LOCUS       pFREDusk-KanR-MCS       7875 bp ds-DNA     circular     10-DEC-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(26..73)
                     /label="T7 terminator_regulatory"
     misc_feature    complement(140..157)
                     /label="6xHis"
     misc_feature    179..237
                     /label="MCS"
     CDS             complement(205..237)
                     /label="T7 gene (10 leader)"
                     /translation="MASMTGGQQMG"
     misc_feature    complement(241..258)
                     /label="Thrombin site"
     CDS             complement(241..258)
                     /label="Thrombin site"
                     /translation="LVPRGS"
     misc_feature    complement(268..285)
                     /label="6xHis"
     CDS             complement(268..285)
                     /label="6xHis"
                     /translation="HHHHHH"
     RBS             304..326
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(339..585)
                     /label="FixK2 promoter_regulatory"
     primer_bind     complement(655..674)
                     /label="pBRrevBam"
     promoter        880..957
                     /label="lacIq promoter"
     misc_feature    958..960
                     /label="BsYtvA-LOV"
     misc_feature    961..1563
                     /label="DrHo1"
     misc_feature    1563..3074
                     /label="PaBphP (1-504)"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
     CDS             1563..3755
                     /translation="MTSITPVTLANCEDEPIHVPGAIQPHGALVTLRADGMVLAASENIQALLGFVASPGSYLTQEQVGPEVLRMLEEGLTGNGPWSNSVETRIGEHLFDVIGHSYKEVFYLEFEIRTADTLSITSFTLNAQRIIAQVQLHNDTASLLSNVTDELRRMTGYDRVMAYRFRHDDSGEVVAESRREDLESYLGQRYPASDIPAQARRLYIQNPIRLIADVAYTPMRVFPALNPETNESFDLSYSVLRSVSPIHCEYLTNMGVRASMSISIVVGGKLWGLFSCHHMSPKLIPYPVRMSFQIFSQVCSAIVERLEQGRIAELLRVSTERRLALARRARDADDLFGALAHPDDGIAALIPCDGALVMLGGRTLSIRGDFERQAGNVLQRLQRDPERDIYHTDNWPQPSEDSPDGGDCCGVLAIRFHRQESGWIFWFRHEEVHRIRWGGKPEKLLTIGPSGPRLTPRGSFEAWEEVVRGHSTPWSETDLAIAEKLRLDLMELCLNHAAEVDRMRMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVARGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNLFRNALEAMAQSQRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLSISRSIIEAHGGRMWAESNASGGATFRFTLPAADEMIGGLA*"
     misc_feature    3054..3074
                     /label="Linker"
     misc_feature    3075..3752
                     /label="BjFixL-HK"
     misc_feature    3752..4366
                     /label="FixJ"
     CDS             5170..5361
                     /label="rop"
     misc_feature    5463..5605
                     /label="bom"
     rep_origin      complement(5773..6455)
                     /label="ColE1 origin_rep_origin"
     CDS             6501..7316
                     /label="KanR resistance marker_CDS"
     rep_origin      complement(7409..7864)
                     /label="M13 origin_rep_origin"
     rep_origin      complement(7541..7847)
                     /label="F1 origin_rep_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgcTC GAGTGCGGCC GCAAGCTTGT
      181 CGACGGAGCT CGAATTCGGA TCCGACCCAT TTGCTGTCCA CCAGTCATGC TAGCCATATG
      241 GCTGCCGCGC GGCACCAGGC CGCTGCTGTG ATGATGATGA TGATGGCTGC TGCCCATGGT
      301 ATATCTCCTT CTTAAAGTTA AACAAAATTA TTTctagact ccgttgtgat gacgcattgg
      361 tacgcggtat cgggaggttc gaaaatttcg agcgatatct taaggggggt gccttacgta
      421 gaaccccgta ggtcatgccc gaggccggtc ctggatggcg cggcggatac gcttgagcag
      481 gttttcgtcg agaagcggct tcaaaaccac gtcttttacg ccggcctcgg cggcccgggt
      541 cgagatgttt tcgtccggat agccggtgat caggatcacg ggcgtagatc tcgatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      721 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      781 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
