LOCUS       pGL3-SV40Promote        5391 bp DNA     circular SYN 24-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..249
                     /label=SV40 Promoter
     misc_feature    95..172
                     /label=SV40 Origin
     misc_feature    251..658
                     /label=h5'UTR_MYC
     primer_bind     complement(609..645)
                     /label=pGL3_Mutagenesis_5UTRMyc_COMP_PntMutInDel_363_R
     misc_feature    613..644
                     /label=Del 363-394
     primer_bind     631..662
                     /label=pGL3_Mutagenesis_5UTRMyc_COMP_PntMutInDel_363_F
     misc_feature    661..2592
                     /label=F-Luc
     misc_feature    2345..2566
                     /label=SV40 late poly(A) signal
     misc_feature    3653..4513
                     /label=Ampicillin
     misc_feature    4555..4583
                     /label=AmpR promoter
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt aactcgctgt agtaattcca gcgagaggca gagggagcga gcgggcggcc
      301 ggctagggtg gaagagccgg gcgagcagag ctgcgctgcg ggcgtcctgg gaagggagat
      361 ccggagcgaa tagggggctt cgcctctggc ccagccctcc cgctgatccc ccagccagcg
      421 gtccgcaacc cttgccgcat ccacgaaact ttgcccatag cagcgggcgg gcactttgca
      481 ctggaactta caacacccga gcaaggacgc gactctcccg acgcggggag gctattctgc
      541 ccatttgggg acacttcccc gccgctgcca ggacccgctt ctctgaaagg ctctccttgc
      601 agctgcttag accgaccatt aaaatggggt agtcgttttg gtcgagcctc ccgcgacgcc
      661 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
      721 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      781 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      841 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      901 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      961 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     1021 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     1081 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     1141 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     1201 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     1261 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     1321 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     1381 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     1441 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     1501 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     1561 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     1621 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1681 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1741 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1801 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1861 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1921 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1981 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     2041 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     2101 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     2161 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     2221 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     2281 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt
     2341 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     2401 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2461 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2521 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     2581 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2641 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2701 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2761 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2821 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2881 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2941 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3001 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3061 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3121 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3181 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3241 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3301 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3361 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3421 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3481 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3541 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3601 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3661 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3721 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3781 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3841 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3901 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3961 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4021 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4081 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4141 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4201 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4261 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4321 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4381 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4441 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4501 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4561 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4621 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4681 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4741 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4801 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4861 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4921 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4981 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5041 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5101 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5161 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
     5221 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     5281 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
     5341 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat a
//
