LOCUS       Exported                4726 bp ds-DNA     circular SYN 01-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     YSP             1..33
                     /label=Mutation
     extracted       1..33
                     /label=Extracted region from Y54-C1 pDR2x-mVenus(L46F; 
                     T63S)-P2A-PAmCherry-T2A-mtagBFP1
     restriction     1..33
                     /label=Bsp1407I
     misc_feature    1..17
                     /label=纯去除mito后
     primer_bind     17..53
                     /label=mNG-F
     primer_bind     17..38
                     /label=42-F
     primer_bind     17..37
                     /label=49-F
     primer_bind     complement(17..33)
                     /label=mgold载体反向
     primer_bind     28..53
                     /label=0719-PM948-F
     regulatory      28..33
                     /label=vertebrate consensus sequence for strong initia
                     /label=vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)
     CDS             34..741
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      34..37
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      289..298
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(721..756)
                     /label=mNG-R
     primer_bind     complement(721..741)
                     /label=0719-PM948-R
     primer_bind     complement(725..756)
                     /label=42-R
     primer_bind     742..763
                     /label=mgold载体正向
     primer_bind     complement(742..756)
                     /label=OXstaygold-R
     CDS             742..744
                     /label=mGold CDS
     CDS             742..744
                     /label=mVenus
     CDS             742..744
                     /label=Translation 1435-1437
     polyA_signal    867..988
                     /label=SV40 poly(A) signal
     primer_bind     complement(904..923)
                     /label=SV40pA-R
     primer_bind     958..977
                     /label=EBV-rev
     primer_bind     complement(965..988)
                     /label=SV40-POLYA-R
     rep_origin      complement(995..1450)
                     /direction=LEFT
                     /label=f1 ori
     primer_bind     complement(1132..1153)
                     /label=F1ori-F
     primer_bind     1344..1363
                     /label=F1ori-R
     promoter        1477..1581
                     /label=AmpR promoter
     promoter        1583..1940
                     /label=SV40 promoter
     primer_bind     complement(1606..1626)
                     /label=pBABE 3'
     rep_origin      1791..1926
                     /label=SV40 ori
     primer_bind     1853..1872
                     /label=SV40pro-F
     CDS             1975..2769
                     /label=NeoR/KanR
     primer_bind     complement(2029..2048)
                     /label=Neo-R
     primer_bind     2639..2658
                     /label=Neo-F
     primer_bind     2750..2771
                     /label=KanR-F
     primer_bind     complement(2750..2771)
                     /label=KanR-R
     primer_bind     complement(2957..2976)
                     /label=TK-pA-R
     polyA_signal    3001..3048
                     /label=HSV TK poly(A) signal
     rep_origin      3377..3965
                     /label=ori
     primer_bind     3866..3885
                     /label=pBR322ori-F
     YSP             4067..4675
                     /label=Mutation
     YSP             4067..4675
                     /label=YSP021 T63S mutation
     YSP             4067..4675
                     /label=YSP10,11
     enhancer        4140..4443
                     /label=CMV enhancer
     promoter        4444..4647
                     /label=CMV promoter
     primer_bind     4598..4618
                     /label=CMV-F
     primer_bind     4598..4618
                     /label=CMV-F
     primer_bind     complement(4658..4675)
                     /label=载体.反向
     misc_feature    4659..4675
                     /label=mito前
     primer_bind     4661..4690
                     /label=片段.正向
     CDS             4676..4726
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 atccattcgt tgggggatcc accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggcctctc tcccagcgac acatgagtta cacatctttg gctccatcaa cggtgtggac
      121 tttgacatgg tgggtcaggg caccggcaat ccaaatgatg gttatgagga gttaaacctg
      181 aagtccacca agggtgacct ccagttctcc ccctggattc tggtccctca tatcgggtat
      241 ggcttccatc agtacctgcc ctaccctgac gggatgtcgc ctttccaggc cgccatggta
      301 gatggctccg gctaccaagt ccatcgcaca atgcagtttg aagatggtgc ctcccttact
      361 gttaactacc gctacaccta cgagggaagc cacatcaaag gagaggccca ggtgaagggg
      421 actggtttcc ctgctgacgg tcctgtgatg accaactcgc tgaccgctgc ggactggtgc
      481 aggtcgaaga agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac
      541 accacgggaa atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag
      601 ccaatggcgg ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc
      661 aagcactcca agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg
      721 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
      781 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
      901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      961 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     1021 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     1081 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     1141 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     1201 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     1261 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     1321 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     1381 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     1441 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1561 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     1621 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1681 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     1741 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     1801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1921 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     1981 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     2041 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     2101 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     2161 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     2221 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     2281 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     2341 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     2401 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     2461 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     2521 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     2581 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     2641 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     2701 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     2761 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     2821 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     2881 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     2941 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     3001 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     3061 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     3121 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     3181 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     3241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     4081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4441 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4501 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4561 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4621 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcatggg
     4681 cgtggccgac ctgatcaaga agttcgagag catcagcaag gaggag
//
