LOCUS       Exported               10590 bp ds-DNA     circular SYN 15-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="mutant attB recombination sequence for bxb1"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1925..2251
                     /label=8xDBM
     promoter        2406..2436
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             2480..2803
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     region          2804..2841
                     /label=terminator
     misc_feature    2869..3457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    3590..5183
                     /label=pCAG
     regulatory      5245..5250
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5261..7375
                     /codon_start=1
                     /label=synZiFTR
                     /translation="MSRPGERPFQCRICMRNFSRRHGLDRHTRTHTGEKPFQCRICMRN
                     FSDHSSLKRHLRTHTGSQKPFQCRICMRNFSVRHNLTRHLRTHTGEKPFQCRICMRNFS
                     DHSNLSRHLKTHTGSQKPFQCRICMRNFSQRSSLVRHLRTHTGEKPFQCRICMRNFSES
                     GHLKRHLRTHLRGSTCRDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPGGLEG
                     GGGSGGTEDVVCCHSIYGKKKGDIDTYRYIGSSGTGCVVIVGRIVLSGSGTSAPITAYA
                     QQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWAVYHGAGTRTIAS
                     PKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGS
                     LLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRSPVF
                     TDNSSPPAVTLTHPITKIDREVLYQEFDEMEECSQHYPYDVPDYAGGGGSGGTDEFPTM
                     VFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPA
                     PKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPV
                     APHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFS
                     ALLSQISSQL"
     misc_feature    5261..5800
                     /label=ZF10
     CDS             5801..5866
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5873..5893
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5894..6742
                     /label=NS3
     CDS             6743..6769
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6995..7366
                     /label=p65
     polyA_signal    7376..7600
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7634..7681
                     /label=FRT-F3 mutant
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
     rep_origin      7948..8403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8685..8789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8790..9650
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9821..10409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aagcggacga ttgagtaaca ctgtatctag
     1921 tctacctaca gggctcggcg tagccgatgt cgcgcggata gcgtcaacct cttcggcgta
     1981 gccgatgtcg cgcaatcagt tctcccgagg tcggcgtagc cgatgtcgcg cagagccgtg
     2041 cttccaatac ggcgtagccg atgtcgcgcc gggtttcgta acaatcgcat gaggattcgc
     2101 aacgccttcg gcgtagccga tgtcgcgctc ccgtctcagt aaaggtcggc gtagccgatg
     2161 tcgcgcaatc ggactgcctt cgtacggcgt agccgatgtc gcgcgtatca gtcgcctcgg
     2221 aacggcgtag ccgatgtcgc gcattcgtaa gaggctcact ctcccttaca cggagtggat
     2281 aggatcctct agagggtata taatgggggc cactagtcta ctaccagagc tcatcgctag
     2341 cgctaccgga tcgccaccat ggcggccgct aatgtgagtt agctcactca ttaggcaccc
     2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac
     2521 tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
     2581 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2641 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2701 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2761 catatggtgc actctcagta caatctgctc tgatgccgca tagcccagcc cgcctaatga
     2821 gcgggctttt ttttgaacaa attaagccta taacgcggaa gcggccgcaa tcaacctctg
     2881 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2941 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3001 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3061 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3121 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3181 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3241 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3301 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3361 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3421 cagacgagtc ggatctccct ttgggccgcc tccccgccct ttgggccgcc tccccgcgct
     3481 agtgctgggg atgcggtggg ctctatgggt ctcgacattg attattgact agttattaat
     3541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     3901 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     3961 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg
     4021 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     4081 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     4141 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc
     4201 ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     4261 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     4321 ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc
     4381 gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     4441 cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     4501 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg
     4561 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc
     4621 ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     4681 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     4741 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     4801 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     4861 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     4921 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     4981 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     5041 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     5101 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     5161 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     5221 attttggcaa agaattccga tcgcgccacc tgccgccacc atgtctagac ccggagagcg
     5281 cccattccag tgtcggattt gcatgcggaa cttttcgaga agacacggcc tggacagaca
     5341 tacccgtact catacaggtg aaaaaccctt tcagtgtcgg atctgtatgc gaaatttctc
     5401 cgaccacagc agcctgaaga gacatctacg tacccacacc ggcagccaga agccatttca
     5461 gtgtcggatc tgtatgcgga acttctccgt gagacacaac ctgaccagac atctacgtac
     5521 gcacaccgga gagaagccat tccaatgccg aatatgcatg cgcaacttca gtgaccacag
     5581 caacctgagc agacacctaa aaacccacac cggttcccag aagccatttc agtgtcggat
     5641 ctgtatgcgg aacttctccc agcgcagcag cctggtgaga catctacgta cgcacaccgg
     5701 agagaagcca ttccaatgcc gaatatgcat gcgcaacttc agtgagagcg gccacctgaa
     5761 gagacacctg cgtacgcacc tgaggggatc cacctgcagg gactacaaag accatgacgg
     5821 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa
     5881 aaagaggaag gtgggcattc acggggtgcc gggtggactc gagggaggcg gtggaagcgg
     5941 cggtaccgag gacgtggtgt gctgccactc aatctacggc aagaagaagg gtgatatcga
     6001 cacctaccga tacataggct cttccgggac aggctgcgtg gtcatagtgg gcaggatcgt
     6061 cttgtccgga tccggcacta gtgcgcccat cacggcgtac gcccagcaga cgagaggcct
     6121 cctagggtgt ataatcacca gcctgactgg ccgggacaaa aaccaagtgg agggtgaggt
     6181 ccagatcgtg tcaactgcta cccaaacctt cctggcaacg tgcatcaatg gggtatgctg
     6241 ggcagtctac cacggggccg gaacgaggac catcgcatca cccaagggtc ctgtcatcca
     6301 gatgtatacc aatgtggacc aagaccttgt gggctggccc gctcctcaag gttcccgctc
     6361 attgacaccc tgtacctgcg gctcctcgga cctttacctg gtcacgaggc acgccgatgt
     6421 cattcccgtg cgccggcgag gtgatagcag gggtagcctg ctttcgcccc ggcccatttc
     6481 ctacttgaaa ggctcctcgg ggggtccgct gttgtgcccc gcgggacacg ccgtgggcct
     6541 attcagggcc gcggtgtgca cccgtggagt ggctaaagcg gtggacttta tccctgtgga
     6601 gaacctagag acaaccatga gatccccggt gttcacggac aactcctctc caccagcagt
     6661 caccctgacg cacccaatca ccaaaatcga tagggaggtt ctctaccagg agttcgatga
     6721 gatggaagag tgctctcagc actatcccta cgatgtgccc gattacgctg gaggcggtgg
     6781 aagcggcggt accgatgagt ttcccaccat ggtgtttcct tctgggcaga tcagccaggc
     6841 ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc ctgcccctgc
     6901 tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc tagccccagg
     6961 ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg aaggaacgct
     7021 gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct tgcttggcaa
     7081 cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg agtttcagca
     7141 gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc tgatggagta
     7201 ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc cagctcctgc
     7261 tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag acttctcctc
     7321 cattgcggac atggacttct cagccctgct gagtcagatc agctcccaat tgtaactgtg
     7381 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7441 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7501 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7561 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg ctagccgctg caagaactct
     7621 tcctcacgga tccgaagttc ctattccgaa gttcctattc ttcaaatagt ataggaactt
     7681 cagcgctcac tggccgtcgt tttacaaagg gcgaattcac tggccgtcgt tttacaacgt
     7741 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7801 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7861 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7921 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     7981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8161 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8281 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     8401 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     8461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     8521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     8581 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8641 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9961 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10441 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10501 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10561 gcagcgagtc agtgagcgag gaagcggaag
//
