LOCUS       Exported               13677 bp ds-DNA     circular SYN 07-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(622..1157)
                     /label=Tol2 3' End
     misc_feature    complement(622..638)
                     /label=3' Tol2 ITR
     primer_bind     1169..1185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1205..1225
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     1253..2664
                     /label=dr Ubiquitin promoter
     exon            2665..2711
                     /label=Ubiquitin Exon 1 (non-coding)
     intron          2712..4737
                     /label=Ubiquiin intron 1
     protein_bind    4762..4786
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4789..6750
                     /codon_start=1
                     /label=lexA Transactivator
                     /label=lexA\Transactivator
                     /note="/vntifkey=21"
                     /translation="MAPKKKRKMKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGF
                     RSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPSSAGTEFPG
                     VDQKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIE
                     PDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYS
                     WMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQIPQEFVKLQV
                     SQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKGVVSSSQRFYQ
                     LTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAGSTPMEFQYLPDTDDRH
                     RIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSL
                     STINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLA
                     PGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSE
                     FQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDE
                     DFSSIADMDFSALLSQISS"
     CDS             4789..4812
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="MAPKKKRK"
     CDS             4813..5064
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA repressor DBD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     misc_feature    5086..5109
                     /label=synthetic filler
     CDS             5110..6378
                     /codon_start=1
                     /product="Human progesterone receptor binding domain"
                     /label=Progesterone Binding Domain
                     /translation="KKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLI
                     PPLINLLMSIEPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLH
                     IDDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMW
                     QIPQEFVKLQVSQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQK
                     GVVSSSQRFYQLTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAGSTPME
                     FQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKP
                     APQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSAL
                     AQAPAPVPVLAPGPPQAVAPPAPK"
     CDS             6379..6750
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA 
                     (NFkB), also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) Activation Domain
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     6780..6805
                     /label=oJDW XXX (LexOp FWD1)
     polyA_signal    6880..7001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(6917..6936)
                     /label=SV40pA REVSeq
     misc_feature    6998..7027
                     /label=Synthetic Filler DNA
     promoter        7028..7645
                     /label=lexA Operator
                     /label=lexA\Operator
                     /note="/vntifkey=21"
     misc_feature    7028..7274
                     /label=NOS terminator
                     /note="nopaline synthase terminator from Agrobacterium
                     tumefaciens (NOS terminator)"
     misc_feature    7338..7489
                     /label=Synthetic LexA operator
     misc_feature    7338..7372
                     /label=ColE1 Operator
                     /note="ColE1 operator sequences (Ebina et
                     al., 1983)"
     misc_feature    7374..7408
                     /label=ColE1 Operator
                     /note="ColE1 operator sequences (Ebina et
                     al., 1983).
                     
                     Ebina, Y., Takahara, Y., Kishi, F., Nakazawa, A., and 
                     Brent, R. (1983). LexA protein is a repressor of the
                     colicin E1 gene. J Biol Chem 258, 13258-61. 
                     "
     misc_feature    7416..7450
                     /label=ColE1 Operator
                     /note="ColE1 operator sequences (Ebina et
                     al., 1983)"
     misc_feature    7452..7486
                     /label=ColE1 Operator
                     /note="ColE1 operator sequences (Ebina et
                     al., 1983)"
     promoter        7571..7626
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(7621..7646)
                     /label=oJDW XXX (lexOP REV1)
     protein_bind    complement(7647..7671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7679..7699
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7718..7735)
                     /label=oJDW 124 (dr_Tol2_ATTR2_3'_REV)
     CDS             7745..8464
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7786..7804)
                     /label=EGFPN1R (Eurofins)
     primer_bind     complement(7790..7811)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8051..8070)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8398..8419
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8431..8449
