LOCUS       Exported               12631 bp ds-DNA     circular SYN 03-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     exon            1349..1520
                     /label=exon1
     exon            1604..1740
                     /label=exon2
     exon            1823..1996
                     /label=exon3
     exon            2086..2322
                     /label=exon4
     exon            2402..2502
                     /label=exon5
     exon            2578..2650
                     /label=exon6
     exon            2737..2805
                     /label=exon7
     exon            2897..3004
                     /label=exon8
     exon            3096..3221
                     /label=exon9
     exon            3299..3475
                     /label=exon10
     protein_bind    complement(3502..3526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3552..3804
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3838..3854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3862..3878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3886..3916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3931..3952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3988..4240
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4391..5416)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5468..5647)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6153..6177
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6698..7489
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7735..8323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8509..8649
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8993..9187
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9253..10326)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10755..11384)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagcaggc tccgaattcg cccttaagga agcgggatcg gtttacgtcg atgtagctgg
     1381 ggaatcgaag tcggagcatg ttactgggaa gtggttttcg gtgccggagc tccgactgcg
     1441 tgaccaccgg ttcatagttc ctcttgatta ttccaaaagt tctcccaaaa ttaccgtctt
     1501 tgctcgagag atcgttgcgg gtaacttgaa atttggagct ttttgattca gagctctttc
     1561 tgtgattacg aagctaaaag tttgtgtctt ttgttttttg tagttgggaa agaggagcaa
     1621 gcaatgccgt atttattgta tttgcaaggt ggtccaggat ttgaaggtcc aaggcctagt
     1681 gaagctagtg gatggattca aagagcttgt gaagagtttc gagtggtctt actcgatcaa
     1741 gtatgatgct ttcatagttt tatactcttc tctggacagt ttttttagtc tttgtgttgt
     1801 tctaattttg tttgtcctgc agcgaggaac aggcttgtcc actcctttaa catgttcctc
     1861 tatgcttcaa ttcaaatcag caaaggaatt agctgattat ttggttcact ttcgagctga
     1921 taatatagtt aaagatgctg agtttattag agttcgtctt gttcctaagg ctgatccttg
     1981 gacaattttg ggtcaggtaa tttgtctttg tattgtctat tggtcatctt gcttcagttc
     2041 ttgtcatttg attttgtatt catctttttt tcttcaaatc tacagagttt cggtggcttt
     2101 tgtgcactta catatctgag ttttgcaccc gaaggactca aacaagtgct aataactgga
     2161 ggaattccac caattggtaa agcatgtaca gcagatgatg tatatgaagc cggttttgaa
     2221 caagttgctc gccaaaatga aaaatactac aagagattcc ctcaagacat tgagattgtt
     2281 cgtgaactcg taaattactt ggctgaatct gaaggtggcg gggtgaggga ttacaacatt
     2341 tgctcaatcc actgaccagt ttagtttagt tccttacttg tctctgtttc tttgtcttta
     2401 ggtacctctc ccatcaggag gcattcttac tcctaagggt cttcaaactc ttggtctttc
     2461 tggtttagga tcaagtactg gttttgagcg cctgcattat atgtaagagt ttagcttaat
     2521 ttttatcaac cacctcacat gttctgtgtt aaatctggcc ttcttaattg atcataggtt
     2581 ggagagagta tgggatccta ttttagttac tggagctcca aagtgtatta gtcagttctt
     2641 cttaaacgct gtaagagacc cacaaaaatt tgttgttttc tgttctcagc tcaaataagg
     2701 aattcaatag tctgagacat tttatcgttg ctgcagtttg agagttggca ttcttttgat
     2761 acaaatccac tatatgctct tctccacgag gccatatact gtgaggtata gtctcttcaa
     2821 atttctcatc tttttttcta tttcagacaa ttacttcttc ttgttatcgt ttatgtttaa
     2881 atggaaactt tttcagggtg cttcatcagg atggtctgct catagattac gggataaata
     2941 tgagtacaaa ttcgacgcaa tgaaggcagt caaagaaagt cagcctgtac tcttcacagg
     3001 agaggtacag gttttgagta tttctaattg tatggctcat agcttggatg attggtccac
     3061 attgcttaac taaaatagaa tttggatttg tatagatgat atttccatgg atgtttgatg
     3121 aaattcatgc cttgaagcca tttaaagctg ccgccgatct tttggccaag aaagaggatt
     3181 ggcctccatt atatgatgtt cctagattgc agaataacaa ggtctgtttc agaatcttga
     3241 tcgttctttc atatggatca aataatttta ctctgtttca catgatttct ttttgtaggt
     3301 gcctgttgct gcagcggttt actacgaaga tatgtatgta aacttcaagc tggtgacgga
     3361 aacagcttct catatatcgg gtatcagact ttgggtaacg aatgagttta tgcattcggg
     3421 tcttcgtgat gctggaagac aaattattga tcatttgttg ggaatgatca atgggtgagg
     3481 atccatggaa gggcgaattc gacccagctt tcttgtacaa agtggtgata acctagagct
     3541 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     3601 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     3661 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     3721 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     3781 cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt aatcatggtc
     3841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat
     3961 taggcgcgcc tatttctatt aattcccgat ctagtaacat agatgacacc gcgcgcgata
     4021 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
     4081 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
     4141 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
     4201 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc gggggatcga
     4261 tcccgcgaac tgtggacgag aactgtgcca ccaagcgtaa ggccgttctc tcgcatttgc
     4321 cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg aaatcagctc ttgttcggtc
     4381 ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     4441 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     4501 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     4561 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     4621 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     4681 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     4741 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     4801 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     4861 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     4921 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     4981 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     5041 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     5101 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     5161 tctcgctgaa tgccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     5221 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     5281 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     5341 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     5401 gttcaggctt tttcatatct tattgccccc cgggatcaga tctggattga gagtgaatat
     5461 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     5521 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     5581 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt
     5641 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     5701 cgtgactccc ttaattctcc gctcatgatc ttgatcccct gcgccatcag atccttggcg
     5761 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     5821 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
     5881 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
     5941 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
     6001 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct gatccgtcga
     6061 gattaataga aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc
     6121 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     6181 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     6241 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     6301 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     6361 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     6421 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6481 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6541 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6601 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6661 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     6721 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6781 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6841 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6901 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6961 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     7021 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     7081 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     7141 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     7201 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     7261 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     7321 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     7381 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     7441 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     7501 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     7561 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     7621 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     7681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7861 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8521 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8581 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     8641 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8701 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8761 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     8821 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     8881 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     8941 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     9001 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     9061 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     9121 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     9181 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     9241 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     9301 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     9361 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     9421 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     9481 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     9541 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     9601 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     9661 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     9721 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     9781 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     9841 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     9901 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     9961 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
    10021 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
    10081 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
    10141 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
    10201 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
    10261 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
    10321 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
    10381 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
    10441 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
    10501 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
    10561 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
    10621 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
    10681 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
    10741 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
    10801 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
    10861 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
    10921 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
    10981 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
    11041 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
    11101 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
    11161 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
    11221 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
    11281 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
    11341 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
    11401 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
    11461 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    11521 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    11581 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    11641 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    11701 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    11761 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    11821 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    11881 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    11941 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    12001 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    12061 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    12121 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    12181 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    12241 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    12301 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    12361 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    12421 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    12481 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    12541 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
    12601 tgcacataca aatggacgaa cggataaacc t
//
