LOCUS       pBHM2641_|_NeoR_-_osTI 11436 bp ds-DNA     circular     05-NOV-2024
DEFINITION  .
COMMENT     Cut pRS316 backbone with BamHI, HindIII PCR nat marker PCR degron
            transform in yeast
FEATURES             Location/Qualifiers
     promoter        190..410
                     /label="URA3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             411..1214
                     /label="URA3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(1343..1798)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     1943..1959
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     promoter        1966..1984
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2808..3602
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(2862..2881)
                     /label="Neo-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     3472..3491
                     /label="Neo-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3809..4531
                     /label="TEF1 promoter sequence"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3958..3979)
                     /label="pRS316-pTEF1-CAS9_opt 2166-2187 REV 1"
                     /note="sequence: gggtgtgatacttgacataccg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4532..5030
                     /label="5'UTR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4652..5019
                     /label="tef1 5'utr intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4775..4796)
                     /label="pRS316-pTEF1-CAS9_opt 2983-3004 REV 2"
                     /note="sequence: acaagacagaatgatgtgggtg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             join(5031..5474,5527..7740)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKILRSGSGEGRGSLLTCGDVEENPGPMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYKGSGSGGSPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK*"
     misc_feature    5031..6807
                     /label="Cno_OsTIR1(F74G)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5475..5526
                     /label="05429_intron_opt"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             6823..6876
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from Thosea asigna virus capsid protein protein_id: transl_table: 1 translation: EGRGSLLTCGDVEENPGP"
     misc_feature    6877..7584
                     /label="mNeonGreen"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7132..7141
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    7585..7605
                     /label="linker"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    7606..7737
                     /label="AID*"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    7741..8613
                     /label="tef1 term"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(8003..8024)
                     /label="pRS316-pTEF1-CAS9_opt 7750-7771 REV 8"
                     /note="sequence: aagcagcaacgaaggttattcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(8662..8680)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(8701..8717)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    8725..8741
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(8749..8755)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(8756..8773)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(8774..8779)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(9103..9691)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(9862..10653)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(10654..10722)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(10723..10827)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    10864..11367
                     /label="CEN/ARS"
                     /ApEinfo_revcolor="#454f59"
                     /ApEinfo_fwdcolor="#454f59"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCACGCTTT TCAATTCAAT TCATCATTTT TTTTTTATTC TTTTTTTTGA TTTCGGTTTC
      241 TTTGAAATTT TTTTGATTCG GTAATCTCCG AACAGAAGGA AGAACGAAGG AAGGAGCACA
      301 GACTTAGATT GGTATATATA CGCATATGTA GTGTTGAAGA AACATGAAAT TGCCCAGTAT
      361 TCTTAACCCA ACTGCACAGA ACAAAAACCT GCAGGAAACG AAGATAAATC ATGTCGAAAG
      421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
      481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
      541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
