LOCUS       Exported               11496 bp ds-DNA     circular SYN 28-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 3, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
                     /note="color: #66cc99; direction: RIGHT"
     primer_bind     2060..2081
                     /label=QB2741
                     /note="color: black; sequence: tgcaggggaaagaatagtagac"
     primer_bind     2163..2203
                     /label=Vector_bGH_F
                     /note="color: black; sequence: 
                     AGCAGAGATCCAGTTTATCGATCCATAGAGCCCACCGCATC"
     misc_feature    complement(2185..2412)
                     /label=bGH PolyA Terminator
                     /note="color: #3366ff; direction: LEFT"
     primer_bind     complement(2392..2422)
                     /label=bGH_mCherry_R
                     /note="color: black; sequence: 
                     ccgcgtcgacCGACTGTGCCTTCTAGTTGCC"
     primer_bind     complement(2396..2418)
                     /label=Balanced_pLV_Bidi_mCherry_Cterm_OPEN_R
                     /note="color: black; sequence: gtcgacCGACTGTGCCTTCTAGT"
     primer_bind     2404..2451
                     /label=PasteBack_ pLV_Bidi_Add_dsDomain_cterm_mCherry_F
                     /note="color: black; sequence: 
                     GCACAGTCGgtcgacgcggccgctctacacattgatcctagcagaagc"
     primer_bind     complement(2404..2427)
                     /label=pLV_Bidi_mCherry_Cterm_OPEN_R
                     /note="color: black; sequence: agcggccgcgtcgacCGACTGTGC"
     primer_bind     2413..2451
                     /label=pLV_Bidi_Add_dsDomain_cterm_mCherry_F
                     /note="color: black; sequence: 
                     gtcgacgcggccgctctacacattgatcctagcagaagc"
     misc_feature    2413..2427
                     /label=OldVector
                     /note="color: #a6acb3"
     primer_bind     2427..2451
                     /label=dsGFP_MluI_R
                     /note="color: black; sequence: 
                     cgaggaattcacgcgtctacacattgatcctagcagaagc"
     CDS             complement(2428..2556)
                     /codon_start=1
                     /label=destabilization domain
                     /note="color: #ffff00"
                     /translation="KLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     primer_bind     complement(2536..2571)
                     /label=pLV_Bidi_Add_dsDomain_cterm_mCherry_R
                     /note="color: black; sequence: 
                     gacgagctgtacaagaagcttagccatggcttcccg"
     CDS             complement(2557..3264)
                     /codon_start=1
                     /label=mCherry
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2557..2578
                     /label=pLV_Bidi_mCherry_Cterm_OPEN_F
                     /note="color: black; sequence: cttgtacagctcgtccatgccg"
     primer_bind     2742..2761
                     /label=QB1639
                     /note="color: black; sequence: cgtccttcagcttcagcctc"
     primer_bind     complement(2818..2835)
                     /label=QB1485
                     /note="color: black; sequence: aagaccatgggctgggag"
     misc_feature    complement(3274..3784)
                     /label=hPGK_Promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(3338..3354)
                     /label=QB4277
                     /note="color: black; sequence: tgttccgcattctgcaa"
     primer_bind     complement(3777..3822)
                     /label=hPGK_Vector_R
                     /note="color: black; sequence: 
                     aagacgaaagggcctcatcgatgatatcgaattcccacggggttgg"
     misc_feature    3785..3800
                     /label=OldVector
                     /note="color: #a6acb3"
     promoter        3831..5002
                     /label=EF-1a
                     /note="/vntifkey=29"
                     /note="color: #99ccff; direction: RIGHT"
     primer_bind     complement(3894..3914)
                     /label=QB2749
                     /note="color: black; sequence: tctaggcaccggttcaattgc"
     primer_bind     complement(4041..4060)
                     /label=QB1690
                     /note="color: black; sequence: acttacctgtgttctggcgg"
     primer_bind     complement(4767..4786)
                     /label=QB0500
                     /note="color: black; sequence: ctaatcgaggtgcctggacg"
     primer_bind     5025..5065
                     /label=EF1a_h5UTRMYC_MluI_F
                     /note="color: black; sequence: 
                     tagatcgcgaacgcgtaactcgctgtagtaattccagcgag"
     misc_feature    5041..5448
                     /label=h5'UTR_MYC
                     /note="color: #00ffff"
     CDS             5449..6294
                     /codon_start=1
                     /label=dsGFP
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(6144..6165)
                     /label=pLV_Bidi_mCherry_Cterm_OPEN_F
                     /note="color: black; sequence: cttgtacagctcgtccatgccg"
     primer_bind     6151..6186
                     /label=pLV_Bidi_Add_dsDomain_cterm_mCherry_R
                     /note="color: black; sequence: 
                     gacgagctgtacaagaagcttagccatggcttcccg"
     primer_bind     complement(6271..6333)
                     /label=PasteBack_ pLV_Bidi_Add_dsDomain_cterm_mCherry_F
                     /note="color: black; sequence: 
                     GCACAGTCGgtcgacgcggccgctctacacattgatcctagcagaagc"
     primer_bind     complement(6271..6333)
                     /label=pLV_Bidi_Add_dsDomain_cterm_mCherry_F
                     /note="color: black; sequence: 
                     gtcgacgcggccgctctacacattgatcctagcagaagc"
     primer_bind     complement(6271..6310)
                     /label=dsGFP_MluI_R
                     /note="color: black; sequence: 
                     cgaggaattcacgcgtctacacattgatcctagcagaagc"
     misc_feature    6307..6965
                     /label=IRES
                     /note="/vntifkey=21"
                     /note="color: #ff99cc; direction: RIGHT"
     primer_bind     6564..6583
                     /label=QB1559
                     /note="color: black; sequence: ccaaaggaatgcaaggtctg"
     primer_bind     6785..6804
                     /label=QB0037
                     /note="color: black; sequence: tggctctcctcaagcgtatt"
     CDS             6966..7394
                     /codon_start=1
                     /label=BlastR
                     /note="/vntifkey=4"
                     /note="color: #ff9966"
                     /translation="MVMKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEI
                     ISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGM
                     CRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     misc_feature    7418..8009
                     /label=WPRE
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     primer_bind     complement(7471..7491)
                     /label=QB0112
                     /note="color: black; sequence: catagcgtaaaaggagcaaca"
     primer_bind     7895..7912
                     /label=QB5834
                     /note="color: black; sequence: ctcaatccagcggacctt"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatccatag agcccaccgc atccccagca tgcctgctat
     2221 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     2281 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     2341 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     2401 aaggcacagt cggtcgacgc ggccgctcta cacattgatc ctagcagaag cacaggctgc
     2461 agggtgacgg tccatcccgc tctcctgggc acaagacatg ggcagcgtgc catcatcctg
     2521 ctcctccacc tccggcggga agccatggct aagcttcttg tacagctcgt ccatgccgcc
