LOCUS       pEGFP-MBD2-N∆GR_pc5087  5110 bp ds-DNA     circular     07-MAY-2025
DEFINITION  .
KEYWORDS    "marker:NeoR/KanR, MBD2-N∆GR"
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_fwdcolor="#ffff00"
                     /note="label: ori note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=99.8 note: /match_length=100.0 note: /other=rep_origin"
     enhancer        764..1067
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: CMV enhancer note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=enhancer"
     promoter        1068..1271
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: CMV promoter note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=promoter"
     misc_feature    1294..1337
                     /label="MCS (fragment)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: MCS (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=54.3 note: /other=misc_feature"
     CDS             1357..1746
                     /label="MBD2-N∆GR"
                     /ApEinfo_revcolor="#ff6600"
                     /ApEinfo_fwdcolor="#ff6600"
                     /note="codon_start: 1 label: MBD2 (fragment) note: pLannotate note: /database=swissprot note: /fragment=True note: /identity=76.3 note: /match_length=31.6 note: /other=CDS transl_table: 1 translation: MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAARGGGVCPQSGGSGLGGDGGGGAGGCGVGSGGGVAPRRDPVPFPSGSSGPGPRGPRATESGKR"
     CDS             1759..2478
                     /label="EGFP"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: EGFP note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     CDS             2495..2578
                     /label="large T antigen (fragment)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: large T antigen (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=3.9 note: /other=CDS transl_table: 1 translation: DHNQPYHICRGFTCFKKPPTPPPEPET*"
     polyA_signal    2601..2722
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: SV40 poly(A) signal note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=polyA_signal"
     rep_origin      complement(2756..3184)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_fwdcolor="#ffff00"
                     /note="direction: LEFT label: f1 ori note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=rep_origin"
     promoter        3211..3315
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR promoter note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=promoter"
     promoter        3317..3674
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: SV40 promoter note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=promoter"
     CDS             3709..4503
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 label: NeoR/KanR note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=100.0 note: /match_length=100.0 note: /other=CDS transl_table: 1 translation: MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             4524..4676
                     /label="ble (fragment)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: ble (fragment) note: pLannotate note: /database=swissprot note: /fragment=True note: /identity=100.0 note: /match_length=40.5 note: /other=CDS transl_table: 1 translation: MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHP"
     polyA_signal    4735..4782
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: HSV TK poly(A) signal note: pLannotate note: /database=snapgene note: /fragment=False note: /identity=98.0 note: /match_length=100.0 note: /other=polyA_signal"
     polyA_signal    complement(4873..4889)
                     /label="bGH poly(A) signal (fragment)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: bGH poly(A) signal (fragment) note: pLannotate note: /database=snapgene note: /fragment=True note: /identity=100.0 note: /match_length=8.2 note: /other=polyA_signal"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
     1321 gctcaagctt cgaattcaaa ggcctacgtc gacgccatgc gcgcgcaccc ggggggaggc
     1381 cgctgctgcc cggagcagga ggagggggag agcgcggcgg gcggcagcgg cgctggcggc
     1441 gactccgcca tagagcaggg gggccagggc agcgcgctcg ctccgtcccc ggtgagcggc
     1501 gtgcgcaggg aaggcgctcg gggcggcggc cgtggccggg ggcggtggaa gcaggcggcc
     1561 cggggcggcg gcgtctgtcc ccagagtggc ggcagcggcc ttggcggcga cggcggcggc
     1621 ggcgcgggcg gctgcggcgt cggcagcggt ggcggcgtcg ccccccggcg ggatcctgtc
     1681 cctttcccgt cggggagctc ggggccgggg cccaggggac cccgggccac ggagagcggg
     1741 aagagggcgg ccgcagccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1801 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1861 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1921 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1981 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2041 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2101 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2161 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2221 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2281 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2341 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2401 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2461 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
     2521 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2581 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2701 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     2761 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2821 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2881 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2941 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3001 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3061 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3121 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3181 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3301 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3361 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3421 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3481 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3601 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3661 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3721 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3781 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3841 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3901 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     3961 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4021 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4081 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4141 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4201 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4261 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4321 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4381 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4441 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4501 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4561 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4621 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4681 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4741 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     4801 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4861 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     4921 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     4981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5101 aaggatcttc
//