LOCUS       Exported               12544 bp ds-DNA     circular SYN 03-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1219..1235
                     /label=attL1
     exon            1253..1424
                     /label=exon1
     exon            1508..1581
                     /label=exon2
     exon            1582..1644
                     /label=exon2
     exon            1727..1900
                     /label=exon3
     variation       1754..1756
                     /label=T165I
     exon            1990..2226
                     /label=exon4
     misc_feature    1990..1992
                     /label=S
     exon            2306..2406
                     /label=exon5
     misc_feature    2482..2554
                     /label=exon6
     exon            2641..2709
                     /label=exon7
     variation       2665..2667
                     /label=T359A
     exon            2801..2908
                     /label=exon8
     exon            3000..3125
                     /label=exon9
     exon            3203..3394
                     /label=exon10
     misc_feature    3233..3235
                     /label=D
     misc_feature    3317..3319
                     /label=H
     misc_feature    3400..3408
                     /label=pJLsmart_for
                     /note="pJLsmart is a entry vector to the Invitrogen GATEWAY
                     system. It is a derivate of pENTR1A (Invitrogen), in which 
                     the polylinker and ccdB gene have been repalced by a 
                     minimal MCS to allow for smart cloning using SmaI and SrfI.
                     Flanking EcoRI sites for easy clone identification and 
                     BamHI and HindIII sites for asymmetric cuts were also 
                     introduced."
     CDS             3414..3424
                     /codon_start=1
                     /label=Gateway uni-frame
                     /translation="DPA"
     protein_bind    complement(3415..3439)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(3415..3424)
                     /label=attL2
     misc_feature    complement(3418..3424)
                     /label=G-4001
                     /note="pJLsmart attL2_seq_primer"
     terminator      3465..3717
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3751..3767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3775..3791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3799..3829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3844..3865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3901..4153)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4304..5329)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5381..5560)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6066..6090
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6611..7402
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7648..8236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8422..8562
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8906..9100
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9166..10239)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10668..11297)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc aggctccgaa ttcgccctta aggaagcggg
     1261 atcggtttac gtcgatgtag ctggggaatc gaagtcggag catgttactg ggaagtggtt
     1321 ttcggtgccg gagctccgac tgcgtgacca ccggttcata gttcctcttg attattccaa
     1381 aagttctccc aaaattaccg tctttgctcg agagatcgtt gcgggtaact tgaaatttgg
     1441 agctttttga ttcagagctc tttctgtgat tacgaagcta aaagtttgtg tcttttgttt
     1501 tttgtagttg ggaaagagga gcaagcaatg ccgtatttat tgtatttgca aggtggtcca
     1561 ggatttgaag gtccaaggcc tagtgaagct agtggatgga ttcaaagagc ttgtgaagag
     1621 tttcgagtgg tcttactcga tcaagtatga tgctttcata gttttatact cttctctgga
     1681 cagttttttt agtctttttg ttgttctaat tttgtttgtc ctgcagcgag gaacaggctt
     1741 gtccactcct ttaatatgtt cctctatgct tcaattcaaa tcagcaaagg aattagctga
     1801 ttatttggtt cactttcgag ctgataatat agttaaagat gctgagttta ttagagttcg
     1861 tcttgttcct aaggctgatc cttggacaat tttgggtcag gtaatttgtc tttgtattgt
     1921 ctattggtca tcttgcttca gttcttgtca tttgattttg tattcatctt tttttcttca
     1981 aatctacaga gtttcggtgg cttttgtgca cttacatatc tgagttttgc acccgaagga
     2041 ctcaaacaag tgctaataac tggaggaatt ccaccaattg gtaaagcatg tacagcagat
     2101 gatgtatatg aagccggttt tgaacaagtt gctcgccaaa atgaaaaata ctacaagaga
     2161 ttccctcaag acattgagat tgttcgtgaa ctcgtaaatt acttggctga atctgaaggt
     2221 ggcggggtga gggattacaa catttgctca atccactgac cagtttagtt tagttcctta
     2281 cttgtctctg tttctttgtc tttaggtacc tctcccatca ggaggcattc ttactcctaa
     2341 gggtcttcaa actcttggtc tttctggttt aggatcaagt actggttttg agcgcctgca
     2401 ttatatgtaa gagtttagct taatttttat caaccacctc acatgttctg tgttaaatct
     2461 ggccttctta attgatcata ggttggagag agtatgggat cctattttag ttactggagc
     2521 tccaaagtgt attagtcagt tcttcttaaa cgctgtaaga gacccacaaa aatttgttgt
     2581 tttctgttct cagctcaaat aaggaattca atagtctgag acattttatc gttgctgcag
     2641 tttgagagtt ggcattcttt tgatgcaaat ccactatatg ctcttctcca cgaggccata
     2701 tactgtgagg tatagtctct tcaaatttct catctttttt tctatttcag acaattcctt
     2761 cttcttgtta tcatttatgt ttaaatggaa actttttcag ggtgcttcat caggatggtc
     2821 tgctcataga ttacgggata aatatgagta caaattcgac gcaatgaagg cagtcaaaga
     2881 aagtcagcct gtactcttca caggagaggt acaggttttg agtatttcta attgtgtggc
     2941 tcatagcttg gatgattggc ccacattgct taactaaaat agaatttgga tttgtataga
     3001 tgatatttcc atggatgttt gatgaaattc atgccttgaa gccatttaaa gctgccgccg
     3061 atcttttggc caagaaagag gattggcctc cattatatga tgttcctaga ttgcagaata
     3121 acaaggtctg tttcagaatc ttgatcgttc tttcatatgg atcaaataat tttactctgt
     3181 ttcacatgat ttctttttgt aggtgcctgt tgctgcagcg gtttactacg aagatatgta
     3241 tgtaaacttc aagctggtga cggaaacagc ttctcatata tcgggtatca gactttgggt
     3301 aacgaatgag tttatgcatt cgggtcttcg tgatgctgga agacaaatta ttgatcattt
     3361 gttgggaatg atcaatggga aaaagcctct cttttagggc gaattcctat ctagacccag
     3421 ctttcttgta caaagtggtg ataacctaga gctcgaattt ccccgatcgt tcaaacattt
     3481 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     3541 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     3601 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     3661 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     3721 aattggttcc ggaaccaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3841 ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct attaattccc
     3901 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     3961 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     4021 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     4081 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     4141 tgtttgaacg atcggggaaa ttcgggggat cgatcccgcg aactgtggac gagaactgtg
     4201 ccaccaagcg taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc
     4261 tgaggcgttc tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg
     4321 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     4381 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     4441 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     4501 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     4561 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     4621 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     4681 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     4741 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     4801 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     4861 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     4921 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     4981 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     5041 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaatgcccca atgtcaagca