                     /label=EGFPC1F (Eurofins)
     promoter        complement(8499..8517)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8500..8517)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8537..8554)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8543..8767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8769..8789)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    complement(8797..8813)
                     /label=M13 For -20
     polyA_signal    8829..8963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8833..9024
                     /label=SV40 late polyA
     misc_feature    8907..8926
                     /label=SV40pA REVSeq
     primer_bind     complement(9038..9063)
                     /label=oJDW XXX (LexOp FWD1)
     misc_feature    complement(9120..9139)
                     /label=SV40pA REVSeq
     promoter        9222..9239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9234..9257
                     /label=oJDW 124 (dr_Tol2_ATTR2_3'_REV)
     CDS             complement(9265..9984)
                     /codon_start=1
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9280..9298)
                     /label=EGFPC1F (Eurofins)
     misc_feature    complement(9310..9331)
                     /label=EGFP C FWD primer
     primer_bind     complement(9310..9331)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9659..9678
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9918..9939
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     9925..9943
                     /label=EGFPN1R (Eurofins)
     promoter        complement(9985..10883)
                     /label=cmlc2_promoter
                     /note="danio rerio"
     misc_feature    complement(10890..11406)
                     /label=Tol2 5' end
     primer_bind     complement(11154..11180)
                     /label=oJDW 160 (Tol2 5' arm REV_SEQ)
     misc_feature    complement(11388..11406)
                     /label=5' Tol2 ITR
     primer_bind     complement(11440..11456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11493..11511)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11532..11548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11556..11572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11580..11610)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11625..11646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11934..12522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12693..13553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13554..13658)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttggctcc gaattcgccc ttaaaactcg agaccagcaa
     1261 agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat tctgagtaag
     1321 ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata aatgataaca
     1381 aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc ctcaaacagt
     1441 gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa ttggttcaga
     1501 agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc tgattccaca
     1561 atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac agcactcact
     1621 agctgcccta tatattttat tatattttac atatattatt ttatttattt agctctgagt
     1681 gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta cctcaggctc
     1741 agggaatttg gaacaggttt ggtctgggtt tgtttcttta accatgcatg cttgttttca
     1801 actatggcaa cacagtcaca tgggacatta cagaaatgat ttgtcgatga catgcgactt
     1861 ttctttaata aagcgcaaag atcccaaaaa gcaaactttt aacaaaaatc atataattat
     1921 attttcaatc cagctttgta gcaactttgt gctgctgttc actcagcaac agatagtcag
     1981 tataaggtca gtgtgtctca aagcagtgcc atctgtttca cacattgcgt tctatatata
     2041 agtgtgctgg ttgacacgac actgtataag gcctaggcta aaacacaaac aatgtagaat
     2101 gacactgtgt tttttttgta aacaaatgtt gtttttggtt aaacatcttt gtgaaaacat
     2161 cctcctgtca tgtatttgct atattcaaat gttaaacccg tgcagaatag aacatataca
     2221 aaaaaaaaca acacaacaca tttttaaaca ttattaaata tcaagtattg ctggcagttc
     2281 tgtttctgtt ttacagtacc ctttgccaca gttctccgct tttcctggtc cagattccac
     2341 aagtctgatt caccaatagc aaagcgaata aacaaccaaa gcagccaatc actgcttgta
     2401 gactgtcctg cgagaccggc ccattccagc acattctgga aacttccttt atatgataat
     2461 tataaataca tttaaattat tgatacaaaa catgtaattc ctagaacata accatagcaa
     2521 tcattagttt tcagggtaat tatgtatttt taggatttga ctgcggaaag atctggtcat
     2581 gtgacgtctc atgaacgtca cggccctggg tttctataaa tacagtagga ctctcgacca
     2641 tcggcagatt tttcgaagaa gaagatcagt ttcaggagcc gtactgttcc gtttcaacgc
     2701 aaatattaac ggtaagagcg aatttcctag tttgttttca tgccattctt taaaaccata
     2761 gcgtattact ttaattatag taaactttcg ctttctttat tacaagagac gttttgtgtt
     2821 gattctccgc ggacattttc ggtcagacaa tcagaaaatg accgcggagg accagtaact
     2881 tgcattacac gtaagttaaa tcttcgtgta ttaaaatggt taggttgtta acgtcaaata
     2941 ggttaccgtg tttgcgtgtg atcaggttgg ttttgttaga tttttgtcag tatttttaat
     3001 ttatttgttt tagtttattt attttttttg ctgaatcata gtttgtgaac aaagaacccg
     3061 gatgttacat acagtacagc cgccatgtta cagagagtta taacttaatc attttaaaaa
     3121 taattttgcc ttacttttag tttgtcatgt tgagaaatga ggaaatgtta aaatgaggaa
     3181 atatccaatt aatttaatat atcaaaataa tccatgatta caatgcactg aactggagaa
     3241 aattaagatg ttttctagtg tcatgaaaca aatgtaagag atgtacattg tagatgtttt
     3301 atgtcaagaa ttggctagtt gatgcagcat actggcgata ctcagttgta ataacagtaa
     3361 cgttacatgt taatagacta ctgagtatgc tgttctgtct atgtatgctc tgtaagctac
     3421 gagaaggact tttttaaaca gtaaagggtg caatattttt acaaattgaa ttaaataaag
     3481 gctgtctatt aagtaatatg cttgatattt ttcttacttg atcggaaata agaaaaaata
     3541 taaacgttgt tgctctaaaa atcctagttc agtttagcca accacaaata cctttttgtt
     3601 cctccaacag tttttttttc ttctctataa tatttggcag tctatagtac tccaaatgtt
