LOCUS       Exported               10088 bp ds-DNA     circular SYN 15-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="mutant attB recombination sequence for bxb1"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1952..2540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     region          2568..2605
                     /label=terminator
     CDS             complement(2606..2929)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     protein_bind    2949..2965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2973..3003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3018..3039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    complement(3051..3444)
                     /label=TRE-3G
     protein_bind    3204..3222
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    3240..3258
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    3276..3294
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    3312..3330
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    3348..3366
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    3384..3402
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    3420..3438
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     misc_feature    3469..5146
                     /label=pCAG
     misc_feature    5205..5948
                     /label=Tet-On(R) 3G
     CDS             5949..6002
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6003..6710
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             6735..6755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6759..6779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6783..6803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6874..7098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7132..7179
                     /label=FRT-F3 mutant
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
     rep_origin      7446..7901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8183..8287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8288..9148
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9319..9907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaata atgtcgagac ccatagagcc caccgcatcc
     1921 ccagcactag cgcggggagg cggcccaaag ggcggggagg cggcccaaag ggagatccga
     1981 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     2041 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     2101 atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc
     2161 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     2221 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     2281 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     2341 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     2401 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     2461 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     2521 ttgtaatcca gaggttgatt gcggccgctt ccgcgttata ggcttaattt gttcaaaaaa
     2581 aagcccgctc attaggcggg ctgggctatg cggcatcaga gcagattgta ctgagagtgc
     2641 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     2701 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2761 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2821 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     2881 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     2941 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3001 aaagcctggg gtgcctaatg agtgagctaa ctcacattag cggccgccat ggtggcgaat
     3061 tcttaagttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg
     3121 gaattgctcc aggcgatctg acggttcact aaacgagctc tgcttttata ggcgcccacc
     3181 gtacacgcct aaagcttata cgttctctat cactgatagg gagtaaactg gatatacgtt
     3241 ctctatcact gatagggagt aaactgtaga tacgttctct atcactgata gggagtaaac
     3301 tggtcatacg ttctctatca ctgataggga gtaaactcct tatacgttct ctatcactga
     3361 tagggagtaa agtctgcata cgttctctat cactgatagg gagtaaactc ttcatacgtt
     3421 ctctatcact gatagggagt aaaccttaat taactagtca ttaagctcga cattgattat
     3481 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3541 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3601 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3661 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3721 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3781 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3841 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     3901 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     3961 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     4021 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     4081 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg agtcgctgcg
     4141 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     4201 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     4261 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     4321 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     4381 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     4441 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     4501 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     4561 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     4621 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     4681 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     4741 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     4801 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     4861 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     4921 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     4981 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     5041 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     5101 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     5161 gctggttatt gtgctgtctc atcattttgg caaagaatgt cgacatgagc cggctggaca
     5221 agagcaaagt gatcaacagc gccctggaac tgctgaacgg cgtgggcatc gagggcctga
     5281 ccacccggaa gctggcccag aaactgggcg tggaacagcc caccctgtac tggcacgtga
     5341 agaacaagcg ggccctgctg gacgccctgc ccatcgagat gctggaccgg caccacaccc
     5401 acagctgccc tctggaaggc gagagctggc aggacttcct gcggaacaac gccaagagct
     5461 acagatgcgc cctgctgagc caccgggacg gcgccaaagt gcacctgggc accagaccca
     5521 ccgagaagca gtacgagaca ctggaaaacc agctggcctt cctgtgccag cagggcttca
     5581 gcctggaaaa cgccctgtac gccctgagcg ccgtgggcca cttcaccctg ggctgcgtgc
     5641 tggaagaaca ggaacaccag gtcgccaaag aggaaagaga gacacccacc accgacagca
     5701 tgccccccct gctgaagcag gccatcgagc tgttcgacag acagggcgcc gagcccgcct
     5761 tcctgttcgg cctggaactg atcatctgcg gcctcgagaa gcagctgaag tgcgagagcg
     5821 gcggacccac cgacgccctg gacgacttcg acctggacat gctgcccgcc gatgccctgg
     5881 atgattttga tctggatatg ctccctgctg acgctctcga tgacttcgat ctcgatatgc
     5941 tgccaggcga gggcagggga agtcttctaa catgcgggga cgtggaggaa aatcccggcc
     6001 ccgtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg
     6061 gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
     6121 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
     6181 tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc
     6241 ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg
     6301 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct
     6361 acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa
     6421 cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca
     6481 gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac gccaagacca
     6541 catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact
     6601 acagactgga aagaatcaag gaggccaaca acgagacata cgtcgagcag cacgaggtgg
     6661 cagtggccag atactgcgac ctccctagca aactggggca caagcttaat tccggactca
     6721 gatctcgagc tgatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag
     6781 atccaaaaaa gaagagaaag gtaggatcca ccggatctag ataactgatc ataatcagcc
     6841 ataccacatt tgaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6901 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6961 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7021 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7081 atgcggtggg ctctatggct agccgctgca agaactcttc ctcacggatc cgaagttcct
     7141 attccgaagt tcctattctt caaatagtat aggaacttca gcgctcactg gccgtcgttt
     7201 tacaaagggc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7261 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7321 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     7381 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     7441 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7501 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7561 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7621 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     7681 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7741 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     7801 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7861 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     7921 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     7981 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8041 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     8101 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8161 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8221 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8281 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8341 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8401 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8461 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8521 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8581 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8641 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8701 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8761 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8821 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8881 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8941 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9001 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9061 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9121 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9181 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9241 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9421 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     9481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9961 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10021 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10081 agcggaag
//