     5101 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     5161 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     5221 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     5281 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcttattgcc
     5341 ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
     5401 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
     5461 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     5521 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     5581 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     5641 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     5701 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     5761 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     5821 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     5881 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     5941 agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat tcagcctaat
     6001 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     6061 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     6121 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     6181 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     6241 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     6301 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     6361 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     6421 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     6481 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     6541 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     6601 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     6661 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     6721 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     6781 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     6841 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     6901 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     6961 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     7021 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     7081 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     7141 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     7201 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     7261 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     7321 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     7381 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     7441 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     7501 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     7561 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8281 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8341 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8401 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8461 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8521 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     8581 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8641 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     8701 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     8761 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     8821 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     8881 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     8941 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     9001 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     9061 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     9121 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     9181 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     9241 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     9301 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     9361 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     9421 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     9481 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     9541 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     9601 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     9661 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     9721 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     9781 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     9841 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     9901 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     9961 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    10021 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    10081 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    10141 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    10201 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    10261 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
    10321 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    10381 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    10441 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    10501 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    10561 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    10621 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    10681 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    10741 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    10801 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    10861 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    10921 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    10981 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    11041 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    11101 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    11161 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    11221 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    11281 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    11341 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    11401 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    11461 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    11521 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    11581 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    11641 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    11701 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    11761 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    11821 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    11881 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    11941 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    12001 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    12061 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    12121 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    12181 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    12241 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    12301 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    12361 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    12421 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    12481 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    12541 acct
//
