LOCUS       Exported               12536 bp ds-DNA     circular SYN 03-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1219..1235
                     /label=attL1
     misc_feature    1238..1246
                     /label=pJLsmart_for
                     /note="pJLsmart is a entry vector to the Invitrogen GATEWAY
                     system. It is a derivate of pENTR1A (Invitrogen), in which 
                     the polylinker and ccdB gene have been repalced by a 
                     minimal MCS to allow for smart cloning using SmaI and SrfI.
                     Flanking EcoRI sites for easy clone identification and 
                     BamHI and HindIII sites for asymmetric cuts were also 
                     introduced."
     exon            1247..1418
                     /label=exon1
     exon            1502..1575
                     /label=exon2
     exon            1576..1638
                     /label=exon2
     exon            1721..1894
                     /label=exon3
     exon            1984..2220
                     /label=exon4
     misc_feature    1984..1986
                     /label=S
     exon            2300..2400
                     /label=exon5
     misc_feature    2476..2548
                     /label=exon6
     exon            2635..2703
                     /label=exon7
     exon            2795..2902
                     /label=exon8
     exon            2994..3119
                     /label=exon9
     exon            3197..3373
                     /label=exon10
     misc_feature    3227..3229
                     /label=D
     misc_feature    3311..3313
                     /label=H
     misc_feature    3392..3400
                     /label=pJLsmart_for
                     /note="pJLsmart is a entry vector to the Invitrogen GATEWAY
                     system. It is a derivate of pENTR1A (Invitrogen), in which 
                     the polylinker and ccdB gene have been repalced by a 
                     minimal MCS to allow for smart cloning using SmaI and SrfI.
                     Flanking EcoRI sites for easy clone identification and 
                     BamHI and HindIII sites for asymmetric cuts were also 
                     introduced."
     CDS             3406..3416
                     /codon_start=1
                     /label=Gateway uni-frame
                     /translation="DPA"
     protein_bind    complement(3407..3431)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(3407..3416)
                     /label=attL2
     misc_feature    complement(3410..3416)
                     /label=G-4001
                     /note="pJLsmart attL2_seq_primer"
     terminator      3457..3709
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3743..3759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3767..3783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3791..3821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3836..3857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3893..4145)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4296..5321)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5373..5552)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6058..6082
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6603..7394
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7640..8228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8129..8148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8382..8399
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8414..8554
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8480..8499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8640..8662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8898..9092
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9158..10231)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10660..11289)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc aggctttcga attccagaag cgggatcggt
     1261 ttacgtcgat gtagctgggg aatcgaagtc ggagcatgtt actgggaagt ggttttcggt
     1321 gccggagctc cgactgcgtg accaccggtt catagttcct cttgattatt ccaaaagttc
     1381 tcccaaaatt accgtctttg ctcgagagat cgttgcgggt aacttgaaat ttggagcttt
     1441 ttgattcaga gctctttctg tgattacgaa gctaaaagtt tgtgtctttt gttttttgta
     1501 gttgggaaag aggagcaagc aatgccgtat ttattgtatt tgcaaggtgg tccaggattt
     1561 gaaggtccaa ggcctagtga agctagtgga tggattcaaa gagcttgtga agagtttcga
     1621 gtggtcttac tcgatcaagt atgatgcttt catagtttta tactcttctc tggacagttt
     1681 ttttagtctt tttgttgttc taattttgtt tgtcctgcag cgaggaacag gcttgtccac
     1741 tcctttaaca tgttcctcta tgcttcaatt caaatcagca aaggaattag ctgattattt
     1801 ggttcacttt cgagctgata atatagttaa agatgctgag tttattagag ttcgtcttgt
     1861 tcctaaggct gatccttgga caattttggg tcaggtaatt tgtctttgta ttgtctattg
     1921 gtcatcttgc ttcagttctt gtcatttgat tttgtattca tctttttttc ttcaaatcta
     1981 cagagtttcg gtggcttttg tgcacttaca tatctgagtt ttgcacccga aggactcaaa
     2041 caagtgctaa taactggagg aattccacca attggtaaag catgtacagc agatgatgta
     2101 tatgaagccg gttttgaaca agttgctcgc caaaatgaaa aatactacaa gagattccct
     2161 caagacattg agattgttcg tgaactcgta aattacttgg ctgaatctga aggtggcggg
     2221 gtgagggatt acaacatttg ctcaatccac tgaccagttt agtttagttc cttacttgtc
     2281 tctgtttctt tgtctttagg tacctctccc atcaggaggc attcttactc ctaagggtct
     2341 tcaaactctt ggtctttctg gtttaggatc aagtactggt tttgagcgcc tgcattatat
     2401 gtaagagttt agcttaattt ttatcaacca cctcacatgt tctgtgttaa atctggcctt
     2461 cttaattgat cataggttgg agagagtatg ggatcctatt ttagttactg gagctccaaa
     2521 gtgtattagt cagttcttct taaacgctgt aagagaccca caaaaatttg ttgttttctg
     2581 ttctcagctc aaataaggaa ttcaatagtc tgagacattt tatcgttgct gcagtttgag
     2641 agttggcatt cttttgatac aaatccacta tatgctcttc tccacgaggc catatactgt
     2701 gaggtatagt ctcttcaaat ttctcatctt tttttctatt tcagacaatt ccttcttctt
     2761 gttatcattt atgtttaaat ggaaactttt tcagggtgct tcatcaggat ggtctgctca
     2821 tagattacgg gataaatatg agtacaaatt cgacgcaatg aaggcagtca aagaaagtca
     2881 gcctgtactc ttcacaggag aggtacaggt tttgagtatt tctaattgta tggctcatag
     2941 cttggatgat tggtccacat tgcttaacta aaatagaatt tggatttgta tagatgatat
     3001 ttccatggat gtttgatgaa attcatgcct tgaagccatt taaagctgcc gccgatcttt
     3061 tggccaagaa agaggattgg cctccattat atgatgttcc tagattgcag aataacaagg
     3121 tctgtttcag aatcttgatc gttctttcat atggatcaaa taattttact ctgtttcaca
     3181 tgatttcttt ttgtaggtgc ctgttgctgc agcggtttac tacgaagata tgtatgtaaa
     3241 cttcaagctg gtgacggaaa cagcttctca tatatcgggt atcagacttt gggtaacgaa
     3301 tgagtttatg cattcgggtc ttcgtgatgc tggaagacaa attattgatc atttgttggg
     3361 aatgatcaat gggtgaaagc ctctcttttg acgaattcct atctagaccc agctttcttg
     3421 tacaaagtgg tgataaccta gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     3481 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     3541 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     3601 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     3661 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     3721 ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3781 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3841 tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc ccgatctagt
     3901 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     3961 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     4021 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     4081 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     4141 cgatcgggga aattcggggg atcgatcccg cgaactgtgg acgagaactg tgccaccaag
     4201 cgtaaggccg ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt
     4261 tctcgaaatc agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg
     4321 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     4381 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     4441 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     4501 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
