LOCUS       Exported                9998 bp ds-DNA     circular SYN 13-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     545..568
                     /label=oJDW XXX (pBSK Ori FWD)
     primer_bind     600..616
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(667..1203)
                     /label=Tol2 3' arm / right
                     /label=Tol2 3' ARM
     misc_feature    667..868
                     /label=mini Tol2 ARM 3'
     misc_feature    complement(667..683)
                     /label=3' Tol2 ITR
     primer_bind     complement(702..735)
                     /label=oJDW XXX (Tol2 3' arm, left edge REV)
     primer_bind     1121..1146
                     /label=oJDW 109 (PPT2KXIGC TOL2-3' FOR SEQ)
     primer_bind     1159..1182
                     /label=oJDW 1145 (Tol2 3' arm 5' end REV)
     primer_bind     1190..1253
                     /label=oJDW 1630
     misc_feature    complement(1190..1203)
                     /label=Tol2 3' End
     primer_bind     1225..1253
                     /label=oJDW 1597 ccdb attR4 Fwd Cold Fusion
     primer_bind     1225..1253
                     /label=oJDW 2088
     primer_bind     1225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1226..1253
                     /label=oJDW 1372 attR4 + SpeI
     protein_bind    1244..1368
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1409..1714)
                     /label=ccdb
     CDS             complement(2056..2715)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2769..2799)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2825..2948)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2924..2954)
                     /label=oJDW 2089
     misc_feature    complement(2956..2972)
                     /label=M13 For -20
     primer_bind     complement(2963..3025)
                     /label=oJDW 1631
     primer_bind     complement(2963..2990)
                     /label=oJDW 1598 ccdb  attR3 Cold Fusion
     primer_bind     complement(2963..2990)
                     
     polyA_signal    2988..3073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3033..3059
                     /label=oJDW 161
     misc_feature    3084..3326
                     /label=cHS4 b globin insulator core
     misc_feature    3334..3576
                     /label=cHS4 b globin insulator core
     polyA_signal    3597..3731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3638..3664
                     /label=oJDW 161
     primer_bind     3732..3820
                     /label=oJDW 2065
     CDS             complement(3753..3773)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3774..3788)
                     /codon_start=1
                     /product="Gly Gly Ala Gly Gly Flexible Linker"
                     /label=G2AG2 Linker
                     /label=3X Flag GlyGlyAlaGlyGly
                     /note="Sabourin M et al., A flexible protein linker 
                     improves the function of epitope-tagged proteins in 
                     Saccharomyces cerevisiae (2007). Yeast."
                     /translation="GGAGG"
     primer_bind     3788..3820
                     /label=Primer 1
     CDS             complement(3798..4496)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     3798..3820
                     /label=oJDW 1225 (REV-mTagBFP2-1)
     primer_bind     complement(3860..3882)
                     /label=oJDW 1672
     primer_bind     4363..4384
                     /label=oJDW 1226 (REV-mTagBFP2-2)
     primer_bind     complement(4448..4471)
                     /label=oJDW 1223 (F-mTagBFP2-1)
     CDS             complement(4530..5321)
                     /codon_start=1
                     /label=zCre_C_term
                     /translation="ARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVMRRIRKEN
                     VDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAEIARIRVK
                     DISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPNNYLFCRV
                     RKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMAR
                     AGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD"
     primer_bind     4568..4590
                     /label=oJDW 1102 (R-SEQ-Cre)
     primer_bind     5320..5346
                     /label=intron REV
     intron          5322..5454
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(5430..5460)
                     /label=intron FWD
     CDS             complement(5455..5715)
                     /codon_start=1
                     /label=zCre_N_term
                     /translation="MVPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQ"
     primer_bind     5669..5696
                     /label=oJDW XXX (zCre NLS REV)
     CDS             complement(5689..5709)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5695..5737)
                     /label=Cloning REV
     primer_bind     complement(5695..5737)
                     /label=oJDW 2064
     primer_bind     complement(5695..5715)
                     /label=oJDW 1100 (F-SEQ-CRE)
     regulatory      5712..5721
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(5738..7256)
                     /label=Hsp70 promoter
     primer_bind     5891..5921
                     /label=oJDW XXX (Hsp70 REV1 )
     primer_bind     complement(5969..5989)
