LOCUS       Exported                9619 bp ds-DNA     circular SYN 06-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(807..826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2513..2737
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     mobile_element  2513..2737
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2804..3328)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="SSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFG
                     REFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRSEIY
                     IYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEV
                     MHFDIDPSTAT"
     promoter        complement(3507..3535)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   3875..4004
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(3897..3908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3947..3958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4018..4332
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4026..4044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4062..4080
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4097..4115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4133..4151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4169..4187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4204..4222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4240..4258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4260..4271
                     /label=pTight Hybrid Sequence
     primer_bind     4270..4279
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4277..4296
                     /label=oJDW 267
     primer_bind     4305..4329
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4357..4390
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    4441..4474
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     4475..4505
                     /label=oJDW 1399
     protein_bind    4497..4621
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4662..4967)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(5309..5968)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(6022..6052)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6077..6201)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6190..6226)
                     /label=oJDW 1398
     protein_bind    complement(6227..6260)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(6311..6344)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    6389..6977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6442..6462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(6442..6462)
                     /label=oJDW 257
     primer_bind     complement(6981..6992)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7010..7027)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7016..7127
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7275..7323
                     /note="synthetic polyadenylation signal"
     misc_feature    7337..7428
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7377..7396
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     7443..7483
                     /label=F-MluI-EFS
     primer_bind     7449..7462
                     /label=oJDW 1356 EFS F (SalI)
     promoter        7468..7679
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(7647..7687)
                     /label=R-BamHI-EFS
     primer_bind     complement(7661..7680)
                     /label=oJDW 1357 EFS R (SpeI)
     CDS             7692..7694
                     /codon_start=1
                     /label=Met
                     /translation="M"
     CDS             7695..7814
                     /codon_start=1
                     /gene="Mus musculus ornithine decarboxylase gene 
                     destablization sequence.
                     
                     http://www.jbc.org/content/278/14/12135.abstract
                     "
                     /product="COOH 37 amino acids of mODC"
                     /label=mODC DEST
                     /note="mm Odc1 destablization domain"
                     /translation="SHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     CDS             7824..8570
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8583..8599)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8584..8600)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8624..8643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8625..8643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8669..8803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8706..8725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8760..8779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8833..8973
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(8879..8890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8940..8951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    9110..9244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9147..9166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9201..9220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9273..9438
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     2461 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     2521 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2581 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2641 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2701 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     2761 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     2821 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     2881 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     2941 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     3001 ctgcgagatc atagatatag atctcactac gcggctgctc aaacctgggc agaacgtaag
     3061 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     3121 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     3181 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     3241 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     3301 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     3361 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     3421 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     3481 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     3541 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     3601 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     3661 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc
     3721 ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     3781 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     3841 gctatagttc tagtggttgg ctacagcttg catgcctgca ggcagctgcg cgctcgctcg
     3901 ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
     3961 agcgcgcaga gagggagtgg ccaactccat cactaggggt tcctgcggcc gcatctcgag
     4021 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     4081 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     4141 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     4201 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     4261 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     4321 cgtcagatcg ccaccggctc tagagtcgac tccggaataa cttcgtatag gatactttat
     4381 acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta
     4441 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccgctcgaga ggatcaacca
     4501 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     4561 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     4621 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
     4681 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
     4741 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
     4801 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
     4861 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
     4921 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
     4981 gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
     5041 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
     5101 gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
     5161 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat
     5221 acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc
     5281 tggcttttag taagccggat ccagatcttt acgccccgcc ctgccactca tcgcagtact
     5341 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     5401 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     5461 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     5521 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     5581 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     5641 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     5701 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     5761 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     5821 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     5881 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     5941 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
     6001 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
     6061 tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     6121 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     6181 agcttttttg tacaaacttg ttgatccttg aattcgaatc gctagcataa cttcgtataa
     6241 agtatcctat acgaagttat ttgccttaac ccagaaatta tcactgttat tctttagaat
     6301 ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag ttatggcgcc agcgatccac
     6361 cggatctaga taactgatcc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     6421 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6481 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6541 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6601 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6661 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6721 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6781 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6841 gcccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6901 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6961 ttgggccgcc tccccgcatc gataccgtcg actagagctc gctgatcagc ctcgactgtg
     7021 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7081 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7141 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7201 gacaatagca gactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt
     7261 attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     7321 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     7381 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc
     7441 cgacgcgtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     7501 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     7561 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     7621 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     7681 ggatccagct catgagccat ggcttcccgc cggaggtgga ggagcaggat gatggcacgc
     7741 tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc tgtgcttctg
     7801 ctaggatcaa tgtggaattc accatgtcta gactggacaa gagcaaagtc ataaactctg
     7861 ctctggaatt actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     7921 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     7981 atgccctggc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     8041 agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac
     8101 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     8161 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     8221 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag
     8281 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     8341 caattgagct gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa
     8401 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg
     8461 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     8521 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagc
     8581 atcgataccg tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
     8641 ttacgcgcgc agcggccgac catggcccaa cttgtttatt gcagcttata atggttacaa
     8701 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8761 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctccggac cacgtgcgga
     8821 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     8881 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     8941 cagtgagcga gcgagcgcgc agctgcctgc aggaagctgt aagcttgtcg agaagtacta
     9001 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     9061 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     9121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9241 gatctgatca ctgatatcgc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa
     9301 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     9361 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     9421 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
     9481 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa
     9541 atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt
     9601 gcagcctgaa tggcgaatg
//
