LOCUS       Exported                8194 bp ds-DNA     circular SYN 07-NOV-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Stephen Graham
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 7, 2024 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     626..645
                     /label=T7 fwd
                     /note="color: black; sequence: taatacgactcactataggg; added:
                     2020-08-04"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     repeat_region   708..942
                     /label=PB 3' TR
                     /note="color: #a6acb3"
     promoter        1145..1536
                     /label=Second generation TRE promoter
                     /note="color: #ffffff"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     primer_bind     1501..1520
                     /label=PB-T fwd
                     /note="color: black; sequence: CCATAGAAGACACCGGGACC; added:
                     2020-01-01"
     regulatory      1567..1576
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             1573..1620
                     /codon_start=1
                     /label=OROV_Gn (Signal sequence)
                     /label=ORF1
                     /note="color: #993366"
                     /translation="MANLIIISMVLGVAYG"
     CDS             1627..2865
                     /codon_start=1
                     /label=OROV Gc
                     /note="color: #993366"
                     /translation="DEDCLSKDIKITYQELHNCIGPKIMGDTCVSKSELYSDLFSKNLV
                     TEYDKKYFEPDTVNDQFNKIEFAQDAHRMILLERILYKTECEMLSLKKNSGPYNVAWRT
                     YFKNHNIDLCSRHNYKMICQCINTHSMCKNTDIDYNKEIETYYKSNAAAYRADLNTIMD
                     TLKTAFRGLTKVLIENYIEKDDSDALKALFSNITDSVQDNYQMIGILKFASKLLDINLG
                     RSTRSAHHSIMTNEIPKSNPFTDYSYSNLNIKECMSPESLKCFKKRDSTPHTNHLLCKI
                     DNKYKAFDWPEIETIQKGQKLCLGDSHCNLEFTAITADKIMSLTNCYKESFTAQPADMQ
                     AGIKKCSADEIGECTTLEDKTWPIIFCGGKYYYSDSKEHAKDGSINNYCLTNKCSEQRF
                     PIHENWFKKCNWDK"
     CDS             2863..2883
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=LysHis6
                     /note="color: #cc99b2"
                     /translation="KHHHHHH"
     misc_feature    2917..3505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #a6acb3"
     primer_bind     complement(2970..2990)
                     /label=WPRE-R
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA; 
                     added: 2020-01-01"
     CDS             complement(3388..3399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3387"
                     /translation="IEGR"
     primer_bind     complement(3599..3616)
                     /label=BGH rev
                     /note="color: black; sequence: tagaaggcacagtcgagg; added: 
                     2020-08-04"
     polyA_signal    3605..3829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    4113..4160
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: RIGHT"
     rep_origin      4254..4389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             4393..4995
                     /label=Puromycin resistance marker
                     /note="color: #993366; direction: RIGHT"
     polyA_signal    5229..5350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   5546..5858
                     /label=PB 5' TR
                     /note="color: #a6acb3"
     promoter        complement(6003..6021)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6042..6058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6066..6082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6090..6120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6090 .. 6096 / #ffffff / -10
                        2: 6097 .. 6114 / #ffffff
                        3: 6115 .. 6120 / #ffffff / -35"
     protein_bind    6135..6156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6444..7032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7203..8063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7203 .. 7994 / #ccffcc
                        2: 7995 .. 8063 / #ccffcc / signal sequence
                     Cleavage site after base 7994"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8064..8168)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 gctagcgcca ccatggccaa cctgatcatc atcagcatgg tgctgggcgt cgcctacggc
     1621 ggatccgatg aggactgcct gagcaaggac atcaagatca cctaccaaga gctgcacaac
     1681 tgcatcggcc ccaagatcat gggcgatacc tgcgtgtcca agagcgagct gtactccgac
     1741 ctgttcagca agaacctggt caccgagtac gacaagaagt actttgagcc cgacaccgtg
     1801 aacgaccagt tcaacaagat cgagttcgcc caggacgccc accggatgat cctgctggaa
     1861 agaatcctgt acaagaccga gtgcgagatg ctgagcctga agaagaacag cggcccctac
