LOCUS       pAAV.RMacL5ET-09.EGFP.BCB8.WPRE.dna        6466 bp    DNA     circular UNA 10-FEB-2025
DEFINITION  .
ACCESSION   urn.local...1p-iss7hkv
VERSION     urn.local...1p-iss7hkv
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          1..6466
     misc_feature    1..130
                     /note="/application_notes=Allows rescue of virus from
                     recombinant plasmid and replication of the viral genome;
                     this ITR is identical to that of the wild-type AAV2
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat
                     (functional equivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
                     /standard_name="5' ITR"
     regulatory      join(158..658,659..686,687..1553)
                     /note="Geneious type: promoter"
                     /note="/application_notes="
                     /note="/official_designation={L3PN.Enh003.Hsp68}"
                     /note="/uuid=5c7c5a2e7c89453487ba7ceeedf3163f"
                     /standard_name="{L5ET.Enh009.Hsp68}"
     misc_feature    1578..1583
                     /note="/application_notes=Facilitates translation
                     initiation of ATG start codon downstream of the Kozak
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /standard_name="Kozak"
     CDS             1584..2303
                     /note="/application_notes=Commonly used green fluorescent
                     protein; ranked high in brightness, photostability and pH
                     stability among all fluorescent proteins."
                     /note="/description=Enhanced green fluorescent protein;
                     codon optimized based on a variant of wild type GFP from
                     the jellyfish Aequorea victoria"
                     /note="/full_name=EGFP reporter gene"
                     /note="/official_designation=EGFP"
                     /note="/uuid=0470849bb38e4f4b8a1fe5d436422af3"
                     /standard_name="EGFP"
     regulatory      2335..2844
                     /note="Geneious type: misc_signal"
                     /standard_name="BC.B8"
     misc_feature    2875..3472
                     /note="/application_notes=Enhances virus stability in
                     packaging cells, leading to higher titer of packaged
                     virus; enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /standard_name="WPRE"
     misc_feature    3503..3710
                     /note="Geneious type: polyA_signal"
                     /note="/application_notes=Allows transcription termination
                     and polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /standard_name="BGH pA"
     misc_feature    complement(3718..3847)
                     /note="/application_notes=Allows rescue of virus from
                     recombinant plasmid and replication of the viral genome;
                     this ITR is identical to that of the wild-type AAV2
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a5d684b85c014f76a7b6d8e97945f0fb"
                     /standard_name="3' ITR"
     CDS             4775..5635
                     /note="/application_notes=Allows E. coli to be resistant
                     to ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /standard_name="AmpiciIIin"
     rep_origin      5806..6394
ORIGIN      
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgtga ttatctgggt ggaccatcac
      181 aagtgtcctt gcgcaagcga ggcaaacgga gattagacgc acagaaagag agaaggcaat
      241 gtgaccacca aggcagaaat tagaataata cggccacatg tcaaggaatg cctgcagctg
      301 caggagctgg aagggacaag aagcagattt tccctagagc cttccaaagg cccacagccc
      361 tgctgacagc tcgcttccac ccagtgatac agatttcaga cttctggctt ccagaacagt
      421 gagggaatat atttctgttg ttttaaacca tctagtttat ggtaatttgt tactgccgtc
      481 acaggaaacg aatacagaat atgaagaaga tgtgaaagta ttcatacctt taaaacaagg
      541 cagaaagaag gaagcaagga tgctgagctc atgaatgcaa tgttagtcac tcagcacaca
      601 aaggagtgaa agccaaataa actaatccag aaaaccatgt gtcttttgaa aataaatctc
      661 ctagtttcca tgctctagcc tgcaggcagg aacatccaaa ctgagcagcc ggggtccccc
      721 ccacccccca ccccgcccca cgcggcaact ttgagcctgt gctgggacag agcctctagt
      781 tcctaaatta gtccatgagg tcagaggcag cactgccatt gtaacgcgat tggagaggat
      841 cacgtcaccg gacacgcccc caggcatctc cctgggtctc ctaaacttgg cggggagaag
      901 ttttagccct taagttttag cctttaaccc ccatattcag aactgtgcga gttggcgaaa
      961 ccccacaaat cacaacaaac tgtacacaac accgagctag aggtgatctt tcttgtccat
     1021 tccacacagg ccttagtaat gcgtcgccat agcaacagtg tcactagtag caccagcact
     1081 tccccacacc ctccccctca ggaatccgta ctctccagtg aaccccagaa acctctggag
     1141 agttctggac aagggcggaa cccacaactc cgattactca agggaggcgg ggaagctcca
     1201 ccagacgcga aactgctgga agattcctgg ccccaaggcc tcctccggct cgctgattgg
     1261 cccagcggag agtgggcggg gccggtgaag actccttaaa ggcgcagggc ggcgagcagg
     1321 tcaccagacg ctgacagcta ctcagaacca aatctggttc catccagaga caagcgaaga
     1381 caagagaagc agagcgagcg gcgcgttccc gatcctcggc caggaccagc cttccccaga
     1441 gcatccctgc cgcggagcgc aaccttccca ggagcatccc tgccgcggag cgcaactttc
     1501 cccggagcat ccacgccgcg gagcgcagcc ttccagaagc agagcgcggc gcccaagttt
     1561 gtacaaaaaa gcaggctgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1741 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1801 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1981 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2041 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2101 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2161 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     2221 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2281 gcatggacga gctgtacaag taagtttaaa cacagggtta atcattaact acagctaccg
     2341 agtgatccag tccgcataag ataccacaca ttgcgaaggt taccattgac cttgtctccg
     2401 atattcctta tctgaggaat tacctgtcct gcaatgagac cgttagcgta ctcgctgtca
     2461 taggagccgc tctgaaccac cggagccggt actaggttgg aagagaccat acgtctgact
     2521 cgttgtgtgt ctcgctcagg aaccacttca ggattatgga ctactcatct gtcggtcaag
     2581 gataagttcg acctgaagtt agaaccaagt agccacggtc acaggttaag attcagtgta
     2641 tcctcgcaga ccttcgcaac cagacttgag ttcttatcca cgagtgtgta gtgccattct
     2701 acactacagc ggtcaacaag gtgccctgtc ttaattggtt gcgcgtgttg actccaattc
     2761 acgttcgcca ccgttatctc tgtgagcgag aactgccata gtgcctatgg tcagttagca
     2821 accgcacgcg ttatgattgc ttccacccag ctttcttgta caaagtggga attccgataa
     2881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3241 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     3301 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattcct
     3481 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3541 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3601 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3661 caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga gggccgcagg
     3721 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3781 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3841 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3901 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4141 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4261 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4381 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4501 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4561 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5461 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
     5521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6421 gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcag
//
