LOCUS       Exported                2656 bp ds-DNA     circular SYN 03-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Jiten Doshi
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 4, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     226..245
                     /label=PR-JD37
                     /note="pJD02/03 sequencing primer"
     misc_feature    233..304
                     /label=upstream MCS
                     /note="upstream multiple cloning site"
     primer_bind     234..260
                     /label=Forward (CW) Analysis Primer
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="Genewiz primer collection"
     primer_bind     315..338
                     /label=Primer 1
     gene            complement(318..516)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /label=E2B
                     /db_xref="GeneID:2652985"
     prim_transcript complement(318..516)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /experiment="transcript mapping"
     gene            318..516
                     /gene="L5"
                     /locus_tag="HAdVC_gp06"
                     /old_locus_tag="HAdVCgp09"
                     /label=L5
                     /db_xref="GeneID:2652999"
     gene            318..516
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /label=L4
                     /db_xref="GeneID:2653005"
     gene            318..516
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=L3
                     /db_xref="GeneID:2652998"
     gene            318..516
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=L2
                     /db_xref="GeneID:2652996"
     gene            318..516
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /label=L1
                     /db_xref="GeneID:2652993"
     prim_transcript 318..516
                     /gene="L5"
                     /locus_tag="HAdVC_gp06"
                     /old_locus_tag="HAdVCgp09"
                     /experiment="transcript mapping"
     prim_transcript 318..516
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /experiment="transcript mapping"
     prim_transcript 318..516
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /experiment="transcript mapping"
     prim_transcript 318..516
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /experiment="transcript mapping"
     prim_transcript 318..516
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /experiment="transcript mapping"
     intron          complement(318..516)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L5"
                     /locus_tag="HAdVC_gp06"
                     /old_locus_tag="HAdVCgp09"
                     /label=precedes fiber CDS
                     /note="precedes fiber CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /label=precedes capsid protein precursor pVIII CDS
                     /note="precedes capsid protein precursor pVIII CDS"
     intron          318..516
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /note="precedes protein 33K and encapsidation protein 22K 
                     CDSs"
     intron          318..516
                     /gene="L4"
                     /locus_tag="HAdVC_gp07"
                     /label=precedes hexon assembly protein 100K CDS
                     /note="precedes hexon assembly protein 100K CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=precedes protease CDS
                     /note="precedes protease CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=precedes hexon CDS
                     /note="precedes hexon CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L3"
                     /locus_tag="HAdVC_gp08"
                     /label=precedes capsid protein precursor pVI CDS
                     /note="precedes capsid protein precursor pVI CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes core protein precursor pX CDS
                     /note="precedes core protein precursor pX CDS"
     intron          318..516
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes core protein V CDS
                     /note="precedes core protein V CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes core protein precursor pVII CDS
                     /note="precedes core protein precursor pVII CDS"
     intron          318..516
                     /gene="L2"
                     /locus_tag="HAdVC_gp09"
                     /label=precedes penton base CDS
                     /note="precedes penton base CDS"
                     /experiment="transcript mapping"
     intron          318..516
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /label=precedes capsid protein precursor pIIIa CDS
                     /note="precedes capsid protein precursor pIIIa CDS"
     intron          318..516
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /label=precedes encapsidation protein 52K CDS
                     /note="precedes encapsidation protein 52K CDS"
                     /experiment="transcript mapping"
     intron          complement(318..516)
                     /gene="E2B"
                     /locus_tag="HAdVC_gp04"
                     /old_locus_tag="HAdVCgp06"
     misc_RNA        347..504
                     /gene="VAII"
                     /locus_tag="HAdVC_gs02"
                     /old_locus_tag="HAdVCgs02"
                     /product="virus-associated RNA II"
                     /db_xref="GeneID:2653002"
                     /experiment="transcript mapping"
     misc_feature    347..503
                     /label=VA II RNA Ad strain C
     misc_feature    398..412
                     /label=b box va ii rna
                     /note="/Kamel et al 2013 The adenovirus VA RNA-derived 
                     miRNAs are not essential for lytic virus growth in tissue 
                     culture cells"
     misc_feature    504..511
                     /label=termination region
                     /note="/Literature suggests a stretch of T residues are the
                     termination signal for RNA pol III"
     CDS             521..546
                     /codon_start=1
                     /gene="L1"
                     /locus_tag="HAdVC_gp10"
                     /old_locus_tag="HAdVCgp12"
                     /product="encapsidation protein 52K"
                     /function="DNA encapsidation;capsid morphogenesis"
                     /label=L1
                     /note="genus-common"
                     /db_xref="GI:9626171"
                     /db_xref="GeneID:2652993"
                     /protein_id="NP_040519.1"
                     /translation="MHPVLRQM"
     primer_bind     complement(539..560)
                     /label=Primer 2
     misc_feature    555..556
                     /label=downstream MCS
                     /note="downstream multiple cloning site"
     misc_feature    565..607
                     /label=downstream MCS
                     /note="downstream multiple cloning site"
     primer_bind     complement(612..637)
                     /label=Reverse (CCW) Analysis Primer
     primer_bind     complement(618..637)
                     /label=T7
                     /note="Genewiz primer collection"
     promoter        complement(619..637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(694..704)
                     /label=PR-JD31
                     /note="pJD11 Ef1a-rtTA sequencing primer"
     promoter        729..833
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(777..796)
                     /label=PR-JD31
                     /note="pJD11 Ef1a-rtTA sequencing primer"
     CDS             834..1694
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1695..1778
                     /label=terminator sequence?
     primer_bind     complement(1766..1784)
                     /label=PR-JD02
                     /note="RP to amplify gene fragment JD1-01 for AmpR gene 
                     cloning in golden gate backbone; dig by BanI, BsaAI"
     promoter        complement(1779..1791)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1856..1874)
                     /label=PR-JD32
                     /note="pJD02/03 sequencing primer"
     rep_origin      1892..2480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2416..2435
                     /label=PR-JD29
                     /note="pJD11 rtTA CDS, pA sequencing primer"
     primer_bind     2590..2608
                     /label=PR-JD28
                     /note="pJD10 tTA CDS sequencing primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggaggt taggctggaa agcgaaagca ttaagtggct cgctccctgt
      361 agccggaggg ttattttcca agggttgagt cgcaggaccc ccggttcgag tctcgggccg
      421 gccggactgc ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc ttgcaaattc
      481 ctccggaaac agggacgagc cccttttttg cttttcccag atgcatccgg tgctgcggca
      541 gatgcgccat tcctgtccat tcctgtagac ttcttaatta agacgtcaga attctcgagg
      601 cggccgcatg tgagtctccc tatagtgagt cgtattaatt tcgcgggcgg aacccctatt
      661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagtagcac caggcgttta
      721 agggcacccg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      841 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      901 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      961 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1021 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1081 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1141 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1201 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1261 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1321 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1381 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1441 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1501 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta
     1561 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1621 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcgtcactga
     1681 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1741 ttcattttta atttaaaagg atctaggtga agatccttta cgtgccgatc aaagtctcat
     1801 tttcgccaaa agttgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2461 gggggcggag cctatggaaa aacgccagca atgcggcctt tttacggttc ctggcctttt
     2521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2641 cagtgagcga ggaagc
//