      601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
      661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
      721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
      781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
      841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
      901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
      961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
     1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
     1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
     1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
     1201 GCCAGCAAAA CTAAAAAACT GTATTATAAG TAAATGCATG TATACTAAAC TCACAAATTA
     1261 GAGCTTCAAT TTAATTATAT CAGTTATTAC CCTGCGGTGT GAAATACCGC ACAGATGCGT
     1321 AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA
     1381 AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
     1441 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA
     1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
     1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
     1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
     1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
     1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG
     1801 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
     1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
     1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA
     1981 TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC GGCCGCTCTA GAACTAGTgc atgcaggatt
     2041 cgagtggcat ggtgtgcact gagtgtatgg ttgtcggagg agaggatgat ggtaacaaca
     2101 atagcagcaa cgtcactcga cgcgcgtccg gtgtgccaca cggggtaacg ccgagtcgcc
     2161 gtcagggtcg ccgagaccac tctcacagcg tcaccgttgg caccagctca gcttacagct
     2221 tctatcctcc gccagcatcc acatacatcc cctataccgc atcccccacc cactgcccaa
     2281 ggtgagtcat cttcccgccc ccttcccttg cccgccactc agtcctccat cctccactaa
     2341 tccaccttat cgcacccacc gcctatcgca catccgagca caatgctggg cctgccaggg
     2401 gctgctagat ggtgctctcc ccacgctgat ctgcatgccg gccattggat catgggtgct
     2461 aggtgctggg tgctggatgt tggatgctgg atgctgggtg cacgcttggt catttccttc
     2521 caggattgac ggtcgccgag aggacgacgt ggcgttcgac aacgagggcc gatagcaccg
     2581 catcgcctcg acctgcatcc atctcgcctt gtccttttgg tgcaacaatc catccgtgct
     2641 ggtgccacac gcatagctgg aagagatgga tgtgcgttga acagagctgc cgtcaggact
     2701 ttttggtgca cggaccctat tgtcctcccc aatcttcacc gcgtctccta atatgcagcc
     2761 tctttgctaa ttgtcttttt ccattagtaa actcgcccaa catgtctatg attgaacaag
     2821 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     2881 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     2941 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3001 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3061 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3121 cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3181 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3241 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3301 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3361 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3421 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3481 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     3541 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     3601 gagtgaaggc ggtaaggggt taattttcct tagagggtgt atatatacat attagagaag
     3661 tgatacaatt ttagcacact gcgaattcga gacagacatc gtgtcaatca tcttttttga
     3721 cattatatgc ccattaatct atctacagac aacaatacca tccttcccac cctcagcaac
     3781 gccgttgaat cctcaggatc ttcatggcca ttctatgcta tacggtacaa gattgtggct
     3841 actatgcaag ctccatagcg aatgatctta cactgacctt gtcgaccgtt ctgaatgatt
     3901 aacaatggtc gcaagtgtgg ttgacccacc tgcgggcgct tcttaactat ctatcatcgg
     3961 tatgtcaagt atcacaccct ttaaacaaaa gaataagagc ataatacagc agtctcatca
     4021 tggcaaccct tcgcgcaatg tacaaaaagc agccagaagc ccccacgcac aagcaataat
     4081 gtcgtgttct accctgccat gaacaagcgt catggccatt agcgaacgcc ccaatattca
     4141 gccccattcg ccaagcaagt tgcctcgccc ccttcccaga agatcatggg catctcgtct
     4201 tcttctaatt tcgcattttg tgaagctcca ttcatgcacg tcggcttctg gacgactctt
     4261 atcatatgca ttggcctgct cggtgtgagg atgagccact cccacatgtt tagacggcag
     4321 gggcctcttg ccacccgttg aatttttggt gcgaagacgt gcctgggaac gacctccact
