985"
     prim_transcript complement(319..641)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /experiment="transcript mapping"
     gene            319..641
                     /gene="L5"
                     /locus_tag="HAdVC_gp06"
                     /old_locus_tag="HAdVCgp09"
                     /label=L5
                     /db_xref="GeneID:2652999"
     gene            319..641
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /label=L4
                     /db_xref="GeneID:2653005"
     gene            319..641
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=L3
                     /db_xref="GeneID:2652998"
     gene            319..641
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=L2
                     /db_xref="GeneID:2652996"
     gene            319..641
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /label=L1
                     /db_xref="GeneID:2652993"
     prim_transcript 319..641
                     /gene="L5"
                     /locus_tag="HAdVC_gp06"
                     /old_locus_tag="HAdVCgp09"
                     /experiment="transcript mapping"
     prim_transcript 319..641
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /experiment="transcript mapping"
     prim_transcript 319..641
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /experiment="transcript mapping"
     prim_transcript 319..641
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /experiment="transcript mapping"
     prim_transcript 319..641
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /experiment="transcript mapping"
     intron          complement(319..641)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L5"
                     /locus_tag="HAdVC_gp06"
                     /old_locus_tag="HAdVCgp09"
                     /label=precedes fiber CDS
                     /note="precedes fiber CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /label=precedes capsid protein precursor pVIII CDS
                     /note="precedes capsid protein precursor pVIII CDS"
     intron          319..641
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /note="precedes protein 33K and encapsidation protein 22K 
                     CDSs"
     intron          319..641
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /label=precedes hexon assembly protein 100K CDS
                     /note="precedes hexon assembly protein 100K CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=precedes protease CDS
                     /note="precedes protease CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=precedes hexon CDS
                     /note="precedes hexon CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=precedes capsid protein precursor pVI CDS
                     /note="precedes capsid protein precursor pVI CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes core protein precursor pX CDS
                     /note="precedes core protein precursor pX CDS"
     intron          319..641
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes core protein V CDS
                     /note="precedes core protein V CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes core protein precursor pVII CDS
                     /note="precedes core protein precursor pVII CDS"
     intron          319..641
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes penton base CDS
                     /note="precedes penton base CDS"
                     /experiment="transcript mapping"
     intron          319..641
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /label=precedes capsid protein precursor pIIIa CDS
                     /note="precedes capsid protein precursor pIIIa CDS"
     intron          319..641
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /label=precedes encapsidation protein 52K CDS
                     /note="precedes encapsidation protein 52K CDS"
                     /experiment="transcript mapping"
     misc_feature    319..400
                     /label=upstream Ad sequence
     CDS             complement(319..370)
                     /codon_start=1
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /product="terminal protein precursor pTP"
                     /function="DNA replication"
                     /label=E2B
                     /note="covalently attached to genome 5'-termini; 
                     genus-common"
                     /db_xref="GI:160409919"
                     /db_xref="GeneID:2652985"
                     /protein_id="NP_040518.2"
                     /translation="SVNDCARLTGQSVPTME"
     misc_feature    401..556
                     /label=VA I RNA
     misc_feature    432..531
                     /label=truncated-va
                     /note="Crystal structure of an adenovirus virus-associated 
                     RNA, 2019, Nature CommWorks as efficiently as wild-type in 
                     inhibiting PKR gene"
     misc_feature    455..467
                     /label=b box
                     /note="/Kamel et al 2013 The adenovirus VA RNA-derived 
                     miRNAs are not essential for lytic virus growth in tissue 
                     culture cells"
     misc_feature    557..563
                     /label=termination signal?
                     /note="/Wang et al 2000 Nuclear factor 1 (NF1) affects 
                     accurate termination and multiple-round transcription by 
                     human RNA polymerase III"
     misc_feature    596..604
                     /label=termination signal??
                     /note="/Literature suggests a stretch of T residues are the
                     termination signal for RNA pol III/Wang et al 2000 Nuclear 
                     factor 1 (NF1) affects accurate termination and 
                     multiple-round transcription by human RNA polymerase III"
     misc_feature    650..692
                     /label=downstream MCS
                     /note="downstream multiple cloning site"
     primer_bind     complement(697..722)
                     /label=Reverse (CCW) Analysis Primer
     primer_bind     complement(703..722)
                     /label=T7
                     /note="Genewiz primer collection"
     promoter        complement(704..722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(779..789)
                     /label=PR-JD31
                     /note="pJD11 Ef1a-rtTA sequencing primer"
     promoter        814..918
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(862..881)
                     /label=PR-JD31
                     /note="pJD11 Ef1a-rtTA sequencing primer"
     CDS             919..1779
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1137..1156)
                     /label=Amp-R
                     /note="Quinatarabio5' end of ampiciltrn resistance gene, 
                     reverse primer"
     misc_feature    1780..1863
                     /label=terminator sequence?
     primer_bind     complement(1851..1869)
                     /label=PR-JD02
                     /note="RP to amplify gene fragment JD1-01 for AmpR gene 
                     cloning in golden gate backbone; dig by BanI, BsaAI"
     promoter        complement(1864..1876)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1941..1959)
                     /label=PR-JD32
                     /note="pJD02/03 sequencing primer"
     rep_origin      1977..2565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2501..2520
                     /label=PR-JD29
                     /note="pJD11 rtTA CDS, pA sequencing primer"
     primer_bind     2675..2693
                     /label=PR-JD28
                     /note="pJD10 tTA CDS sequencing primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggaggc tccatggtcg ggacgctctg gccggtgagg cgtgcgcagt
      361 cgttgacgct ctagaccgtg caaaaggaga gcctgtaagc gggcactctt ccgtggtctg
      421 gtggataaat tcgcaagggt atcatggcgg acgaccgggg ttcgaacccc ggatccggcc
      481 gtccgccgtg atccatgcgg ttaccgcccg cgtgtcgaac ccaggtgtgc gacgtcagac
      541 aacgggggag cgctcctttt ggcttccttc caggcgcggc ggctgctgcg ctagcttttt
      601 tggccactgg ccgcgcgcgg cgtaagcggt taggctggaa accattcctg tagacttctt
      661 aattaagacg tcagaattct cgaggcggcc gcatgtgagt ctccctatag tgagtcgtat
      721 taatttcgcg ggcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      781 gctcatgagt agcaccaggc gtttaagggc acccgcggaa cccctatttg tttatttttc
      841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1621 gccggtgagc gtggatctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1741 atcgctgaga taggtgcgtc actgattaag cattggtaac tgtcagacca agtttactca
     1801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1861 ctttacgtgc cgatcaaagt ctcattttcg ccaaaagttg tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaatgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag c
//
