LOCUS       Exported               12568 bp ds-DNA     circular SYN 22-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(241..736)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="LELVDPPGCRNSISSLSIPSTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAG
                     VVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKL
                     D"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..736
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     780..796
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    957..1433
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     protein_bind    1454..1578
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1619..1924)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2266..2925)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2979..3009)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3034..3158)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3162..10326)
                     /label=B-MHC / MyH7 promoter
                     /note=" NM_001361607.1"
     exon            complement(3162..3170)
                     /label=Exon 4
     misc_feature    complement(3568..3625)
                     /label=Exon 3
     exon            complement(3853..3890)
                     /label=Exon 3
     misc_feature    complement(4598..4891)
                     /label=Exon 1
     misc_feature    complement(4776..4814)
                     /label=Guiick et al exon 1
     misc_feature    4840..4845
                     /label=TATA
     misc_feature    4871..4876
                     /label=E-box
     misc_feature    5005..5012
                     /label=BFe
     misc_feature    5049..5058
                     /label=C-rich
     misc_feature    5089..5095
                     /label=MCAT
     misc_feature    5807..5812
                     /label=E-box
     primer_bind     complement(10327..10343)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(10352..10423)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAV"
     misc_feature    10352..10367
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(10380..10398)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10419..10435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10443..10459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10467..10497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10512..10533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10821..11409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(11416..11425)
                     /label=TCF/LEF DBD
     CDS             complement(11580..12440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12441..12545)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt tctagagcgg ccgcccgggc gccacgtgta tactagttta aacggccgct
      781 ctagaactag tggatccccc gggctgcagg aattcaacag gcatctactg agtggaccca
      841 acgcatgaga ggacagtgcc aagcaagcaa ctcaaatgtc ccaccggttg ggccatggca
      901 ggtagcctat gctgtgtctg gacgtcctcc tgctggtata gttattttaa aatcagaagg
      961 acagggaagg gagcagtggt tcacgcctgt aatcccagca atttgggagg ccaaggtggg
     1021 tagatcacct gagattagga gttggagacc agcctggcca atatggtgaa accccgtctc
     1081 taccaaaaaa acaaaaatta gctgagcctg gtcatgcatg cctggaatcc caacaactcg
     1141 ggaggctgag gcaggagaat cgcttgaacc caggaggcgg agattgcagt gagccaagat
     1201 tgtgccactg cactccagct tggttcccaa tagaccccgc aggccctaca ggttgtcttc
     1261 ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata gaaggacacc