     3661 tccccacagt ctaactaatt ggtacagcca aaatcatgac acttattgca ataataattt
     3721 tggttcattg gcattgttga tagcctgtgc cactaatatg gtcgattgat catgcttcag
     3781 gaagaaaact atattgtttg atgtaagatt attaaatctt cacctgcttc cattacaaac
     3841 tattcccatc ttattgaatt ctggtatgtc ttaaaggatt agttcacttc ccaaatcaaa
     3901 atttacttag ccttttttca tccatgatcc ctttttttca tcattaatga agaaattgtt
     3961 tttgaaaaag tttcaagatt tttttctcta tattgtggag cttgttagtt taaaattcca
     4021 aaatgcaata tgtggcttca aatggttcta aatgatccca gtcaaggaat aacagtctta
     4081 tctaatgaaa ccattagacc tttaaaaata aaaaaaataa aagtatttat ttttaaatga
     4141 ctgagtgatt aagttgaatt tcagcgtttc cttactgtgt agaagtcctt ccttactggc
     4201 cccacccttt ggttctctgc caatctgcta cctaatgtaa tgttgtggaa cattattatt
     4261 ctttatttct taatttttta ttttttattt taaaacaatg taaactgcac agatgtgcag
     4321 tttgtttaaa atggccaatg ctttggaaat gcatgacata attagatttc atgatgcaca
     4381 aagccaaatc tcagagcttg tgcaaaatga gctatcattt cactaggtaa gaccctaaat
     4441 tttcatatag gatcatttgg acaattttgc tgcaggtaaa atgcattcta tagtccactg
     4501 tcagccattg ttttggatag tatttatttt tctctacaag tatagtcaat agttttctat
     4561 tattttaaag gtttgtaaca tttaagggtg accaaatgca aagtaaaatt tcattttcgg
     4621 gtgaactatc tcgtttaaca tgggagaagt gcaaaacata cattattggc tagaacattg
     4681 tagtattttt taaatggaaa tgtgtgattg ctaatcttac tttgaatttg tttacaggga
     4741 tcctttaagg gcgaattcga cccaagtttg tacaaaaaag caggctggat ggcacccaag
     4801 aagaagagga agatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt
     4861 gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg
     4921 ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt
     4981 gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg
     5041 ccgctggtag gtcgtgtggc tgccggtgaa ccgtcgagcg ccggtaccga attcccgggt
     5101 gtcgaccaga aaaagttcaa taaagtcaga gttgtgagag cactggatgc tgttgctctc
     5161 ccacagccag tgggcgttcc aaatgaaagc caagccctaa gccagagatt cactttttca
     5221 ccaggtcaag acatacagtt gattccacca ctgatcaacc tgttaatgag cattgaacca
     5281 gatgtgatct atgcaggaca tgacaacaca aaacctgaca cctccagttc tttgctgaca
     5341 agtcttaatc aactaggcga gaggcaactt ctttcagtag tcaagtggtc taaatcattg
     5401 ccaggttttc gaaacttaca tattgatgac cagataactc tcattcagta ttcttggatg
     5461 agcttaatgg tgtttggtct aggatggaga tcctacaaac acgtcagtgg gcagatgctg
     5521 tattttgcac ctgatctaat actaaatgaa cagcggatga aagaatcatc attctattca
     5581 ttatgcctta ccatgtggca gatcccacag gagtttgtca agcttcaagt tagccaagaa
     5641 gagttcctct gtatgaaagt attgttactt cttaatacaa ttcctttgga agggctacga
     5701 agtcaaaccc agtttgagga gatgaggtca agctacatta gagagctcat caaggcaatt
     5761 ggtttgaggc aaaaaggagt tgtgtcgagc tcacagcgtt tctatcaact tacaaaactt
     5821 cttgataact tgcatgatct tgtcaaacaa cttcatctgt actgcttgaa tacatttatc
     5881 cagtcccggg cactgagtgt tgaatttcca gaaatgatgt ctgaagttat tgctgggtcg
     5941 acgcccatgg aattccagta cctgccagat acagacgatc gtcaccggat tgaggagaaa
     6001 cgtaaaagga catatgagac cttcaagagc atcatgaaga agagtccttt cagcggaccc
     6061 accgaccccc ggcctccacc tcgacgcatt gctgtgcctt cccgcagctc agcttctgtc
     6121 cccaagccag caccccagcc ctatcccttt acgtcatccc tgagcaccat caactatgat
     6181 gagtttccca ccatggtgtt tccttctggg cagatcagcc aggcctcggc cttggccccg
     6241 gcccctcccc aagtcctgcc ccaggctcca gcccctgccc ctgctccagc catggtatca
     6301 gctctggccc aggccccagc ccctgtccca gtcctagccc caggccctcc tcaggctgtg
     6361 gccccacctg cccccaagcc cacccaggct ggggaaggaa cgctgtcaga ggccctgctg
     6421 cagctgcagt ttgatgatga agacctgggg gccttgcttg gcaacagcac agacccagct
     6481 gtgttcacag acctggcatc cgtcgacaac tccgagtttc agcagctgct gaaccagggc
     6541 atacctgtgg ccccccacac aactgagccc atgctgatgg agtaccctga ggctataact
     6601 cgcctagtga caggggccca gaggcccccc gacccagctc ctgctccact gggggccccg
     6661 gggctcccca atggcctcct ttcaggagat gaagacttct cctccattgc ggacatggac
     6721 ttctcagccc tgctgagtca gatcagctcc taaggcggcc ggccgcgact ctagatcata
     6781 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     6841 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     6901 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6961 cattctagtt gtggtttgtc caaactcatc aatgtatctt aagtttaaac gcgttaacaa
     7021 ttggaaatta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     7081 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     7141 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     7201 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattgat
     7261 cccccctcga cagcttgcat gccgcttggg ctgcaggtcg aggctaaaaa actaatcgca
     7321 ttatcatccc ctcgacgtac tgtacatata accactggtt ttatatacag cagtactgta
     7381 catataacca ctggttttat atacagcagt cgacgtactg tacatataac cactggtttt
     7441 atatacagca gtactgtaca tataaccact ggttttatat acagcagtcg aggtaagatt
     7501 agatatggat atgtatatgg atatgtatat ggtggtaatg ccatgtaata tgctcgactc
     7561 taggatcttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     7621 gctgaagcta gtcgactcta gcctcgaccc agctttcttg tacaaagtgg aaatggctcc
     7681 aaagaagaag cgtaaggtaa atcgatcgaa ttcaaggcct ctcgagcctc tagaaggtgg
     7741 cggaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     7801 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     7861 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     7921 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     7981 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     8041 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     8101 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     8161 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     8221 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     8281 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     8341 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     8401 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     8461 gtaagcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