     4381 ttaaatttct ccgttgttgc ggtcgctcca tcctcctcac atttcttgcc tcctaagtcc
     4441 catgtgccac actcggtgaa aaacggagtt tgatacggat gaaatttcca gtgagctttt
     4501 cacattctgg aggtttcgaa aaaaattgtg ggattttctg aattaatccg agaatccgcc
     4561 ctgcccatca accggaaaga attaaatccc tcgcacattc ctttctccca ccaccttctt
     4621 tatctctaca ttctttaacc aaaacaccta ggtgagtacc caaagtcttt tgacaccttt
     4681 gaaaatccgc gccagtcgta gggcacgtaa aacaattctc gctggtggct tctgcaggtg
     4741 aagtcggggc agagtggagc tgagcatggt cgaccaccca catcattctg tcttgttttt
     4801 ggcgatgtct tccatgcttt tgccctcatc tgttgcgtcg actgcagagt actgttttgc
     4861 cactactgag ctcaacatgg gcttgtgtgt ggtgaagaaa aaaatttggt ggctcttgaa
     4921 aattttcaac ccatcggata catgcccttc caactcacaa cgccctaaca ctttattgtc
     4981 ctatcattgc ctcttcgcac atgctaacga tctccacaga aaacttcaaa atgacctact
     5041 tccccgagga ggtcgtcgag cacatcttct ccttcctccc cgcccagcga gaccgaaaca
     5101 ccgtctccct cgtctgtaag gtctggtacg agatcgagcg actctcccga cgaggtgtct
     5161 tcgtcggtaa ctgttacgcc gtccgagccg gtcgagtcgc cgcccgattc cccaacgtcc
     5221 gagccctcac cgtcaagggt aagccccacg gtgccgactt caacctcgtc ccccccgact
     5281 ggggtggtta cgccggtccc tggatcgagg ccgccgcccg aggttgtcac ggtctcgagg
     5341 agctccgaat gaagcgaatg gtcgtctccg acgagtccct cgagctcctc gcccgatcct
     5401 tcccccgatt ccgagccctc gtcctcatct cctgtgaggg tttctccacc gacggtctcg
     5461 ccgccgtcgc ctccgtatgt aaccccgttt cccgcaacat gttactccac taacatctcc
     5521 cgacagcact gtaagctcct ccgagagctc gacctccagg agaacgaggt cgaggaccga
     5581 ggtccccgat ggctctcctg tttccccgac tcctgtacct ccctcgtctc cctcaacttc
     5641 gcctgtatca agggtgaggt caacgccggt tccctcgagc gactcgtctc ccgatccccc
     5701 aacctccgat ccctccgact caaccgatcc gtctccgtcg acaccctcgc caagatcctc
     5761 ctccgaaccc ccaacctcga ggacctcggt accggtaacc tcaccgacga cttccagacc
     5821 gagtcctact tcaagctcac ctccgccctc gagaagtgta agatgctccg atccctctcc
     5881 ggtttctggg acgcctcccc cgtctgtctc tccttcatct accccctctg tgcccagctc
     5941 accggtctca acctctccta cgcccccacc ctcgacgcct ccgacctcac caagatgatc
     6001 tcccgatgtg tcaagctcca gcgactctgg gtcctcgact gtatctccga caagggtctc
     6061 caggtcgtcg cctcctcctg taaggacctc caggagctcc gagtcttccc ctccgacttc
     6121 tacgtcgccg gttactccgc cgtcaccgag gagggtctcg tcgccgtctc cctcggttgt
     6181 cccaagctca actccctcct ctacttctgt caccagatga ccaacgccgc cctcgtcacc
     6241 gtcgccaaga actgtcccaa cttcacccga ttccgactct gtatcctcga gcccggtaag
     6301 cccgacgtcg tcacctccca gcccctcgac gagggtttcg gtgccatcgt ccgagagtgt
     6361 aagggtctcc agcgactctc catctccggt ctcctcaccg acaaggtctt catgtacatc
     6421 ggtaagtacg ccaagcagct cgagatgctc tccatcgcct tcgccggtga ctccgacaag
     6481 ggtatgatgc acgtcatgaa cggttgtaag aacctccgaa agctcgagat ccgagactcc
     6541 cccttcggtg acgccgccct cctcggtaac ttcgcccgat acgagaccat gcgatccctc
     6601 tggatgtcct cctgtaacgt caccctcaag ggttgtcagg tcctcgcctc caagatgccc
     6661 atgctcaacg tcgaggtcat caacgagcga gacggttcca acgagatgga ggagaaccac
     6721 ggtgacctcc ccaaggtcga gaagctctac gtctaccgaa ccaccgccgg tgcccgagac
     6781 gacgccccca acttcgtcaa gatcctcaga tcaggaagcg gaGAGGGTCG AGGTTCCCTC
     6841 CTCACCTGTG GTGACGTCGA GGAGAACCCC GGTCCCATGG TCTCCAAGGG TGAGGAGGAC
     6901 AACATGGCCT CCCTCCCCGC CACCCACGAG CTCCACATCT TCGGTTCCAT CAACGGTGTC
     6961 GACTTCGACA TGGTCGGTCA GGGTACCGGT AACCCCAACG ACGGTTACGA GGAGCTCAAC
     7021 CTCAAGTCCA CCAAGGGTGA CCTCCAGTTC TCCCCCTGGA TCCTCGTCCC CCACATCGGT
     7081 TACGGTTTCC ACCAGTACCT CCCCTACCCC GACGGTATGT CCCCCTTCCA GGCCGCCATG
     7141 GTCGACGGTT CCGGTTACCA GGTCCACCGA ACCATGCAGT TCGAGGACGG TGCCTCCCTC
     7201 ACCGTCAACT ACCGATACAC CTACGAGGGT TCCCACATCA AGGGTGAGGC CCAGGTCAAG
     7261 GGTACCGGTT TCCCCGCCGA CGGTCCCGTC ATGACCAACT CCCTCACCGC CGCCGACTGG
     7321 TGTCGATCCA AGAAGACCTA CCCCAACGAC AAGACCATCA TCTCCACCTT CAAGTGGTCC
     7381 TACACCACCG GTAACGGTAA GCGATACCGA TCCACCGCCC GAACCACCTA CACCTTCGCC
     7441 AAGCCCATGG CCGCCAACTA CCTCAAGAAC CAGCCCATGT ACGTCTTCCG AAAGACCGAG
     7501 CTCAAGCACT CCAAGACCGA GCTCAACTTC AAGGAGTGGC AGAAGGCCTT CACTGACGTC
     7561 ATGGGTATGG ACGAGCTCTA CAAGGGTTCT GGATCCGGAG GTAGTcctaa agatccagcc
     7621 aaacctccgg ccaaggcaca agttgtggga tggccaccgg tgagatcata ccggaagaac
     7681 gtgatggttt cctgccaaaa atcaagcggt ggcccggagg cggcggcgtt cgtgaagtaa
     7741 ttacttatgt agtgaccctt cttacgcgta atatgcatcg gatgactgtt gctacttgtg