                     /label=oJDW 1332 (hsp70_Cre_FWD(2))
     primer_bind     complement(5969..5989)
                     /label=oJDW 1332
     primer_bind     6259..6281
                     /label=oJDW XXX (Hsp70 REV2)
     primer_bind     6898..6925
                     /label=oJDW XXX (Hsp70 REV 3)
     misc_feature    complement(7269..7785)
                     /label=Tol2 5' arm / left
     misc_feature    7529..7737
                     /label=Mini Tol2 5' ARM
     primer_bind     complement(7533..7559)
                     /label=oJDW 160 (Mini Tol2 5' arm FWD /REV Seq)
     misc_feature    complement(7767..7785)
                     /label=5' Tol2 ITR
     promoter        complement(7810..7830)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7849..7865)
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7873..7889
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        7897..7927
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(8251..8839)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9010..9870)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        9871..9975
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 aattaacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      721 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      781 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      841 aagaaaacta gagattcttg tttaagctat ttaatctcaa aaaacattaa atgaaatgca
      901 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      961 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     1021 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     1081 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     1141 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1201 gatcctaggg ggcccgttta aacgccatga ttacgccaag ctatcaactt tgtatagaaa
     1261 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1321 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
     1381 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
     1441 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
     1501 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     1561 ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca
     1621 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     1681 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca
     1741 aaggagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     1801 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg
     1861 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     1921 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     1981 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     2041 gccggatcct ctagattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     2101 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     2161 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     2221 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     2281 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     2341 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     2401 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     2461 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     2521 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     2581 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     2641 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     2701 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa
     2761 aatccacaca ttatacgagc cggaagcata aagtgtaaag cctgggggtg cctaatgcgg
     2821 ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     2881 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata
     2941 catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat caacttgttt
     3001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3061 tttttttcac tgcgatatca atcgggacag ccccccccca aagcccccag ggatgtaatt
     3121 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     3181 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     3241 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     3301 gatacgggga aaaagcttta ggctgagtgc acggggacag ccccccccca aagcccccag
     3361 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     3421 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     3481 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     3541 cacctggggg gatacgggga aaaagcttta ggctgatacg tagtagatgc ggccgcaact
     3601 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3661 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3721 atgtctggat cgcgatcgcg tctagattat catacctttc tcttcttttt tggaccacca
     3781 gcaccacctc ctcgagcatt aagcttgtgc cccagtttgc tagggaggtc gcagtatctg
     3841 gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt gattctttcc
     3901 agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg tttcttggat
     3961 ctatatgtgg tcttggcgtt tgcgatcaga tggctcccgc ccacgagctt cagggccatg
     4021 tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa ggcctcccag
     4081 ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc tctgatcttg
     4141 acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt cagcacgccc
     4201 ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga ctgcttgaag
     4261 aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag gaagctagta
     4321 gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat tctcatggtc
