LOCUS       Exported                9502 bp ds-DNA     circular SYN 15-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    656..968
                     /label=PB 5'ITR
     repeat_region   complement(660..694)
                     /label=piggyBac 5' ITR
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1016..1330
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1024..1042
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1060..1078
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1095..1113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1131..1149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1167..1185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1202..1220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1238..1256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1258..1269
                     /label=pTight Hybrid Sequence
     primer_bind     1268..1277
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1303..1327
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1355..1388
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     primer_bind     1389..1408
                     /label=F-Flex-Seq-New
     protein_bind    1439..1472
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     1473..1503
                     /label=oJDW 1399
     protein_bind    1495..1519
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1499..1559
                     /label=oJDW 1018
     CDS             complement(1537..2247)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1540..1561
                     /label=oJDW 1371
     CDS             complement(2254..2637)
                     /codon_start=1
                     /label=H2A.F/Z
                     /translation="MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKTRTTSHG
                     RVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKAT
                     IAGGGVIPHIHKSLIGKKGQQKTA"
     primer_bind     complement(2618..2668)
                     /label=oJDW 1017 (P2A_AvrII_H2A_FWD)
     primer_bind     complement(2618..2668)
                     /label=oJDW 1017
     primer_bind     complement(2618..2637)
                     /label=oJDW 1370
     regulatory      2635..2642
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(2644..2700)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     2702..2735
                     /label=oJDW 1369
     CDS             complement(2710..4359)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=firefly luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2735..2766
                     /label=oJDW 1406
     primer_bind     3506..3533
                     /label=oJDW 1405
     primer_bind     4289..4309
                     /label=oJDW 1244 (Luciferase N-term REV)
     primer_bind     complement(4331..4377)
                     /label=oJDW 1368
     primer_bind     4336..4358
                     /label=oJDW 406
     regulatory      4356..4365
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(4378..4402)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4391..4427)
                     /label=oJDW 1398
     protein_bind    complement(4428..4461)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     primer_bind     complement(4498..4520)
                     /label=R-Flex-Seq-New
     protein_bind    complement(4512..4545)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4590..5178
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4643..4663)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(5182..5193)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5211..5228)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5217..5328
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5476..5524
                     /note="synthetic polyadenylation signal"
     misc_feature    5538..5629
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5578..5597
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     5644..5684
                     /label=F-MluI-EFS
     promoter        5650..5881
                     /label=EFS (EF-1-alpha core promoter)
     primer_bind     complement(5848..5888)
                     /label=R-BamHI-EFS
     CDS             5893..5895
                     /codon_start=1
                     /label=Start
                     /translation="M"
     CDS             5896..6015
                     /codon_start=1
                     /gene="Mus musculus ornithine decarboxylase gene 
                     destablization sequence.
                     
                     http://www.jbc.org/content/278/14/12135.abstract
                     "
                     /product="COOH 37 amino acids of mODC"
                     /label=mODC DEST
                     /note="mm Odc1 destablization domain"
                     /translation="SHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     CDS             6025..6771
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6784..6800)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6785..6801)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6825..6844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6826..6844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6870..7004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6907..6926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6961..6980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    complement(7061..7295)
                     /label=PB 3'ITR
     misc_feature    7228..7290
                     /label=PiggyBac 3' ITR
     promoter        complement(7314..7332)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7353..7369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7353..7369
                     /label=M13-REV-Inverse
     protein_bind    7377..7393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7401..7431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7446..7467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7755..8343)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8514..9374)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8544..8564)
                     /label=AmpR-REV
     promoter        complement(9375..9479)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9416..9435
                     /label=AmpP-FWD
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcgt cgacttaatt aacctaggat ttaaatgcgg ccgcacgcgc gtctcgagtt
     1021 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     1081 gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     1141 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     1201 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     1261 atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
     1321 tcagatcgcc accggctcta gagtcgactc cggaataact tcgtatagga tactttatac
     1381 gaagttatgc agaatggtag ctggattgta gctgctatta gcaatatgaa acctcttaat
     1441 aacttcgtat agcatacatt atacgaagtt atggcgcgcc gctcgagagg atcaaccact
     1501 ttgtacaaga aagctgggag atctctcgag ctatcattac ttgtacagct cgtccatgcc
     1561 gccggtggag tggcggccct cggcgcgctc gtactgttcc acgatggtgt agtcctcgtt
     1621 gtgggaggtg atgtccagct tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
     1681 cttggccttg taggtggtct tgacctcggc gtcgtagtgg ccgccgtcct tcagcttcag
     1741 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
     1801 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
     1861 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
     1921 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
     1981 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
     2041 ctgaggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt
     2101 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