     1321 tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg cactggagtg
     1381 gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca cccatcaagc
     1441 tcgacgcgtt atcaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa
     1501 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     1561 acatatccag tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt
     1621 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
     1681 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
     1741 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
     1801 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
     1861 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
     1921 gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
     1981 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
     2041 tcattctgca tggttgtgct taccagaccg gagatattga catcatatat gccttgagca
     2101 actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt
     2161 tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa
     2221 atacgcatac tgttatctgg cttttagtaa gccggatcca cgcgtttacg ccccgccctg
     2281 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2341 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2401 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2461 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2521 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2581 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2641 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2701 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2761 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2821 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2881 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2941 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
     3001 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
     3061 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3121 ttttacgttt ctcgttcagc ttttttgtac aaacttgtaa ctggctgagc ctggggtgag
     3181 gagcagctgt tacctatatg ccttcccgcc ccttgggctc tcccactcag tggcagctcc
     3241 caaatccggt actgtgctca gcactcagga gtttaccctt ctccaggctc acccctaaac
     3301 cagagagcag cctggcaact gtgccaaggc aagggggcag ctttactttc attcacttgg
     3361 ggaactcttt gggtgtgtcc tgaccgtctt tcggggacag aactctgtat caatctctga
     3421 ctgtcaatag tccctgcccc tcactggcca ggccattgtc tctgagcagg cagaaaggaa
     3481 cctgccaggc ttcgggcttg gggctttggg ctttaactat gtgcagtttt gccagatctc
     3541 ccaacatttg gactccaact tttctcacct tggattctca aacgtgtcta gtgatagaga
     3601 ggtaagccca ggcctgtaga agagctggag aaagggaaga gacggtgtca ggacaggctg
     3661 tggcccagcc tggcttctgg cacctccctc ctctctggct ctgttcttca gcacgcggaa
     3721 gagtgattag cagagctggt cagcctttct ggtcactggc atccttcctt ttcaagctct
     3781 atctcctttc tcccaaagac taacaacctc cgagccccag tcctccgccc ttaggggctg
     3841 ttctaccctt acctgtactc ctctgctgag gcttcctttc tcggagccac ctacaacaca
     3901 acaatagtca caatgcactc ttgctgttca ctctcacggc tgccccttgg gtacgtgagg
     3961 aagtggaggc tgaagcctta cttttctcag agcacaggac aagtagcaga gtccagattt
     4021 cagactcgag cctcatgatt ccaacagccc tctgactacc ctcgcacacc ccagcctccc
     4081 ctgcacacgc cctgataaat acacatgcct tctgcttgtg cccacacttg acgtgaatgt
     4141 aaacttcctt ccatcctcag cacacatgct ccccacagtg ttcatgtgca aacagcccag
     4201 cacataggca ggctcctgtg gctgcgtgtt cttcagcata cgaagaccag agccccccac
     4261 acctgcatgt ctgtgaactg ctatttcaga gactacacgc agggtcatat cctacatagt