     1921 aatgtggcct ggcggaccta cttcaagaac cacaacatcg acctgtgcag ccggcacaac
     1981 tacaagatga tctgccagtg catcaacacc cacagcatgt gcaagaacac cgacatcgac
     2041 tacaacaaag agatcgaaac ctactacaag agcaacgccg ctgcctacag agccgacctg
     2101 aacaccatca tggacaccct gaaaaccgcc ttccggggcc tgacaaaggt gctgatcgag
     2161 aactacatcg agaaggacga cagcgacgcc ctgaaggccc tgttctccaa catcaccgac
     2221 agcgtgcagg acaattacca gatgatcggc atcctgaagt tcgccagcaa gctgctggac
     2281 atcaacctgg gcagaagcac cagaagcgcc caccacagca tcatgaccaa cgagatcccc
     2341 aagagcaatc ccttcaccga ctacagctac agcaacctga acatcaaaga atgcatgagc
     2401 cccgagtctc tgaagtgctt caagaagcgg gacagcaccc ctcacaccaa ccatctgctg
     2461 tgcaagatcg acaacaagta caaggccttc gactggcccg agatcgagac aatccagaaa
     2521 ggccagaagc tgtgcctggg cgacagccac tgcaaccttg agtttaccgc catcaccgcc
     2581 gataagatca tgagcctgac caactgctac aaagagagct tcacagccca gcctgccgat
     2641 atgcaggccg gcatcaagaa gtgtagcgcc gacgagatcg gcgagtgcac cacactggaa
     2701 gataagacct ggcctatcat cttctgcggc ggcaagtact actacagcga ctccaaagag
     2761 cacgccaagg acggcagcat caacaactac tgtctgacca acaagtgcag cgagcagcgg
     2821 ttccccatcc acgagaactg gtttaagaag tgcaactggg acaagcatca ccaccatcat
     2881 cactgactta agggtaccga attcgatatc aagcttaatc aacctctgga ttacaaaatt
     2941 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3001 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3061 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3121 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3181 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3241 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3301 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3361 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3421 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3481 atctcccttt gggccgcctc cccgcgcggc cgcatagata actgatccag tgtgctgggc
     3541 tacgatgcat gggcccccta ggtcgagtct agagggcccg tttaaacccg ctgatcagcc
     3601 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3661 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3721 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3781 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3841 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4081 aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct
     4141 ctagaaagta taggaacttc cttggccaaa aagaccggta ccaggcaggc agaagtatgc
     4201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4321 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4381 ttggaggcta ccatgggtac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4441 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4501 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4561 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4621 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4681 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4741 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4801 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4861 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4921 ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc
     4981 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     5041 ccccatggct ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga
     5101 ggcccaccga ctctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     5161 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5221 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5281 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5341 atgtatctta acgcgtcgag tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5401 ttatcatgtc tgtataccgt cgacctctag ctgcaggaat tcgatatcaa gctctagcca
     5461 acaagctcgt catcgctttg cagaagagca gagaggatat gctcatcgtc taaagaacta
     5521 cccattttat tatatattag tcacgatatc tataacaaga aaatatatat ataataagtt
     5581 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     5641 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     5701 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     5761 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     5821 tgcgtcaatt ttacgcagac tatctttcta gggttaaaaa agatttgcgc tttactcgac
     5881 ctaaacttta aacacgtcat agaatcttcg tttgacaaaa accacattgt ggccaagctg
     5941 tgtgacgcga cgcgcgctaa agaatggcaa accaagtcgc gcgaggtacc cagcttttgt
     6001 tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6061 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6181 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6241 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6301 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6361 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8161 gttccgcgca catttccccg aaaagtgcca cctg
//