      841 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggtgcaaa
      901 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattgagggt ggtgaatgtg
      961 CTCCGACTCA TGATCATGAA GCTTTTACAA AGGGAGACCC TGGACCTGCA CCGCGAGGTC
     1021 GAGGCCCTGA TGCCCGTGAT GAGTGAGACG CTGACCCGGC GGGGCTATCT GGAGGTGCTG
     1081 CGGCGCCTGC AAGCGGTGGT CGCGCCGCTG GAAGCACAAC TCGACGCCCT GCCGCTGCCG
     1141 GACGAGCTGG CGTGGCCCGA GCGCCGCAAG GCCCATCTGC TCGCTCTGGA CCTTGCCGTA
     1201 ATGGGGGAAG GGCCGTCAAA TCAGCAGGCG CCACTGTCTG TGCCCGAGCT GACCACTCTG
     1261 GCGCAGGCCT TCGGAGCGCT TTACGTGCTT GAAGGCGCGA CGCTAGGCGG ACAGCTCATC
     1321 AGCCGCCATC TCAGGCGCAC GCTGGGGCTA AGCCCTGAGC AGGGCAGCGC CTATTTCTCC
     1381 GGCTATGGCC CCCAGACCGG CCCGCGCTGG CGCTCGTTCG GGGAAGTGCT GGAAGCCAGC
     1441 GTGCCGGCAG AAGACGCGGC AGAGGTCGTG GCGGGAGCGC GGCAGACCTT CGGGGCCTTC
     1501 CGCCGCGCCT TGCAGGGGCT GTCGGAAGCT GAAGCGGTCC AAGTGCCTGA GGTCGCCCAC
     1561 GCATGACGAG CATCACCCCG GTTACCCTGG CGAACTGCGA GGACGAACCC ATCCACGTGC
     1621 CTGGCGCGAT CCAGCCGCAC GGGGCGCTGG TCACCCTGCG CGCGGACGGC ATGGTCCTCG
     1681 CGGCAAGCGA GAACATCCAG GCGCTACTGG GGTTCGTCGC CTCGCCGGGT TCCTACCTGA
     1741 CGCAGGAGCA GGTGGGGCCG GAGGTCCTGC GCATGCTCGA AGAGGGACTG ACCGGCAACG
     1801 GCCCATGGTC GAACAGCGTC GAGACGCGGA TCGGCGAACA CCTGTTCGAC GTGATCGGCC
     1861 ACAGCTACAA GGAAGTGTTC TACCTGGAGT TCGAGATCCG CACCGCGGAC ACGCTGTCGA
     1921 TCACCTCCTT CACCCTCAAC GCCCAGCGCA TCATCGCCCA GGTCCAGTTG CACAACGACA
     1981 CCGCCAGCCT GCTCAGCAAC GTCACCGACG AACTGCGCCG GATGACCGGC TACGACCGGG
     2041 TGATGGCCTA CCGTTTCCGC CATGACGACT CCGGCGAGGT GGTCGCCGAG AGCCGTCGCG
     2101 AAGACCTCGA AAGCTACCTG GGGCAACGCT ACCCGGCCTC GGACATCCCG GCCCAGGCGC
     2161 GTCGCCTGTA CATCCAGAAC CCGATCAGGC TGATCGCCGA CGTGGCCTAT ACGCCGATGC
     2221 GCGTGTTTCC CGCGCTGAAC CCGGAGACCA ACGAATCGTT CGATCTCAGC TACAGCGTGC
     2281 TGCGCAGCGT TTCGCCGATC CACTGCGAAT ACCTGACCAA CATGGGCGTG CGCGCCTCGA
     2341 TGAGCATCTC CATCGTGGTC GGCGGCAAGC TCTGGGGACT GTTCTCCTGC CATCACATGT
     2401 CGCCCAAACT GATTCCCTAC CCGGTGCGCA TGTCGTTCCA GATCTTCTCC CAGGTCTGCA
     2461 GCGCCATCGT CGAGCGCCTG GAGCAGGGGC GTATCGCCGA ACTGCTGCGC GTGTCCACCG
     2521 AGCGCAGGCT GGCCCTGGCT CGCCGCGCGC GGGACGCCGA CGACCTGTTC GGCGCACTGG
     2581 CCCACCCGGA CGATGGCATC GCCGCACTGA TTCCCTGCGA CGGCGCCCTG GTCATGCTCG
     2641 GCGGCCGCAC CCTGAGCATC CGCGGCGACT TCGAGCGCCA GGCCGGGAAT GTCCTGCAAC
     2701 GCCTGCAGCG CGACCCGGAA CGGGACATCT ACCACACCGA CAACTGGCCG CAGCCCAGCG
     2761 AGGACTCTCC GGACGGCGGC GACTGCTGCG GTGTGCTGGC GATCCGTTTC CACCGCCAGG
     2821 AATCGGGCTG GATCTTCTGG TTCCGCCACG AGGAAGTGCA CCGCATCCGC TGGGGCGGCA
     2881 AGCCGGAAAA GCTGTTGACC ATCGGCCCCT CCGGCCCGCG GCTGACCCCG CGCGGCTCGT
     2941 TCGAGGCCTG GGAGGAAGTG GTCCGCGGCC ACTCCACGCC CTGGAGCGAA ACCGACCTGG
     3001 CGATCGCCGA AAAGCTGCGC CTGGACCTGA TGGAACTGTG CCTCAACCAT GCGGCGGAAG
     3061 TTGATCGTAT GCGTatggcg tccgcgctcg cgcacgagct caaccagccg ctggcggcga
     3121 tcagcaacta catgaagggc tcgcggcggc tgcttgccgg cagcagtgat ccgaacacac
     3181 cgaaggtcga aagcgccctg gaccgcgccg ccgagcaggc gctgcgcgcc ggccagatca
     3241 tccggcgcct gcgcgacttc gttgcccgcg gcgaatcgga gaagcgggtc gagagtctct
     3301 ccaagctgat cgaggaggcc ggcgcgctcg ggcttgccgg cgcgcgcgag cagaacgtgc
     3361 agctccgctt cagtctcgat ccgggcgccg atctcgttct cgccgaccgg gtgcagatcc
     3421 agcaggtcct ggtcaacctg ttccgcaacg cgctggaagc gatggctcag tcgcagcgac
     3481 gcgagctcgt cgtcaccaac acccccgccg ccgacgacat gatcgaggtc gaagtgtccg
     3541 acaccggcag cggtttccag gacgacgtca ttccgaacct gtttcagact ttcttcacca
     3601 ccaaggacac cggcatgggc gtgggactgt ccatcagccg ctcgatcatc gaagctcacg
     3661 gcgggcgcat gtgggccgag agcaacgcat cgggcggggc gaccttccgc ttcaccctcc
     3721 cggcagccga cgagatgata ggaggtctag catgacgacc aagggacata tctacgtcat
