LOCUS       Exported               10249 bp ds-DNA     circular SYN 16-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="mutant attB recombination sequence for bxb1"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1897..3574
                     /label=pCAG
     misc_feature    3633..4376
                     /label=Tet-On(R) 3G
     CDS             4377..4430
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4431..5138
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             5163..5183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5187..5207
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5211..5231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5302..5524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5566..6154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6158..6853)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     misc_feature    complement(6854..7247)
                     /label=TRE-3G
     protein_bind    7007..7025
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7043..7061
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7079..7097
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7115..7133
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7151..7169
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7187..7205
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7223..7241
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    7293..7340
                     /label=FRT-F3 mutant
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
     rep_origin      7607..8062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8344..8448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8449..9309
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9480..10068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aagctcgaca ttgattattg actagttatt
     1921 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     1981 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2041 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2101 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2161 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2221 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     2281 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     2341 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
     2401 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     2461 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2521 ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg
     2581 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     2641 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     2701 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     2761 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     2821 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     2881 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     2941 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     3001 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     3061 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     3121 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     3181 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     3241 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     3301 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     3361 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     3421 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     3601 gctgtctcat cattttggca aagaatgtcg acatgagccg gctggacaag agcaaagtga
     3661 tcaacagcgc cctggaactg ctgaacggcg tgggcatcga gggcctgacc acccggaagc
     3721 tggcccagaa actgggcgtg gaacagccca ccctgtactg gcacgtgaag aacaagcggg
     3781 ccctgctgga cgccctgccc atcgagatgc tggaccggca ccacacccac agctgccctc
     3841 tggaaggcga gagctggcag gacttcctgc ggaacaacgc caagagctac agatgcgccc
     3901 tgctgagcca ccgggacggc gccaaagtgc acctgggcac cagacccacc gagaagcagt
     3961 acgagacact ggaaaaccag ctggccttcc tgtgccagca gggcttcagc ctggaaaacg
     4021 ccctgtacgc cctgagcgcc gtgggccact tcaccctggg ctgcgtgctg gaagaacagg
     4081 aacaccaggt cgccaaagag gaaagagaga cacccaccac cgacagcatg ccccccctgc
     4141 tgaagcaggc catcgagctg ttcgacagac agggcgccga gcccgccttc ctgttcggcc
     4201 tggaactgat catctgcggc ctcgagaagc agctgaagtg cgagagcggc ggacccaccg
     4261 acgccctgga cgacttcgac ctggacatgc tgcccgccga tgccctggat gattttgatc
     4321 tggatatgct ccctgctgac gctctcgatg acttcgatct cgatatgctg ccaggcgagg
     4381 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggcccc gtgtctaagg
     4441 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     4501 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     4561 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     4621 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     4681 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     4741 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     4801 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     4861 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     4921 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
     4981 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     5041 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     5101 actgcgacct ccctagcaaa ctggggcaca agcttaattc cggactcaga tctcgagctg
     5161 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga
     5221 agagaaaggt aggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     5281 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5341 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5401 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5461 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5521 ctatcaccgc atccccagca ctagcgcggg gaggcggccc aaagggcggg gaggcggccc
     5581 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
     5641 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
     5701 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct
     5761 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
     5821 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
     5881 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
     5941 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     6001 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     6061 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     6121 atctttcaca aattttgtaa tccagaggtt gattttactt gtacagctcg tccatgccgc
     6181 cggtggagtg gcggccctcg gagcgttcgt actgttccac cacggtgtag tcctcgttgt
     6241 gggaggtgat gtccaacttg cggtcgacgt tgtaggcgcc gggcatctgc acgggcttct
     6301 tggccttgta ggtggtcttg aagtccgcca ggtagcggcc gccgtccttc aggcgcaggg
     6361 ccatcttaat gtcgcccttc agcacgccgt cctcggggta caaccgctcg gtggacgctt
     6421 cccagcccat tgtcttcttc tgcattacgg ggccgtcagg agggaagttg gtgccgcgga
     6481 gcttcacctt gtagatcagg gtgccgtcct ccagggaggt gtcctgggtc acggtcacgg
     6541 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggactgct
     6601 tatagtagtc ggggatgtcg gcggggtgct tggtgaaggc cctggagccg tacatgaact
     6661 gaggggacag gatgtcccag gagaagggca gggggccacc cttggtcacc ttcagcttgg
     6721 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
     6781 cgttcatgga gccctccatg tgcaccttga accgcatgaa ctccttgatc actgcctcgc
     6841 ccttgctcac catggtggcg aattcttaag tttacgaggg taggaagtgg tacggaaagt
     6901 tggtataaga caaaagtgtt gtggaattgc tccaggcgat ctgacggttc actaaacgag
     6961 ctctgctttt ataggcgccc accgtacacg cctaaagctt atacgttctc tatcactgat
     7021 agggagtaaa ctggatatac gttctctatc actgataggg agtaaactgt agatacgttc
     7081 tctatcactg atagggagta aactggtcat acgttctcta tcactgatag ggagtaaact
     7141 ccttatacgt tctctatcac tgatagggag taaagtctgc atacgttctc tatcactgat
     7201 agggagtaaa ctcttcatac gttctctatc actgataggg agtaaacctt aattaactag
     7261 tcgccgctgc aagaactctt cctcacggat ccgaagttcc tattccgaag ttcctattct
     7321 tcaaatagta taggaacttc agcgctcact ggccgtcgtt ttacaaaggg cgaattcact
     7381 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7441 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7501 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     7561 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     7621 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7681 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7741 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7801 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     7861 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7921 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     7981 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8041 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     8101 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8161 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8221 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     8281 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8341 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8401 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8461 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8521 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8581 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8641 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8701 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8761 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8821 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8881 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8941 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9001 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9061 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9121 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9181 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9241 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9301 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9361 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9421 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9481 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9541 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9601 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     9661 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9721 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9781 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9841 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9901 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9961 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10021 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10081 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10141 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10201 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