     8521 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     8581 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     8641 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8701 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     8761 tctatggcca actttattat acatagttga taattcactg gccgtcgttt tacggtacca
     8821 tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     8881 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     8941 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     9001 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     9061 attatgatcc tctagatcag atctcttgtt tattgcagct tataatggtt acaaataaag
     9121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9181 gtccaaactc atcaatgtat cttatcatgt ctggatctac gtaatacgac tcactatagt
     9241 tctagaggct cgagaggggc cgctttactt gtacagctcg tccatgccga gagtgatccc
     9301 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     9361 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     9421 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     9481 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
     9541 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     9601 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     9661 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
     9721 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     9781 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     9841 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     9901 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     9961 cagctcctcg cccttgctca ccatggtcac tgtctgcttt gctgttggtc tgggctcctg
    10021 ggtcactggc ttactaatgg agtctttatg tatgaggact cttatcaatt gttcttctat
    10081 aaaggtctgc agtgtttctg ttcgtcccct acatggacac ccagagcctc ctaaatacag
    10141 gagccctgat aactgcacaa gtgctcagat tccagcaggg tggaaaatga gataaagtgt
    10201 gcagatgggg agggggacgt gaatgagaga tttgagggat gaaaaggatg gatgaacgca
    10261 ttgaaaatag cccctttcac aaaataatac cagtaaattg tcatataatt aatgaccaga
    10321 cctttacctg taaatgatgt gatgttcata tacagaagaa tgccaacaat ttacagatga
    10381 ttttacaaat tcttaatttt ccttttctaa atggatttgt tcacacatta tctcttcaaa
    10441 gcaattgaat tttctggaaa agattggctg tgtgtgtaaa aggtgctact gaggaattta
    10501 atgtgacatg gaatgaagca aacagcagcc taagacagga tggcaggaaa aatgtcacat
    10561 gtttaaaata agagtgaaac caacgacctg gacttataga gactgtgtgt ccttaggcaa
    10621 agctcttgta caaacagtgc ttgtgactgt gaaattcggg gtttgcctgg attgtgttaa
    10681 atgtgtgctt ggatgtatca taaaaaagaa tcggttctct gtatttgaca ttttctgtaa
    10741 tttatctaaa gttacaactg gctatgcctg atttttatca ctattgaatc aatcatttta
    10801 aagaatgtta agtctgctga tttggagagc tcatttacat ttattttgaa tgtctcttat
    10861 ttaacacaac tgatttaagc tttagatctg cgaagatacg gccacgggtg ctcttgatcc
    10921 tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt
    10981 ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg
    11041 tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt
    11101 catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca
    11161 tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa
    11221 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
    11281 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
    11341 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
    11401 cctctggttc ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg
    11461 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
    11521 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    11581 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    11641 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    11701 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    11761 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11821 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11881 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11941 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    12001 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    12061 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    12121 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    12181 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    12241 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    12301 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    12361 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    12421 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    12481 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12541 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    12601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    12661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    12721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    12781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    12841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    12901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    13021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    13081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    13141 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    13201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    13261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    13321 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    13381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    13441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    13501 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    13561 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    13621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