     4321 aataacccct ctatgtacag tcagacactt ccccaaacac aggcaggctc tagtgttcac
     4381 agaactggct tccttaagag ttattctcca tcattctgtc cctcttctgc caaaatgctt
     4441 gtaaccaggc atgaggtacc ttatgtgata aaatataaac tgatccttac atttaagata
     4501 tgtgtgcttg tacttgcaca cgcacacatg cacgcacatg ctcacacact gctattgcat
     4561 tctgggccat atgcacatat agatatccac cccccacctt ggaacacttg ggctcccaca
     4621 ttcctacggc acaagcatgc gtatttagat ctgcacagga gtgttcaatg ataacctggt
     4681 actacatgcc agaagcccgc tgctgttcta gaggaattac ttcctgaacc tggagcccct
     4741 tatcccagag ggaagcctcc agctcccact cctacctgag ggtagcaagt aaggaaacag
     4801 caggagaatg ctcagagcca gacctggtct caggggctgt atatatgggg cagcagggtg
     4861 ccccatcccc cacctgcacc cagtccctag ccagatttag aaagggctgt tgtcactaaa
     4921 agcatttccc ccccgactct cccccccttc ccttgtccaa acagtactcc cccctcctcc
     4981 cccagctagg aaacaattgg aagtggtcgt cattgttgtg gcatggcatg ttcagggtct
     5041 gagttccagg gggtggggca gcttaaagtg aagcaaaaat atctctaaca ttccacggct
     5101 cactagagag taagctgacc acgaccacca cgacctcaag caggcaaggc cctggctggc
     5161 ctgcaagagg gtcctgctga ccctggcatt gctgccccct tagaagcctt gcccccagtg
     5221 ctagcctggc cctatttcca gtatcctccc cacactcctg gcttgagtcc tgcatctgtc
     5281 tgaggtggga aggggttggg tcacagcagc tgagaggggc agggcagagt tgggggcatg
     5341 gctctggtat ggtggttgga ttggagcaga tagaaggtag taggttcctg ttaggaccag
     5401 agagtttgga cacaggaatt ctggctcaca cactgctttc cttgggccag caggcaatgt
     5461 gtccctggga tcatggtgct tcagagccag gcagatcttt agtgatccat ttaagtgctt
     5521 tgctttaggg ggtgggggtg ggagatgctt cctctaagat tgcacagctt cagcgaacga
     5581 aaagtgggga acctcagcag ataggaagtt gatcccaatc aggggaggag ggctagggat
     5641 gctaagaagt atgccaaagc tgtttgggga ggaggaccag ttgctgatgg tcctgcttta
     5701 gagccctttc ctgttctagc gctgccctgc agattttcga ggcgcaaggc ctgagcctcc
     5761 cagggttgcc cagggctgta gatgatatgg ctacggctgc ctggtccacc tggcctctgt
     5821 ctagccgtgg gtgcaatctt tgttcttcct agtggagaga gggacaggac aggagagacc
     5881 ttctcttccc ctgcaggccc cgggagcaac gctggcaccc agcctcgttc ttgttcttcc
     5941 agcatgggct atggaagctt cactgctcgg tgtctaggca acagactcag ggtttagttt
     6001 gaaatcccct ttcttcccaa acacagctct gagcgcatgc ggattaatcc agttgattag
     6061 atgtcagttt gaaggagaga ggcaggcctg ggggatgggc tgccagcaaa gcaggtacat
     6121 tgtgaaggtg gggtaggcat ggcagggaaa ggaaaaatcc aggaagggct tctgtggggt
     6181 ttcttggtaa ccaaagcagg ggtaaggatg ctgagcagct gtgtgcagtg tgcaggggga
     6241 aggcaggggg aaggtggggg gaggaccaca gactgtaggg gctgcacctc aatgcagctg
     6301 cagctccgca gtcctcagaa tgcagttgca gttgctgaag aactggtctt gcgaccctgc
     6361 caggtattaa tagagcagcc tccctaggag gaacctccag ggccggagga acctccaggg
     6421 ccgaaggaag gctaaagctc tcccaggctc ttcttttttt gggggggggg tggtttgaga
     6481 cagggtttct ctgtatagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct
     6541 cgaacttaga aatccacctg cctctgcctc ccaagtgctg ggattaaagg cgtaccacca
     6601 ggctctccca ggctcttcta gctttcccaa cagaagaagg tgccttcccg ccatgctctg
     6661 ggagagggct caggcacagg tgcacgcact gagatggata aagaaagctt gggactcagg
     6721 cgagccattt taaagccagt gtctttgagt ggggccaacc tgtgtggctg tgggattagg
     6781 tagtcacctc tacaatttag ccacacaccc tttccccctt ctttcaggtt tggagggctt
     6841 tggtctaaag gtgtttttgg agtattgttc tcaactctgc aaggcagaat ttaaaagcca
     6901 gtttcctaat tttaagatct ctgtctcata tcccagatgt ttggcctcct gcttgctgct
     6961 gttggcggtt gggtggctct tcccatcctt tcctatgtga gtattcattg agggccaact