     2161 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
     2221 gttgtcctcc tcgcccttgc tcaccatccc ccctgcggtt ttctgctggc ccttcttacc
     2281 aatcagagac ttgtggatgt gaggaatgac tcctcctcca gcaatggtgg ccttgataag
     2341 cgagtccagc tcctcgtctc cgcggatggc cagctgtaaa tgtctgggag tgattcgctt
     2401 caccttcaga tccttagaag cgtttccagc caactccagc acctcagcgg tgagatactc
     2461 caggatagca gcactgtaaa ccgcagctgt agctccgaca cggccatggc tggtggttcg
     2521 ggtcttcagg tgcctgtgga tacgacctac agggaactgc agaccagctc tctgagatct
     2581 ggagacggct ttcgtcttgg cttttccact gtctttacct gcttttccac ctgccatcct
     2641 aggtggcccg ggattctctt cgacatcccc tgcttgtttc aacagggaga agttagtggc
     2701 tccggtaccc acggcgatct ttccgccctt cttggccttt atgaggatct ctctgatttt
     2761 tcttgcgtcg agttttccgg taagaccttt cggtacttcg tccacaaaca caactcctcc
     2821 gcgcaacttt ttcgcggttg ttacttgact ggcgacgtaa tccacgatct ctttttccgt
     2881 catcgtcttt ccgtgctcca aaacaacaac ggcggcggga agttcaccgg cgtcatcgtc
     2941 gggaagacct gcgacacctg cgtcgaagat gttggggtgt tggagcaaga tggattccaa
     3001 ttcagcggga gccacctgat agcctttgta cttaatcaga gacttcaggc ggtcaacgat
     3061 gaagaagtgt tcgtcttcgt cccagtaagc tatgtctcca gaatgtagcc atccatcctt
     3121 gtcaatcaag gcgttggtcg cttccggatt gtttacataa ccggacataa tcataggacc
     3181 tctcacacac agttcgcctc tttgattaac gcccagcgtt ttcccggtat ccagatccac
     3241 aaccttcgct tcaaaaaatg gaacaacttt accgaccgcg cccggtttat catccccctc
     3301 gggtgtaatc agaatagctg atgtagtctc agtgagccca tatccttgcc tgatacctgg
     3361 cagatggaac ctcttggcaa ccgcttcccc gacttcctta gagaggggag cgccaccaga
     3421 agcaatttcg tgtaaattag ataaatcgta tttgtcaatc agagtgcttt tggcgaagaa
     3481 ggagaatagg gttggcacca gcagcgcact ttgaatcttg taatcctgaa ggctcctcag
     3541 aaacagctct tcttcaaatc tatacattaa gacgactcga aatccacata tcaaatatcc
     3601 gagtgtagta aacattccaa aaccgtgatg gaatggaaca acacttaaaa tcgcagtatc
     3661 cggaatgatt tgattgccaa aaataggatc tctggcatgc gagaatctca cgcaggcagt
     3721 tctatgaggc agagcgacac ctttaggcag accagtagat ccagaggagt tcatgatcag
     3781 tgcaattgtc ttgtccctat cgaaggactc tggcacaaaa tcgtattcat taaaaccggg
     3841 aggtagatga gatgtgacga acgtgtacat cgactgaaat ccctggtaat ccgttttaga
     3901 atccatgata ataatttttt ggatgattgg gagctttttt tgcacgttca aaattttttg
     3961 caaccccttt ttggaaacga acaccacggt aggctgcgaa atgcccatac tgttgagcaa
     4021 ttcacgttca ttataaatgt cgttcgcggg cgcaactgca actccgataa ataacgcgcc
     4081 caacaccggc ataaagaatt gaagagagtt ttcactgcat acgacgattc tgtgatttgt
     4141 attcagccca tatcgtttca tagcttctgc caaccgaacg gacatttcga agtactcagc
     4201 gtaagtgatg tccacctcga tatgtgcatc tgtaaaagca attgttccag gaaccagggc
     4261 gtatctcttc atagccttat gcagttgctc tccagcggtt ccatcttcca gcggatagaa
     4321 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggcactag tggatccagc
     4381 ctgctttttt gtacaaactt gttgatcctt gaattcgaat cgctagcata acttcgtata
     4441 aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa
     4501 tggtgcaaag aataacttcg tataatgtat gctatacgaa gttatggcgc cagcgatcca
     4561 ccggatctag ataactgatc cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4621 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4681 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4741 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4801 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4861 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4921 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4981 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5041 cgcccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5101 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5161 tttgggccgc ctccccgcat cgataccgtc gactagagct cgctgatcag cctcgactgt
     5221 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5281 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5341 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5401 agacaatagc agactagtac gtctctcaag gataagtaag taatattaag gtacgggagg
     5461 tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     5521 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     5581 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctg gcctaactgg
     5641 ccgacgcgtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5701 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     5761 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5821 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5881 gggatccagc tcatgagcca tggcttcccg ccggaggtgg aggagcagga tgatggcacg
     5941 ctgcccatgt cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct
     6001 gctaggatca atgtggaatt caccatgtct agactggaca agagcaaagt cataaactct
     6061 gctctggaat tactcaatga agtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     6121 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     6181 gatgccctgg caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     6241 gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca
     6301 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     6361 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     6421 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggatca ggagcatcaa
     6481 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     6541 gcaattgagc tgttcgacca tcagggagcc gaacctgcct tccttttcgg cctggaacta
     6601 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt
     6661 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     6721 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     6781 catcgatacc gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
     6841 attacgcgcg cagcggccga ccatggccca acttgtttat tgcagcttat aatggttaca
     6901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctccgga ccacgtgcgg
     7021 accgagcggc cgcaagcttg tttaaacgtt aacggatcct ttgttacttt atagaagaaa
     7081 ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt
     7141 tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata
     7201 aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac
     7261 gtacgtcaca atatgattat ctttctaggg ttaaagagct ccagcttttg ttccctttag
     7321 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7381 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7441 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7501 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7561 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7621 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7681 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8161 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8221 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8281 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8341 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8401 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8461 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8521 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8581 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8641 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8701 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8761 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8821 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8881 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8941 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9001 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9061 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9121 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9181 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9241 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9301 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9361 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9481 acatttcccc gaaaagtgcc ac
//