     4381 tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg atggttgtcc
     4441 acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc gctcatggtg
     4501 gatccggccc cgccggctcc gccgctagcg tctccatcct ccagcagtct caccatagct
     4561 cctgtctcag agtccaggtt tctgatgtag ttcatcacaa tgttcacatt tgtccatcct
     4621 ccagcctgca tgatctcagg gatgctcact ccagctctag ccatgtctct agcagctccc
     4681 actctagcag agtgtccaga ccaagccagg tatctctgtc cagagtcatc cttagctcca
     4741 tagatcagtc tgtgtgtagc ctcaaagatt ccctccagag cccttgtgct cagctggctt
     4801 gtagcagaag gagcagccac tccattcttt ctcactctac agaacaggta gttgttaggg
     4861 tcatcagcca ctccagacac agagatccat ctctccacca gctttgtcac tcccaggctc
     4921 agagccttct ccactccagc tgtgctcacc agtgtctttg ttcttccaat gtggatcagc
     4981 attcttcctc catctgttct gctgatgtcc ttcactctga ttctagcaat ctcagcaatt
     5041 ctcagcagtg tgttgtaagc aattcccagg aaagccaggt ttctgatgtc ctgacatctg
     5101 tcagagttct ccatcaggct tctcacctgg tcaaagtctg ttctctcaaa agccagagcc
     5161 tgcttagctc tctctccagc atccacattc tcctttctga ttcttctcat caccaggctc
     5221 acagcattgc tgtcagaagg tctaggcagt ccagaccttc tgtgcagcat gttcagctgt
     5281 cccaggtgct gctggattgt cttcacagcc agtcctctag cctgtggaga gaaaggcaaa
     5341 gtggatgtca gtaagaccaa taggtgccta tcagaaacgc aagagtcttc tctgtctcga
     5401 caagcccagt ttctattggt ctccttaaac ctgtcttgta accttgatac ttacctggag
     5461 gtacagcagg tagtctctca catcctcagg ctcagcaggg aaccactttc tgttgttcag
     5521 cttacaccaa gcagcccagc tcctgcagac agacagcagc atcttccatg tgtgctcaga
     5581 gaaagcctgt ctgtctctga acatgtccat caggttcttt ctcacctcat cagatgtagc
     5641 atccacaggc agagcaggta gattttggtg gacggttaga agattggaga ctttcctctt
     5701 cttcttgggc accatggtgg ccatatgcgg cacgcgtcag gaaaaaaaaa aacaattaga
     5761 attaatttta tatttataca atttatcggt gcaattgttt cattatgaaa ggatttttct
     5821 agtcaacaag tgaaattctc ttaataacca agttattatt agttgtacaa gtaaaacgct
     5881 aattttcttt tgtagacaac attaacacaa attcatttag gacatttgct agtatttatg
     5941 gttattatgc tcatataaaa taatgtcagc aacataaagt aaatgcccgt ctgttcattg
     6001 ctttttttaa aatccttatt gtgaaaacgg gaaatataat tttctagtag acctaaaata
     6061 agatgaacat aggcagtcca catgtaaaaa gattacatat acaaagttga ttttttgaca
     6121 ttaagtacat ataatctcta tataattctg attagccaat agagtacaat atattgagac
     6181 ttaccttaca gtagattctt aaatagagcg aataaacacc tcgtcgggga aaagtcctgt
     6241 caggctgctc ttttcttctt tgtcaagtcg cttctcttcg gttgagtttc tctgcggtct
     6301 ctctttcgcc ctttaaatac agcagagcgc gcacccgttc agactctaca ctgttctgga
     6361 agcctccagt gaattcgacc aatcaaaaca cagcaggcag ccgccgtcac ttcttcagct
     6421 gtcattgaga ggagccgctg agaggaatgt agtggagatt tccagagttt taccgtgaaa
     6481 ttacgcgaaa cagcggtcaa aaatacaaag agatttaaaa tgtttttttt atttgagtgt
     6541 tttattgtac ttattgctga aatatgtgcg gttgtgtgat gtgttgtttt tccagttgca
     6601 atgaatgaac aaactaaaat gataaaatgt ttgaacgatt cagtaaaaaa aaatgcaata
     6661 aaaaaatctt gctgaaatgt tttacacagt agcaaaatcg ggcctgtggt gttttcagct
     6721 acatagaagg caaacctgtt ttatataagc taatagcttt ggtcagattt gtttaacgtt
     6781 tcaacaaaac aaatggatgt ttactgtact atattgattg gttaaaacca atactttatt
     6841 ctgggattgc aggtcttaac tatttttgta gtgttaaaaa caacttgcta acttgagagg
     6901 tcatgtttac cctcagagaa ataggcacaa gtctctttgt ttaatattgg agatcatcct
     6961 ccttgttcag tcgtggtctg tatttaattt taatgtatat gattgccgta aaagtgttag
     7021 aaagtttacc ctggtgctat gaaatcaatc tgctgctttt gacccagtta gtgtgtcttt
     7081 aatctaactt aatgagaccc cagtgtggtc aacaatcaat agaaacaaat gtgttgtttt
     7141 ataactgtta actcctatta ttttttatct tctgtagcat atagacaaaa catgctcgtt
     7201 ctaatagttt aataaaatta atttgatttt tggaaataac caagcgacac ccctgaacta
     7261 gtatcgatgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
     7321 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
     7381 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
     7441 gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt
     7501 tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt
     7561 ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
     7621 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     7681 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     7741 aagtacagta atcaagtaaa attactcaag tactttacac ctctgagatc tgagctccag
     7801 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     7861 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7921 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7981 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8041 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8101 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8161 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8221 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8281 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8341 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8401 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8461 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8521 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8581 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8641 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8701 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8761 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9001 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9961 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
