LOCUS       Exported                8732 bp ds-DNA     circular SYN 15-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-RAM-d2TTA-TRE-FLEX-Lifeact-mScarlet-HA-EFS-WPRE-pA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Link Hyrule
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(807..826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2109..2128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     2974..2987
                     /label=Primer 2
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   3922..4051
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(3944..3955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3994..4005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4065..4379
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4073..4091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4109..4127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4144..4162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4180..4198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4216..4234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4251..4269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4287..4305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4317..4326
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4352..4376
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4404..4437
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    4488..4521
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     4522..4592
                     /label=R-HA-AscI-XhoI-mScarlet
     CDS             complement(4533..4559)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4558..5255)
                     /codon_start=1
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     4560..4587
                     /label=Primer 2
     primer_bind     complement(4637..4657)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4835..4854)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5058..5072
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5104..5122
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(5277..5327)
                     /codon_start=1
                     /label=Lifeact
                     /translation="MGVADLIKKFESISKEE"
     primer_bind     complement(5287..5340)
                     /label=Primer 1
     primer_bind     complement(5287..5327)
                     /label=F-AvrII-mScarlet
     primer_bind     complement(5307..5327)
                     /label=JDW 466
     primer_bind     complement(5323..5338)
                     /label=JDW 415
     protein_bind    complement(5340..5373)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(5424..5457)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    5502..6090
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5555..5575)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5973..5984)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6094..6105)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6123..6140)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6129..6240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6388..6436
                     /note="synthetic polyadenylation signal"
     misc_feature    6450..6541
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6490..6509
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     6556..6596
                     /label=F-MluI-EFS
     misc_feature    6562..6793
                     /label=EFS
     promoter        6581..6792
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(6760..6800)
                     /label=R-BamHI-EFS
     CDS             6937..7683
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7696..7712)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7697..7713)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7737..7756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7738..7756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7782..7916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7819..7838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7873..7892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7946..8086
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7946..8075
                     /label=AAV2 ITR
     primer_bind     complement(7992..8003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8053..8064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8223..8357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8260..8279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8314..8333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8386..8551
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca
     3901 gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg
     3961 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
     4021 ggagtggcca actccatcac taggggttcc tgcggccgca tctcgagttt actccctatc
     4081 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     4141 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     4201 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     4261 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     4321 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcca
     4381 ccggctctag agtcgactcc ggaataactt cgtataggat actttatacg aagttatgca
     4441 gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata
     4501 gcatacatta tacgaagtta tggcgcgcct taagcgtaat ctggaacgtc atatggatac
     4561 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc
     4621 accacggtgt agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg
     4681 ccgggcatct gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg
     4741 ccgccgtcct tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg
     4801 tacaaccgct cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca
     4861 ggagggaagt tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag
     4921 gtgtcctggg tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg
     4981 aagccctcgg ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag
     5041 gccctggagc cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca
     5101 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
     5161 ccctcgatct cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg
     5221 aactccttga tcactgcctc gcccttgctc accatggtgg cgaccggtgg atccccctcc
     5281 tccttgctga tgctctcgaa cttcttgatc aggtcggcca cgcccatggt ggcgctagca
     5341 taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa ttatcactgt
     5401 tattctttag aatggtgcaa agaataactt cgtataatgt atgctatacg aagttatggc
     5461 gccagcgatc caccggatct agataactga tccttatcga taatcaacct ctggattaca
     5521 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5581 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5641 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5701 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5761 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5821 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5881 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5941 ttctgcgcgg gacgcccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6001 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6061 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgactagag ctcgctgatc
     6121 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6181 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6241 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6301 ggaggattgg gaagacaata gcagactagt acgtctctca aggataagta agtaatatta
     6361 aggtacggga ggtattggac aggccgcaat aaaatatctt tattttcatt acatctgtgt
     6421 gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa
     6481 caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc
     6541 tggcctaact ggccgacgcg tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     6601 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     6661 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     6721 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     6781 gccagaacac aggggatcca gctcatgagc catggcttcc cgccggaggt ggaggagcag
     6841 gatgatggca cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca
     6901 gcctgtgctt ctgctaggat caatgtggaa ttcaccatgt ctagactgga caagagcaaa
     6961 gtcataaact ctgctctgga attactcaat gaagtcggta tcgaaggcct gacgacaagg
     7021 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     7081 cgggccctgc tcgatgccct ggcaatcgag atgctggaca ggcatcatac ccacttctgc
     7141 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt
     7201 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     7261 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     7321 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggat
     7381 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     7441 cttctgagac aagcaattga gctgttcgac catcagggag ccgaacctgc cttccttttc
     7501 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     7561 gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     7621 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     7681 taactaagta agcatcgata ccgtcgacct cgaggggggg cccggtaccc aattcgccct
     7741 atagtgagtc gtattacgcg cgcagcggcc gaccatggcc caacttgttt attgcagctt
     7801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctccg
     7921 gaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     7981 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     8041 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggaagc tgtaagcttg
     8101 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     8161 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     8221 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8341 cttatcatgt ctggatctga tcactgatat cgcctaggag atccgaacca gataagtgaa
     8401 atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc
     8461 agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc
     8521 tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt
     8581 taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg
     8641 tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata
     8701 tcaacgctta tttgcagcct gaatggcgaa tg
//
