LOCUS       pL336__pU6_osp_g        3192 bp ds-DNA     linear       21-AUG-2023
DEFINITION  ATCC 37254: very usable general purpose vector.
KEYWORDS    ATCC.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        complement(3027..3027)
                     /vntifkey="30"
                     /note="bla gene promoter"
                     /locus_tag="P(BLA)"
                     /label="P(BLA)"
                     /ApEinfo_label="P(BLA)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2135..2992)
                     /vntifkey="4"
                     /note="bla gene- Ap(r) determinant"
                     /locus_tag="AP(R)"
                     /label="AP(R)"
                     /ApEinfo_label="AP(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(1013..1013)
                     /vntifkey="30"
                     /note="lac promoter"
                     /locus_tag="P(LAC)"
                     /label="P(LAC)"
                     /ApEinfo_label="P(LAC)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1373..1373
                     /vntifkey="33"
                     /note="RNaseH cleavage point"
                     /locus_tag="ORI"
                     /label="ORI"
                     /ApEinfo_label="ORI"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1000..1019)
                     /locus_tag="0721"
                     /label="0721"
                     /ApEinfo_label="0721"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        402..642
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /locus_tag="U6 promoter"
                     /label="U6 promoter"
                     /ApEinfo_label="U6 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     402..422
                     /note="Human U6 promoter, forward primer"
                     /locus_tag="hU6-F"
                     /label="hU6-F"
                     /ApEinfo_label="hU6-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     573..592
                     /note="Human U6 promoter, forward primer"
                     /locus_tag="LKO.1 5'"
                     /label="LKO.1 5'"
                     /ApEinfo_label="LKO.1 5'"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    652..657
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    684..689
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    691..694
                     /note="U6 terminator"
                     /locus_tag="sp.gRNA"
                     /label="sp.gRNA"
                     /ApEinfo_label="sp.gRNA"
                     /ApEinfo_fwdcolor="#fa10ff"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    761..786
                     /note="U6 terminator"
                     /locus_tag="sp.gRNA(1)"
                     /label="sp.gRNA(1)"
                     /ApEinfo_label="sp.gRNA"
                     /ApEinfo_fwdcolor="#fa10ff"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    695..702
                     /locus_tag="optimized"
                     /label="optimized"
                     /ApEinfo_label="optimized"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    737..760
                     /locus_tag="optimized(1)"
                     /label="optimized(1)"
                     /ApEinfo_label="optimized"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        708..726
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
                     /locus_tag="MS2 stem loop"
                     /label="MS2 stem loop"
                     /ApEinfo_label="MS2 stem loop"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    821..850
                     /note="U6 terminator"
                     /locus_tag="sp.gRNA(2)"
                     /label="sp.gRNA(2)"
                     /ApEinfo_label="sp.gRNA"
                     /ApEinfo_fwdcolor="#fa10ff"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        792..810
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
                     /locus_tag="MS2 stem loop(1)"
                     /label="MS2 stem loop(1)"
                     /ApEinfo_label="MS2 stem loop"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagaccgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagggccta tttcccatga
      421 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
      481 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      541 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      601 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacgGAC
      661 ATACAAGTCG ACAATAGgga taccgtctct gtttCAGAGC TAggccaaca tgaggatcac
      721 ccatgtctgc agggccTGCT GGAAACAGCA TAGCAAGTTG aaataaggct agtccgttat
      781 caacttggcc aacatgagga tcacccatgt ctgcagggcc gaaaaagtgg caccgagtcg
      841 gtgcTTTTTT tccgcgggga tccagacaca attgcattca ttttatgttt caggttcagg
      901 gggaggtgtg ggaggttttt tcggatcctc tagagtcgac ctgcaggcat gcaagcttgg
      961 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1021 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1081 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1141 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1201 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1261 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1321 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1381 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1441 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1501 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1561 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1621 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1681 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1741 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1801 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1861 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1921 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1981 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2041 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2101 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2701 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2761 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2821 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2881 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2941 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3001 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3061 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3121 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3181 ggccctttAg tc    
//