     2581 ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg
     2641 ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt
     2701 ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct
     2761 ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc
     2821 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
     2881 cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac
     2941 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
     3001 caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg
     3061 aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc
     3121 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
     3181 gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt
     3241 atcctcctcg cccttgctca ccatcggatc cccctgggga gagaggtcgg tgattcggtc
     3301 aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc
     3361 gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg
     3421 gccgctgctc tcggcgcgcc ccgctgagca gccgctattg gccacagccc atcgcggtcg
     3481 gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc
     3541 ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg
     3601 agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg
     3661 aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg
     3721 cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa
     3781 ccccgtggca attcgatatc atcgatgagg ccctttcgtc ttcactcgag gtgcccgtca
     3841 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3901 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     3961 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4021 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4081 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4141 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4201 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4261 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4321 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4381 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     4441 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     4501 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     4561 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     4621 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     4681 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     4741 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     4801 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     4861 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     4921 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     4981 tcttccattt caggtgtcgt gaggatccgc ggccgcgccg gctctagatc gcgaacgcgt
     5041 aactcgctgt agtaattcca gcgagaggca gagggagcga gcgggcggcc ggctagggtg
     5101 gaagagccgg gcgagcagag ctgcgctgcg ggcgtcctgg gaagggagat ccggagcgaa
     5161 tagggggctt cgcctctggc ccagccctcc cgctgatccc ccagccagcg gtccgcaacc
     5221 cttgccgcat ccacgaaact ttgcccatag cagcgggcgg gcactttgca ctggaactta
     5281 caacacccga gcaaggacgc gactctcccg acgcggggag gctattctgc ccatttgggg
     5341 acacttcccc gccgctgcca ggacccgctt ctctgaaagg ctctccttgc agctgcttag
     5401 acgctggatt tttttcgggt agtggaaaac cagcagcctc ccgcgacgat ggtgagcaag
     5461 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5521 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5581 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5641 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5701 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5761 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5821 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5881 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5941 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     6001 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     6061 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6121 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct tagccatggc
     6181 ttcccgccgg aggtggagga gcaggatgat ggcacgctgc ccatgtcttg tgcccaggag
     6241 agcgggatgg accgtcaccc tgcagcctgt gcttctgcta ggatcaatgt gtagacgcgt
     6301 gaattcctcg agggcggccg ctctagagtc gacgggccgc ggtaacaatt gttaactaac
     6361 ttaagctagc aacggtttcc ctctagcggg atcaattccg cccccccccc ctaacgttac
     6421 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6481 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6541 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6601 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6661 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6721 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6781 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6841 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6901 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata
     6961 ataccatggt catgaaaaca tttaacattt ctcaacaaga tctagaatta gtagaagtag
     7021 cgacagagaa gattacaatg ctttatgagg ataataaaca tcatgtggga gcggcaattc
     7081 gtacgaaaac aggagaaatc atttcggcag tacatattga agcgtatata ggacgagtaa
     7141 ctgtttgtgc agaagccatt gcgattggta gtgcagtttc gaatggacaa aaggattttg
     7201 acacgattgt agctgttaga cacccttatt ctgacgaagt agatagaagt attcgagtgg
     7261 taagtccttg tggtatgtgt agggagttga tttcagacta tgcaccagat tgttttgtgt
     7321 taatagaaat gaatggcaag ttagtcaaaa ctacgattga agaactcatt ccactcaaat
     7381 atacccgaaa ttaacccggg cggggcgcgt ctggaacaat caacctctgg attacaaaat
     7441 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7501 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7561 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7621 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7681 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7741 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7801 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7861 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7921 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7981 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     8041 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     8101 gcacaagagg aggaggaggt gggtttttcc agtcacacct caggtacctt taagaccaat
     8161 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     8221 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     8281 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     8341 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     8401 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     8461 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     8521 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     8581 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     8641 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8701 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8761 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8821 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8881 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta
     8941 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9001 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9061 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9121 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9781 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9901 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11101 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    11161 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11221 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11281 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11341 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    11401 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa
    11461 ttgtatttgt taaatatgta ctacaaactt agtagt
//
