LOCUS       Exported               12639 bp ds-DNA     circular SYN 03-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     exon            1346..1520
                     /label=exon1
     exon            1604..1740
                     /label=exon2
     exon            1823..1996
                     /label=exon3
     exon            2086..2322
                     /label=exon4
     exon            2402..2502
                     /label=exon5
     exon            2578..2650
                     /label=exon6
     variation       2633..2635
                     /label=Q345R
     exon            2737..2805
                     /label=exon7
     exon            2897..3004
                     /label=exon8
     exon            3096..3221
                     /label=exon9
     exon            3299..3490
                     /label=exon10
     protein_bind    complement(3510..3534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3560..3812
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3846..3862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3870..3886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3894..3924)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3939..3960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3996..4248
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4399..5424)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5476..5655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6161..6185
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6706..7497
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7743..8331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8517..8657
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      9001..9195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9261..10334)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10763..11392)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagcaggc tccgaattcg cccttatgga agcgggatcg gtttacgtcg atgtagctgg
     1381 ggaatcgaag tcggagcatg ttactgggaa gtggttttcg gtgccggagc tccgactgcg
     1441 tgaccaccgg ttcatagttc ctcttgatta ttccaaaagt tctcccaaaa ttaccgtctt
     1501 tgctcgagag atcgttgcgg gtaacttgaa atttggagct ttttgattca gagctctttc
     1561 tgtgattacg aagctaaaag tttgtgtctt ttgttttttg tagttgggaa agaggagcaa
     1621 gcaatgccgt atttattgta tttgcaaggt ggtccaggat ttgaaggtcc aaggcctagt
     1681 gaagctagtg gatggattca aagagcttgt gaagagtttc gagtggtctt actcgatcaa
     1741 gtatgatgct ttcatagttt tatactcttc tctggacagt ttttttagtc tttgtgttgt
     1801 tctaattttg tttgtcctgc agcgaggaac aggcttgtcc actcctttaa catgttcctc
     1861 tatgcttcaa ttcaaatcag caaaggaatt agctgattat ttggttcact ttcgagctga
     1921 taatatagtt aaagatgctg agtttattag agttcgtctt gttcctaagg ctgatccttg
     1981 gacaattttg ggtcaggtaa tttgtctttg tattgtctat tggtcatctt gcttcagttc
     2041 ttgtcatttg attttgtatt catctttttt tcttcaaatc tacagagttt cggtggcttt
     2101 tgtgcactta catatctgag ttttgcaccc gaaggactca aacaagtgct aataactgga
     2161 ggaattccac caattggtaa agcatgtaca gcagatgatg tatatgaagc cggttttgaa
     2221 caagttgctc gccaaaatga aaaatactac aagagattcc ctcaagacat tgagattgtt
     2281 cgtgaactcg taaattactt ggctgaatct gaaggtggcg gggtgaggga ttacaacatt
     2341 tgctcaatcc actgaccagt ttagtttagt tccttacttg tctctgtttc tttgtcttta
     2401 ggtacctctc ccatcaggag gcattcttac tcctaagggt cttcaaactc ttggtctttc
     2461 tggtttagga tcaagtactg gttttgagcg cctgcattat atgtaagagt ttagcttaat
     2521 ttttatcaac cacctcacat gttctgtgtt aaatctggcc ttcttaattg atcataggtt
     2581 ggagagagta tgggatccta ttttagttac tggagctcca aagtgtatta gtcggttctt
     2641 cttaaacgct gtaagagacc cacaaaaatt tgttgttttc tgttctcagc tcaaataagg
     2701 aattcaatag tctgagacat tttatcgttg ctgcagtttg agagttggca ttcttttgat
     2761 acaaatccac tatatgctct tctccacgag gccatatact gtgaggtata gtctcttcaa
     2821 atttctcatc tttttttcta tttcagacaa ttacttcttc ttgttatcgt ttatgtttaa
     2881 atggaaactt tttcagggtg cttcatcagg atggtctgct catagattac gggataaata
     2941 tgagtacaaa ttcgacgcaa tgaaggcagt caaagaaagt cagcctgtac tcttcacagg
     3001 agaggtacag gttttgagta tttctaattg tatggctcat agcttggatg attggtccac
     3061 attgcttaac taaaatagaa tttggatttg tatagatgat atttccatgg atgtttgatg
     3121 aaattcatgc cttgaagcca tttaaagctg ccgccgatct tttggccaag aaagaggatt
     3181 ggcctccatt atatgatgtt cctagattgc agaataacaa ggtctgtttc agaatcttga
     3241 tcgttctttc atatggatca aataatttta ctctgtttca catgatttct ttttgtaggt
     3301 gcctgttgct gcagcggttt actacgaaga tatgtatgta aacttcaagc tggtgacgga
     3361 aacagcttct catatatcgg gtatcagact ttgggtaacg aatgagttta tgcattcggg
     3421 tcttcgtgat gctggaagac aaattattga tcatttgttg ggaatgatca atgggaaaaa
     3481 gcctctcttt tgaccgaatt cctatctaga cccagctttc ttgtacaaag tggtgataac
     3541 ctagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     3601 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     3661 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     3721 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     3781 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa
     3841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3901 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3961 ggctgaatta ggcgcgccta tttctattaa ttcccgatct agtaacatag atgacaccgc
     4021 gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta
     4081 taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt
     4141 aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc
     4201 aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcgg
     4261 gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc aagcgtaagg ccgttctctc
     4321 gcatttgcct tgctaggctc gcgcgagttg ctggctgagg cgttctcgaa atcagctctt
     4381 gttcggtcgg catctactct attcctttgc cctcggacga gtgctggggc gtcggtttcc
     4441 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     4501 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     4561 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     4621 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
     4681 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     4741 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     4801 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     4861 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     4921 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     4981 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     5041 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     5101 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     5161 ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     5221 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     5281 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     5341 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     5401 cgcggtgagt tcaggctttt tcatatctta ttgccccccg ggatcagatc tggattgaga
     5461 gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt
     5521 ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat
     5581 ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct
     5641 tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg
     5701 ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc gccatcagat
     5761 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     5821 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     5881 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     5941 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     6001 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga
     6061 tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa
     6121 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     6181 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     6241 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     6301 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     6361 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     6421 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     6481 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     6541 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     6601 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     6661 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     6721 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     6781 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     6841 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     6901 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     6961 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7021 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7081 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7141 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7201 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7261 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     7321 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     7381 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     7441 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     7501 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     7561 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     7621 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     7681 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7741 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7801 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7861 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7921 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7981 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8041 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8101 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8161 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8221 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8281 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8341 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8401 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8461 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     8521 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     8581 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     8641 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8701 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8761 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     8821 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     8881 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     8941 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     9001 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     9061 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     9121 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     9181 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     9241 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     9301 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     9361 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     9421 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     9481 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
     9541 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
     9601 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
     9661 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
     9721 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
     9781 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
     9841 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
     9901 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
     9961 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    10021 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    10081 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    10141 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    10201 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    10261 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    10321 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    10381 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    10441 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    10501 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    10561 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    10621 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    10681 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    10741 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    10801 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    10861 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    10921 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    10981 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    11041 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    11101 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    11161 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    11221 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    11281 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    11341 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    11401 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    11461 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    11521 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    11581 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    11641 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    11701 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    11761 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    11821 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    11881 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    11941 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    12001 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    12061 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    12121 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    12181 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    12241 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    12301 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    12361 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    12421 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    12481 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    12541 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    12601 ggttggcatg cacatacaaa tggacgaacg gataaacct
//