     7801 cttcttacgt tttgtgtctt atttgctgat gtatacctct gcgcttcaaa atcttcattg
     7861 ccgtgatata tcattccatg tcgtttgtcc gttattggat tcccgatagt tgtccgcgaa
     7921 atcgaatatt tctcggctga cgacgtctct tgcaacaata aaatggccat cactgttgaa
     7981 atttgcacga gagctataac ttggaataac cttcgttgct gctttatttt agtgctttca
     8041 actatttact cattctcagg tcgccaccat cgcattgata tcgttccttc catccttcct
     8101 ttcctttagt cggtcacgtc ttccttaatc cgtcactcct tttttcagtc gttcctggag
     8161 atcaccacga aataatctcg acatgcactg accatctcgg ttctgtggac tgtcatgcta
     8221 agagagttca tgttgtaata atagtttgtt gcatggaaag aacggggaaa gaagcaagaa
     8281 acagatgtac agaattaccg atgtctctac gcatccctga cctcgtcctt cctaccgcaa
     8341 ccgcacttcc acctgctcac acgtcctcgt taatgccttg aatctcagcc acacacttac
     8401 ctcgcctcga cgcttgtcgg gccagggcac ccccttcatc aacctgggaa gagttggcgg
     8461 ggcaaacgga ggagggatgg ggcaggaaga gggggatggc gcacgtcgag tggatttgga
     8521 agaaaagggg ttggtcgagg tggaagtcga agcgaagatg ttgaatgtca tgtttgggaa
     8581 cgggggatct ttagaagact atgagattga acgATCGATA CCGTCGACCT CGAGGGGGGG
     8641 CCCGGTACCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT CCGAGCTTGG CGTAATCATG
     8701 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATAGGAGC
     8761 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGGTAACTCA CATTAATTGC
     8821 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
     8881 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
     8941 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     9001 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     9061 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCGGCCC
     9121 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     9181 ATAAAGATAC CAGGCGTTCC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     9241 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG
     9301 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     9361 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     9421 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     9481 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     9541 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     9601 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     9661 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     9721 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     9781 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     9841 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     9901 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTGCCCGTC GTGTAGATAA CTACGATACG
     9961 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
    10021 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
    10081 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
    10141 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
    10201 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
    10261 CCCCATGTTG TGAAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
    10321 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
    10381 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
    10441 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
    10501 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
    10561 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
    10621 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
    10681 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
    10741 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
    10801 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGGGTCCTT
    10861 TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT ATATAAATTA
    10921 AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC GAAGATGTAA
    10981 AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC TGCTCTCAGG
    11041 TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA AATCCTGTGA
    11101 TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT TTCTTGTCTA
    11161 ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG TTTATTTTTT
    11221 TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC AAATACAAAA
    11281 CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA AAGATACGAG
    11341 GCGCGTGTAA GTTACAGGCA AGCGATCCGT CCTAAGAAAC CATTATTATC ATGACATTAA
    11401 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC
//