     3781 cgacgacgac gcggcgatgc gggattcgct gaatttcctg ctggattctg ccggcttcgg
     3841 cgtcacgctg tttgacgacg cgcaagcctt tctcgacgcc ctgccgggtc tctccttcgg
     3901 ctgcgtcgtc tccgacgtgc gcatgccggg ccttgacggc atcgagctgt tgaagcggat
     3961 gaaggcgcag caaagcccct ttccgatcct catcatgacc ggtcacggcg acgtgccgct
     4021 cgcggtcgag gcgatgaagt taggggcggt ggactttctg gaaaagcctt tcgaggacga
     4081 ccgcctcacc gccatgatcg aatcggcgat ccgccaggcc gagccggccg ccaagagcga
     4141 ggccgtcgcg caggatatcg ccgcccgcgt cgcctcgttg agccccaggg agcgccaggt
     4201 catggaaggg ctgatcgccg gcctttccaa caagctgatc gcccgcgagt acgacatcag
     4261 cccgcgcacc atcgaggtgt atcgggccaa cgtcatgacc aagatgcagg ccaacagcct
     4321 ttcggagctg gttcgcctcg cgatgcgcgc cggcatgctc aacgattgac aattgatgta
     4381 agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca
     4441 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     4501 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     4561 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     4621 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     4681 attatcgccg gcatggcggc cccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     4741 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     4801 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     4861 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     4921 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     4981 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     5041 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     5101 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     5161 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     5221 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     5281 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     5341 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5401 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5461 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     5521 catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg
     5581 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5641 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5701 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6421 gaacgaaaac tcacgttaag ggattttggt catgaacaat aaaactgtct gcttacataa
     6481 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc
     6541 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     6601 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     6661 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     6721 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     6781 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     6841 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     6901 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     6961 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     7021 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     7081 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     7141 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     7201 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     7261 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat
     7321 taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7381 cgcacatttc cccgaaaagt gccacctGAA attgtaaacg ttaatatttt gttaaaattc
     7441 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     7501 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     7561 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     7621 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     7681 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     7741 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     7801 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     7861 gcgtcccatt cgcca
//