LOCUS       pTYB1-MBD2∆C∆GR_pc5079  8101 bp ds-DNA     circular     07-MAY-2025
DEFINITION  .
KEYWORDS    "marker:AmpR, MBD2∆C∆GR"
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_fwdcolor="#ffff00"
                     /note="label: ori note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=rep_origin"
     misc_feature    complement(766..856)
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: bom (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=64.5 note: /other=misc_feature"
     CDS             complement(958..1149)
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: rop note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=99.5 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL*"
     CDS             1190..1300
                     /label="mbeC (fragment)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: mbeC (fragment) note: pLannotate note: /database=swissprot note: /fragment=True note: /identity=100.0 note: /match_length=32.2 note: /other=CDS transl_table: 1 translation: MIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRR"
     CDS             complement(1335..1657)
                     /label="TcR (fragment)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: TcR (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=27.1 note: /other=CDS transl_table: 1 translation: STDALESLQPSQLLPVGAGHDYRRRTYDCLLYHATRRTGAGSALGHFRRGPLSLERDDDRPVACGIRNLARPRSSLRHWSRHQTFRREAGHYRRHGGRRAGLRLAGV"
     protein_bind    complement(1672..1693)
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: CAP binding site note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=95.5 note: /match_length=100.0 note: /other=protein_bind"
     CDS             complement(1706..2788)
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: lacI note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=99.9 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     promoter        complement(2789..2866)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: lacI promoter note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=promoter"
     CDS             complement(2874..2967)
                     /label="TcR (fragment)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: TcR (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=7.9 note: /other=CDS transl_table: 1 translation: ACTIPCGGGAQRPQPTTGLLPNAGVA*GRAS"
     terminator      3019..3105
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: rrnB T1 terminator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=terminator"
     terminator      3202..3288
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: rrnB T1 terminator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=terminator"
     terminator      3385..3471
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: rrnB T1 terminator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=terminator"
     terminator      3568..3654
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: rrnB T1 terminator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=terminator"
     terminator      3751..3837
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: rrnB T1 terminator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=terminator"
     promoter        3972..3990
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: T7 promoter note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=promoter"
     protein_bind    3991..4015
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: lac operator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=protein_bind"
     RBS             4030..4052
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: RBS note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=RBS"
     CDS             4084..4722
                     /label="MBD2∆C∆GR"
                     /ApEinfo_revcolor="#ff6600"
                     /ApEinfo_fwdcolor="#ff6600"
                     /note="codon_start: 1 label: MBD2 (fragment) note: pLannotate note: /database=swissprot note: /fragment=True note: /identity=84.3 note: /match_length=51.8 note: /other=CDS transl_table: 1 translation: MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAARGGGVCPQSGGSGLGGDGGGGAGGCGVGSGGGVAPRRDPVPFPSGSSGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNAVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQ"
     CDS             4735..5553
                     /label="Sce VMA intein 5' region"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: Sce VMA intein 5' region note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             5556..6086
                     /label="Sce VMA intein 3' region"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: Sce VMA intein 3' region note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=99.8 note: /match_length=96.7 note: /other=CDS transl_table: 1 translation: WYSQ*S*Y*ESIMGRYCWLRILEGRCQKYSFFLVYGQYRYS*NISCWSN*F*WLCY**AWY*SNNKDNSYFCQRWFGFPCSFFRLSSLG*RRTC*G*HECHQT*N*LCYLYVWWRCFA*RSFEVCRL*KIQACSRRCFCT*VPRILFRVTRIEGRRLLWDYFI**F*SSVFAWIPGC"
     CDS             6130..6285
                     /label="CBD"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: CBD note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ"
     CDS             6307..6356
                     /label="SET1 (fragment)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: SET1 (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=39.7 note: /other=CDS transl_table: 1 translation: AANKARKEAELAAATA"
     terminator      6364..6411
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: T7 terminator note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=terminator"
     promoter        6471..6575
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR promoter note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=promoter"
     CDS             6576..7436
                     /label="AmpR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: AmpR note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=99.8 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      complement(7478..7991)
                     /label="M13 ori"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_fwdcolor="#ffff00"
                     /note="direction: LEFT label: M13 ori note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=rep_origin"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagctatgg tgcactctca
      781 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
      841 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      901 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      961 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     1021 gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     1081 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     1141 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     1201 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     1261 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     1321 gggtcaatgc cagccgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc
     1381 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     1441 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     1501 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     1561 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     1621 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
     1681 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     1801 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
     1861 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
     1921 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
     1981 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
     2041 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
     2101 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
     2161 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
     2221 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
     2281 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
     2341 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
     2401 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
     2461 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
     2521 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
     2581 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
     2641 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
     2701 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
     2761 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
     2821 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
     2881 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     2941 accgccgccg caaggaatgg tgcatgccgg catgccgccc tttcgtcttc aagaattaat
     3001 tcccaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3061 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     3121 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     3181 aggaattaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3241 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     3301 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3361 gccaggaatt aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3421 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     3481 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3541 actgccagga attaattccc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     3601 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     3661 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     3721 taaactgcca ggaattaatt ccccaggcat caaataaaac gaaaggctca gtcgaaagac
     3781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     3841 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     3901 ccataaactg ccaggaattg gggatcggaa ttaattcccg gtttaaaccg gggatctcga
     3961 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     4021 agaaataatt ttgtttaact ttaagaagga gatatacata tgtcaaaggc ctacgtcgac
     4081 gccatgcgcg cgcacccggg gggaggccgc tgctgcccgg agcaggagga gggggagagc
     4141 gcggcgggcg gcagcggcgc tggcggcgac tccgccatag agcagggggg ccagggcagc
     4201 gcgctcgctc cgtccccggt gagcggcgtg cgcagggaag gcgctcgggg cggcggccgt
     4261 ggccgggggc ggtggaagca ggcggcccgg ggcggcggcg tctgtcccca gagtggcggc
     4321 agcggccttg gcggcgacgg cggcggcggc gcgggcggct gcggcgtcgg cagcggtggc
     4381 ggcgtcgccc cccggcggga tcctgtccct ttcccgtcgg ggagctcggg gccggggccc
     4441 aggggacccc gggccacgga gagcgggaag aggatggact gcccggccct cccccccgga
     4501 tggaagaagg aggaagtgat ccgaaaatca gggctcagtg ctggcaagag cgatgtctac
     4561 tacttcagtc caagtggtaa gaagttcaga agtaaacctc agctggcaag atacctggga
     4621 aatgctgttg accttagcag ttttgacttc aggaccggca agatgatgcc tagtaaatta
     4681 cagaagaaca agcagagact ccggaatgac cccctcaatc aggagggctc ttcctgcttt
     4741 gccaagggta ccaatgtttt aatggcggat gggtctattg aatgtattga aaacattgag
     4801 gttggtaata aggtcatggg taaagatggc agacctcgtg aggtaattaa attgcccaga
     4861 ggaagagaaa ctatgtacag cgtcgtgcag aaaagtcagc acagagccca caaaagtgac
     4921 tcaagtcgtg aagtgccaga attactcaag tttacgtgta atgcgaccca tgagttggtt
     4981 gttagaacac ctcgtagtgt ccgccgtttg tctcgtacca ttaagggtgt cgaatatttt
     5041 gaagttatta cttttgagat gggccaaaag aaagcccccg acggtagaat tgttgagctt
     5101 gtcaaggaag tttcaaagag ctacccaata tctgaggggc ctgagagagc caacgaatta
     5161 gtagaatcct atagaaaggc ttcaaataaa gcttattttg agtggactat tgaggccaga
     5221 gatctttctc tgttgggttc ccatgttcgt aaagctacct accagactta cgctccaatt
     5281 ctttatgaga atgaccactt tttcgactac atgcaaaaaa gtaagtttca tctcaccatt
     5341 gaaggtccaa aagtacttgc ttatttactt ggtttatgga ttggtgatgg attgtctgac
     5401 agggcaactt tttcggttga ttccagagat acttctttga tggaacgtgt tactgaatat
     5461 gctgaaaagt tgaatttgtg cgccgagtat aaggacagaa aagaaccaca agttgccaaa
     5521 actgttaatt tgtactctaa agttgtcaga ggtaatggta ttcgcaataa tcttaatact
     5581 gagaatccat tatgggacgc tattgttggc ttaggattct tgaaggacgg tgtcaaaaat
     5641 attccttctt tcttgtctac ggacaatatc ggtactcgtg aaacatttct tgctggtcta
     5701 attgattctg atggctatgt tactgatgag catggtatta aagcaacaat aaagacaatt
     5761 catacttctg tcagagatgg tttggtttcc cttgctcgtt ctttaggctt agtagtctcg
     5821 gttaacgcag aacctgctaa ggttgacatg aatgtcacca aacataaaat tagttatgct
     5881 atttatatgt ctggtggaga tgttttgctt aacgttcttt cgaagtgtgc cggctctaaa
     5941 aaattcaggc ctgctcccgc cgctgctttt gcacgtgagt gccgcggatt ttatttcgag
     6001 ttacaagaat tgaaggaaga cgattattat gggattactt tatctgatga ttctgatcat
     6061 cagtttttgc ttggatccca ggttgtcgtc catgcatgcg gtggcctgac cggtctgaac
     6121 tcaggcctca cgacaaatcc tggtgtatcc gcttggcagg tcaacacagc ttatactgcg
     6181 ggacaattgg tcacatataa cggcaagacg tataaatgtt tgcagcccca cacctccttg
     6241 gcaggatggg aaccatccaa cgttcctgcc ttgtggcagc ttcaatgact gcaggaaggg
     6301 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     6361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     6421 ggaactatat ccggataact acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgtttccca atgatgagca
     6781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     6841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7441 cagaccaagt ttactcatat atactttaga ttgatttacc ccggttgata atcagaaaag
     7501 ccccaaaaac aggaagattg tataagcaaa tatttaaatt gtaaacgtta atattttgtt
     7561 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7621 caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg
     7681 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7741 tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg
     7801 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7861 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7921 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7981 acagggcgcg taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8101 c
//