LOCUS       Exported               10420 bp ds-DNA     circular SYN 13-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Richard Davis
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     misc_recomb     1754..1795
                     /label=attB
                     /note="from myobacteriumphage bxb1 (Thomson et al 2012)"
     protein_bind    1802..1835
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1875..2268
                     /label=TRE-3G
     protein_bind    1881..1899
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1917..1935
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1953..1971
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    1989..2007
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2025..2043
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2061..2079
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     protein_bind    2097..2115
                     /gene="tetO"
                     /label=tet operator
                     /label=tet\operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/vntifkey=31"
     regulatory      2263..2268
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2269..3057
                     /codon_start=1
                     /product="Mouse Ngn2 "
                     /label=mNgn2
                     /translation="MFVKSETLELKEEEEVLMLLGSASPASATLTPMSSSADEEEDEEL
                     RRPGSARGQRGAEAGQGVQGSPASGAGGCRPGRLLGLMHECKRRPSRSRAVSRGAKTAE
                     TVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWA
                     LTETLRLADHCAGAGGLQGALFTEAVLLSPGAALGASGDSPSPPSSWSCTNSPASSSNS
                     TSPYSCTLSPASPGSDVDYWQPPPPEKHRYAPHLPLARDCI"
     CDS             3097..3123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3133..3159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3169..3195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3206..3794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    3851..5528
                     /label=pCAG
     misc_feature    5587..6330
                     /label=Tet-On(R) 3G
     CDS             6331..6384
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6385..7092
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             7117..7137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7141..7161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7165..7185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7256..7480
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7509..7541
                     /label=lox251
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     rep_origin      7778..8233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8515..8619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8620..9480
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9651..10239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggtctccg tcgtcaggat catccggatc
     1801 cataacttcg tatagcatac attatacgaa gttatcatga tattcggcaa gcaggcatcg
     1861 actagttaat taaggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     1921 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     1981 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     2041 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     2101 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
     2161 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
     2221 aactttccgt accacttcct accctcgtaa acttaagaat tcgccaccat gttcgtcaaa
     2281 tctgagactc tggagttgaa ggaggaagag gaggtactga tgctgctggg ctcggcttcc
     2341 ccggcctcgg cgaccctgac cccgatgtcc tccagcgcgg acgaggagga ggacgaggag
     2401 ctgcgccggc cgggctccgc gcgtgggcag cgtggagcgg aagccgggca gggggtgcag
     2461 ggcagtccgg cgtcgggtgc cgggggttgc cggccagggc ggctgctggg cctgatgcac
     2521 gagtgcaagc gtcgcccgtc gcgctcacgg gccgtctccc gaggtgccaa gacggcggag
     2581 acggtgcagc gcatcaagaa gacccgcagg ctcaaggcca acaaccgcga gcgcaaccgc
     2641 atgcacaacc taaacgccgc gctggacgcg ctgcgcgagg tgctgcccac cttccccgag
     2701 gatgccaagc tcacgaagat cgagacgctg cgcttcgccc acaattacat ctgggcgctc
     2761 accgagactc tgcgcctggc ggaccactgc gccggcgccg gtggcctcca gggggcgctc
     2821 ttcacggagg cggtgctcct gagcccggga gctgcgctcg gcgccagcgg ggacagccct
     2881 tctccacctt cctcctggag ctgcaccaac agcccggcgt catcctccaa ctccacgtcc
     2941 ccatacagct gcactttatc gcccgctagc cccgggtcag acgtggacta ctggcagccc
     3001 ccacctccgg agaagcatcg ttatgcgcct cacctgcccc tcgccaggga ctgtatcggg
     3061 tctggctccg gatcaagtgg tggcagcgga tcaggatatc catacgatgt accagactat
     3121 gcgggatcgg gttaccccta cgacgtcccc gactacgccg gctccggcta cccctacgac
     3181 gtccctgatt atgcttagcg gccgcaatca acctctggat tacaaaattt gtgaaagatt
     3241 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3301 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3361 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3421 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3481 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3541 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3601 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3661 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3721 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3781 ggccgcctcc ccgccctttg ggccgcctcc ccgcgctagt gctggggatg cggtgggctc
     3841 tatgggtctc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     3901 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     3961 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4021 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4081 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4141 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4201 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     4261 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     4321 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     4381 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     4441 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     4501 cggcgggcgg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct
     4561 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     4621 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     4681 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     4741 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     4801 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     4861 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     4921 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     4981 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     5041 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
     5101 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc
     5161 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     5221 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     5281 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     5341 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     5401 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     5461 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     5521 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     5581 gtcgacatga gccggctgga caagagcaaa gtgatcaaca gcgccctgga actgctgaac
     5641 ggcgtgggca tcgagggcct gaccacccgg aagctggccc agaaactggg cgtggaacag
     5701 cccaccctgt actggcacgt gaagaacaag cgggccctgc tggacgccct gcccatcgag
     5761 atgctggacc ggcaccacac ccacagctgc cctctggaag gcgagagctg gcaggacttc
     5821 ctgcggaaca acgccaagag ctacagatgc gccctgctga gccaccggga cggcgccaaa
     5881 gtgcacctgg gcaccagacc caccgagaag cagtacgaga cactggaaaa ccagctggcc
     5941 ttcctgtgcc agcagggctt cagcctggaa aacgccctgt acgccctgag cgccgtgggc
     6001 cacttcaccc tgggctgcgt gctggaagaa caggaacacc aggtcgccaa agaggaaaga
     6061 gagacaccca ccaccgacag catgcccccc ctgctgaagc aggccatcga gctgttcgac
     6121 agacagggcg ccgagcccgc cttcctgttc ggcctggaac tgatcatctg cggcctcgag
     6181 aagcagctga agtgcgagag cggcggaccc accgacgccc tggacgactt cgacctggac
     6241 atgctgcccg ccgatgccct ggatgatttt gatctggata tgctccctgc tgacgctctc
     6301 gatgacttcg atctcgatat gctgccaggc gagggcaggg gaagtcttct aacatgcggg
     6361 gacgtggagg aaaatcccgg ccccgtgtct aagggcgaag agctgattaa ggagaacatg
     6421 cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag
     6481 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
     6541 cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc
     6601 atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca
     6661 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
     6721 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac
     6781 ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc
     6841 gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat
     6901 ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg
     6961 cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc
     7021 tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg
     7081 cacaagctta attccggact cagatctcga gctgatccaa aaaagaagag aaaggtagat
     7141 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaggatc caccggatct
     7201 agataactga tcataatcag ccataccaca tttgaaaccc gctgatcagc ctcgactgtg
     7261 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7321 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7381 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7441 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg ctagccgctg caagaactct
     7501 tcctcacgat aacttcgtat aggagacttt atacgaagtt aagcgctcac tggccgtcgt
     7561 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7621 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7681 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     7741 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     7801 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7861 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7921 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7981 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8041 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8101 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     8161 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8221 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8281 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     8341 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     8401 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8461 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9781 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
