LOCUS       Exported               12480 bp ds-DNA     circular SYN 03-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12480
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1219..1235
                     /label=attL1
     misc_feature    1238..1246
                     /label=pJLsmart_for
                     /note="pJLsmart is a entry vector to the Invitrogen GATEWAY
                     system. It is a derivate of pENTR1A (Invitrogen), in which 
                     the polylinker and ccdB gene have been repalced by a 
                     minimal MCS to allow for smart cloning using SmaI and SrfI.
                     Flanking EcoRI sites for easy clone identification and 
                     BamHI and HindIII sites for asymmetric cuts were also 
                     introduced."
     exon            1269..1376
                     /label=exon1
     exon            1460..1596
                     /label=exon2
     exon            1679..1852
                     /label=exon3
     exon            1942..2178
                     /label=exon4
     exon            2258..2358
                     /label=exon5
     exon            2434..2506
                     /label=exon6
     exon            2593..2661
                     /label=exon7
     exon            2753..2860
                     /label=exon8
     exon            2952..3077
                     /label=exon9
     exon            3155..3331
                     /label=exon10
     misc_feature    3335..3344
                     /label=pJLsmart_for
                     /note="pJLsmart is a entry vector to the Invitrogen GATEWAY
                     system. It is a derivate of pENTR1A (Invitrogen), in which 
                     the polylinker and ccdB gene have been repalced by a 
                     minimal MCS to allow for smart cloning using SmaI and SrfI.
                     Flanking EcoRI sites for easy clone identification and 
                     BamHI and HindIII sites for asymmetric cuts were also 
                     introduced."
     CDS             3350..3360
                     /codon_start=1
                     /label=Gateway uni-frame
                     /translation="DPA"
     protein_bind    complement(3351..3375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(3351..3360)
                     /label=attL2
     misc_feature    complement(3354..3360)
                     /label=G-4001
                     /note="pJLsmart attL2_seq_primer"
     terminator      3401..3653
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3687..3703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3711..3727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3735..3765)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3780..3801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3837..4089)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4240..5265)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5317..5496)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6002..6026
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6547..7338
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7584..8172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8358..8498
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8842..9036
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9102..10175)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10604..11233)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc aggctttcga attccaagga gcatgttact
     1261 gggaagttgg ttttcggtgc cggagctccg actgcgtgac caccggttca tagttcctct
     1321 tgattattcc aaaagttctc ccaaaattac cgtctttgct cgagagatcg ttgcgggtaa
     1381 cttgaaattt ggagcttttt gattcagagc tctttctgtg attacgaagc taaaagtttg
     1441 tgtcttttgt tttttgtagt tgggaaagag gagcaagcaa tgccgtattt attgtatttg
     1501 caaggtggtc caggatttga aggtccaagg cctagtgaag ctagtggatg gattcaaaga
     1561 gcttgtgaag agtttcgagt ggtcttactc gatcaagtat gatgctttca tagttttata
     1621 ctcttctctg gacagttttt ttagtctttg tgttgttcta attttgtttg tcctgcagcg
     1681 aggaacaggc ttgtccactc ctttaacatg ttcctctatg cttcaattca aatcagcaaa
     1741 ggaattagct gattatttgg ttcactttcg agctgataat atagttaaag atgctgagtt
     1801 tattagagtt cgtcttgttc ctaaggctga tccttggaca attttgggtc aggtaatttg
     1861 tctttgtatt gtctattggt catcttgctt cagttcttgt catttgattt tgtattcatc
     1921 tttttttctt caaatctaca gagtttcggt ggcttttgtg cacttacata tctgagtttt
     1981 gcacccgaag gactcaaaca agtgctaata actggaggaa ttccaccaat tggtaaagca
     2041 tgtacagcag atgatgtata tgaagccggt tttgaacaag ttgctcgcca aaatgaaaaa
     2101 tactacaaga gattccctca agacattgag attgttcgtg aactcgtaaa ttacttggct
     2161 gaatctgaag gtggcggggt gagggattac aacatttgct caatccactg accagtttag
     2221 tttagttcct tacttgtctc tgtttctttg tctttaggta cctctcccat caggaggcat
     2281 tcttactcct aagggtcttc aaactcttgg tctttctggt ttaggatcaa gtactggttt
     2341 tgagcgcctg cattatatgt aagagtttag cttaattttt atcaaccacc tcacatgttc
     2401 tgtgttaaat ctggccttct taattgatca taggttggag agagtatggg atcctatttt
     2461 agttactgga gctccaaagt gtattagtca gttcttctta aacgctgtaa gagacccaca
     2521 aaaatttgtt gttttctgtt ctcagctcaa ataaggaatt caatagtctg agacatttta
     2581 tcgttgctgc agtttgagag ttggcattct tttgatacaa atccactata tgctcttctc
     2641 cacgaggcca tatactgtga ggtatagtct cttcaaattt ctcatctttt tttctatttc
     2701 agacaattac ttcttcttgt tatcgtttat gtttaaatgg aaactttttc agggtgcttc
     2761 atcaggatgg tctgctcata gattacggga taaatatgag tacaaattcg acgcaatgaa
     2821 ggcagtcaaa gaaagtcagc ctgtactctt cacaggagag gtacaggttt tgagtatttc
     2881 taattgtatg gctcatagct tggatgattg gtccacattg cttaactaaa atagaatttg
     2941 gatttgtata gatgatattt ccatggatgt ttgatgaaat tcatgccttg aagccattta
     3001 aagctgccgc cgatcttttg gccaagaaag aggattggcc tccattatat gatgttccta
     3061 gattgcagaa taacaaggtc tgtttcagaa tcttgatcgt tctttcatat ggatcaaata
     3121 attttactct gtttcacatg atttcttttt gtaggtgcct gttgctgcag cggtttacta
     3181 cgaagatatg tatgtaaact tcaagctggt gacggaaaca gcttctcata tatcgggtat
     3241 cagactttgg gtaacgaatg agtttatgca ttcgggtctt cgtgatgctg gaagacaaat
     3301 tattgatcat ttgttgggaa tgatcaatgg gtgaccgaat tcctatctag acccagcttt
     3361 cttgtacaaa gtggtgataa cctagagctc gaatttcccc gatcgttcaa acatttggca
     3421 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3481 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3541 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3601 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3661 ggttccggaa ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3781 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc
     3841 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     3901 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     3961 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     4021 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     4081 tgaacgatcg gggaaattcg ggggatcgat cccgcgaact gtggacgaga actgtgccac
     4141 caagcgtaag gccgttctct cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag
     4201 gcgttctcga aatcagctct tgttcggtcg gcatctactc tattcctttg ccctcggacg
     4261 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     4321 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     4381 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     4441 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     4501 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     4561 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     4621 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     4681 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     4741 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     4801 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     4861 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     4921 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     4981 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat gccccaatgt caagcacttc
