LOCUS       Exported               10251 bp ds-DNA     circular SYN 16-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="mutant attB recombination sequence for bxb1"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1894..2287
                     /label=TRE-3G
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1936..1954
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1972..1990
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2008..2026
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2044..2062
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2080..2098
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     CDS             2288..2983
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     misc_feature    2987..3575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    3632..5309
                     /label=pCAG
     misc_feature    5368..6111
                     /label=Tet-On(R) 3G
     CDS             6112..6165
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6166..6873
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             6898..6918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6922..6942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6946..6966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7037..7261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7295..7342
                     /label=FRT-F3 mutant
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
     rep_origin      7609..8064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8346..8450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8451..9311
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9482..10070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aaggtttact ccctatcagt gatagagaac
     1921 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     1981 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     2041 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     2101 gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt
     2161 gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac
     2221 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa cttaagaatt
     2281 cgccaccatg gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca
     2341 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2401 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc
     2461 ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacca agcaccccgc
     2521 cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2581 gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac
     2641 cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca
     2701 gaagaagaca atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct
     2761 gaagggcgac attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt
     2821 caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg
     2881 caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc
     2941 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaaatc aacctctgga
     3001 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3061 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3121 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3181 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     3241 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3301 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3361 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     3421 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3481 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3541 gacgagtcgg atctcccttt gggccgcctc cccgcccttt gggccgcctc cccgcgctag
     3601 tgctggggat gcggtgggct ctatgggtct cgacattgat tattgactag ttattaatag
     3661 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3721 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3781 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3841 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3901 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3961 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     4021 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     4081 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
     4141 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     4201 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     4261 ccctataaaa agcgaagcgc gcggcgggcg gggagtcgct gcgacgctgc cttcgccccg
     4321 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     4381 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     4441 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     4501 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     4561 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     4621 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     4681 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     4741 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     4801 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     4861 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     4921 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     4981 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     5041 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     5101 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     5161 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     5221 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     5281 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     5341 ctcatcattt tggcaaagaa tgtcgacatg agccggctgg acaagagcaa agtgatcaac
     5401 agcgccctgg aactgctgaa cggcgtgggc atcgagggcc tgaccacccg gaagctggcc
     5461 cagaaactgg gcgtggaaca gcccaccctg tactggcacg tgaagaacaa gcgggccctg
     5521 ctggacgccc tgcccatcga gatgctggac cggcaccaca cccacagctg ccctctggaa
     5581 ggcgagagct ggcaggactt cctgcggaac aacgccaaga gctacagatg cgccctgctg
     5641 agccaccggg acggcgccaa agtgcacctg ggcaccagac ccaccgagaa gcagtacgag
     5701 acactggaaa accagctggc cttcctgtgc cagcagggct tcagcctgga aaacgccctg
     5761 tacgccctga gcgccgtggg ccacttcacc ctgggctgcg tgctggaaga acaggaacac
     5821 caggtcgcca aagaggaaag agagacaccc accaccgaca gcatgccccc cctgctgaag
     5881 caggccatcg agctgttcga cagacagggc gccgagcccg ccttcctgtt cggcctggaa
     5941 ctgatcatct gcggcctcga gaagcagctg aagtgcgaga gcggcggacc caccgacgcc
     6001 ctggacgact tcgacctgga catgctgccc gccgatgccc tggatgattt tgatctggat
     6061 atgctccctg ctgacgctct cgatgacttc gatctcgata tgctgccagg cgagggcagg
     6121 ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gccccgtgtc taagggcgaa
     6181 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     6241 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     6301 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     6361 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     6421 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     6481 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     6541 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     6601 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     6661 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     6721 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     6781 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     6841 gacctcccta gcaaactggg gcacaagctt aattccggac tcagatctcg agctgatcca
     6901 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga
     6961 aaggtaggat ccaccggatc tagataactg atcataatca gccataccac atttgaaacc
     7021 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7081 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7141 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7201 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7261 gctagccgct gcaagaactc ttcctcacgg atccgaagtt cctattccga agttcctatt
     7321 cttcaaatag tataggaact tcagcgctca ctggccgtcg ttttacaaag ggcgaattca
     7381 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7441 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7501 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     7561 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     7621 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7681 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7741 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7801 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     7861 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7921 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     7981 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8041 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     8101 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8161 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     8221 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     8281 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8341 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8401 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8461 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8521 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8581 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8641 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8701 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8761 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8821 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8881 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8941 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9001 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9061 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9121 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9181 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9241 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9301 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9361 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9421 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9481 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9541 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9601 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     9661 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9721 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9781 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9841 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9901 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9961 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10021 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10081 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10141 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10201 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