     4561 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     4621 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     4681 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     4741 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     4801 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     4861 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     4921 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc
     4981 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
     5041 cggcgggaga tgcaataggt caggctctcg ctgaatgccc caatgtcaag cacttccgga
     5101 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     5161 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     5221 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     5281 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcttattg ccccccggga
     5341 tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
     5401 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
     5461 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
     5521 gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
     5581 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat
     5641 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     5701 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     5761 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     5821 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     5881 tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg
     5941 cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta attcggcgtt
     6001 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     6061 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     6121 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     6181 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     6241 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     6301 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc
     6361 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     6421 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     6481 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     6541 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     6601 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     6661 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     6721 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     6781 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     6841 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     6901 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     6961 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     7021 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     7081 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     7141 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     7201 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     7261 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     7321 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     7381 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     7441 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     7501 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     7561 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8281 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8341 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8401 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8461 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     8521 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     8581 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8641 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     8701 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     8761 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     8821 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     8881 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     8941 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     9001 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     9061 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     9121 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     9181 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     9241 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     9301 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     9361 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     9421 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     9481 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     9541 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     9601 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     9661 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     9721 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     9781 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     9841 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     9901 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     9961 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
    10021 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
    10081 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
    10141 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
    10201 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
    10261 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
    10321 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
    10381 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
    10441 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
    10501 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
    10561 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
    10621 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
    10681 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
    10741 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
    10801 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
    10861 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
    10921 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
    10981 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
    11041 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
    11101 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
    11161 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
    11221 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
    11281 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
    11341 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
    11401 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
    11461 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
    11521 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
    11581 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
    11641 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
    11701 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
    11761 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
    11821 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
    11881 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
    11941 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
    12001 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
    12061 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
    12121 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
    12181 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
    12241 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
    12301 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
    12361 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
    12421 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat
    12481 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaacct
//