     5041 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     5101 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     5161 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     5221 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctt attgcccccc
     5281 gggatcagat ctggattgag agtgaatatg agactctaat tggataccga ggggaattta
     5341 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     5401 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     5461 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     5521 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct
     5581 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     5641 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     5701 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     5761 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     5821 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     5881 cttgcggcag cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg
     5941 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     6001 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     6061 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     6121 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     6181 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     6241 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     6301 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     6361 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     6421 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     6481 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     6541 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     6601 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     6661 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     6721 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     6781 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     6841 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     6901 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     6961 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     7021 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     7081 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     7141 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     7201 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     7261 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     7321 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     7381 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     7441 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     7501 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7561 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7621 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7681 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7741 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7801 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7861 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7921 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7981 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8041 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8101 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8161 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8221 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8281 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8341 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8401 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8461 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     8521 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8581 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     8641 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     8701 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     8761 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     8821 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     8881 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     8941 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     9001 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     9061 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     9121 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     9181 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     9241 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     9301 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     9361 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     9421 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     9481 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     9541 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     9601 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     9661 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     9721 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     9781 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     9841 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     9901 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     9961 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    10021 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    10081 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    10141 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    10201 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
    10261 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    10321 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    10381 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    10441 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    10501 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    10561 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    10621 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    10681 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    10741 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    10801 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    10861 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    10921 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    10981 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    11041 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    11101 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    11161 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    11221 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    11281 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    11341 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    11401 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    11461 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    11521 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    11581 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    11641 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    11701 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    11761 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    11821 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    11881 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    11941 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    12001 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    12061 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    12121 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    12181 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    12241 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    12301 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    12361 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    12421 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