     7021 ttgagcttgg cactgatgtg gtcaatggag atacgtaaca atgaaccaaa gcaaagaccc
     7081 ctgctccggg agctttgtcc agctgattgt agcagtggac ccaggcttca gagcacagct
     7141 tgtgctttgc atgcgtgcgt gacattccta gtggagagaa tgggggcccg aacccatgtg
     7201 aggagtgggt ggtgatgtgg agcacactac agagtgacag gagacaagac aaaggggcag
     7261 caagtgctat aaataaaaag ggaggctgct ttgctgaggt cgaccaaata aggcctggac
     7321 agaaggaagg tgatctgcct cccgagcctc ccctccccag gcattgtgcc tgctttgtct
     7381 gggctctgtc accacactga tcaaatgtca ggtccaaata ggagccattt gctgtgaaca
     7441 tgccagtgga gaccagcgga aagtttgcta aaattactgc tgggaattag ggggaggtgg
     7501 gggtctgcag tcttgaacta aaggagaact gagcatggaa agagaattct gaaaggaatc
     7561 tcaaaaccca aagggagagg ctcaggagga ggcagaagag gtagggcgtc tggcccaagg
     7621 ggaggaggag gccttagttg tctgagaaca ggagagggga cccaggagtg gtagccagca
     7681 cttgccccta gctgcctgta tcccctgacc ttggagaacc tatccctggc tccacatggg
     7741 ccaggattag ggagctgagc tagtctctct caggtttctc tttacaagca gctgctcctt
     7801 cttgtcattc ttttgtactc cgctggttcc aaaatgcttt gttctctgca tactcaaaac
     7861 accgaccact caatacacca ggcaccccaa tctcgacttt ctgatagaca gggaccagct
     7921 gggtaggcag accagtttca ataagagtct attaggcagg tagatctctc cttcaagagt
     7981 gtttggagag ggcactgtgt ggagtggcca gtctagaaac ttgagggagc ttaaaggggg
     8041 gctaccgggg agcacagaga tcccctttga gaccttgctc cacagtttct agctgtatgg
     8101 tcagtcacat aactccctga gacagttttg ccatcaatgt ggttttttgc aaggattatg
     8161 gagactaaac acaggtgagg gacctagcac acatcagcac ataacagact tcataatggt
     8221 ttttatctcc aggaacacca acaccaggga cccagactgc agacaccaga gttagttttt
     8281 ttggccccta gcatttccct cagcccacca agtctcattg gttgaagaca agaccttcac
     8341 gtgatcctag acctaaccca tctccacagg atagccttga gtcaggagac cactgccctg
     8401 ctgctaccta taaaactttt ctcacgacca tggtgaaccc caagcccacc aaaagctggc
     8461 ccagcaaaat ggcaacattg tcaggaaaac aaatggaagt ggaggggaag tgaagagtta
     8521 gctaaatgct ttttgtgtct ggggaccaca cagaaagctc ctggtagaga tggggagcca
     8581 ggttataagg aggagacagg ttaaactgtg gccctcagga ggtggtccgg ccagggaccc
     8641 ttcagatatt tctgatctta gggtcagcca aggatgcaga gggtctggaa acctctgaag
     8701 gagaatgcta gagtattctg atatggaaaa aaaatgggca aacttagctt ggtataggtc
     8761 tttaattcca gcactcagga gacagaagca tgcagatctc tgagtttgag gctagcctgg
     8821 tctatacaga gaatgccagg tcagtcaggg agacaaagta agaccctgcc agtgagagag
     8881 agtctcactg tgggactagc ctgttaaggc acccggcttt aaggaccaag tagctaccat
     8941 aatctcagct cttggctgtg attctgatgc tgttactatc ttcaagctga cttagacaat
     9001 tctttagtat atgtcttagg gtttctactg ttgtgataaa acaccatgat cagaagcaac
     9061 ttgggaggaa agggtttact tcagcccaca actctcaggt catcctctga cactgaggga
     9121 agccaggaca agaactcaag gtaagagtct ggtggaggca ggggaactaa gtctgcttac
     9181 tggcttgctc cccaatggct tgcccagtct gattcttgta acacccaagc actcttgctt
     9241 aggggtggca ccacctgcaa tgggctgtgc tgtcctgtat caataattaa ttaaaaagcc
     9301 aatctgatgg ggacattttt tttcagttga ggttcctctt cctcctcata tgatcctagc
     9361 tgtgttgagc gaacaacaac aaaactaacc aggacagtat gcatattctt aaaagtgggc
     9421 aatctggaga agagcaaagg atggggttag agagatggct cactgtttag agcacttgct
     9481 gttcttgcag gggaatggag gtctgattct cacaacccac agggcagcat acaaccttat
     9541 gtaattccag ttccgagaga tcctgttgca tgctggcctg cggcttacaa ggaccgggta
     9601 caccagggag gcaggctggg gctgaaagag acttagatgg caagacatga ttctgctcaa
     9661 ggctctggcc tccaggtgtg ggggagctgg cttttagaat tagcaaaaag gccagagatt
     9721 ctctggaagt ggaggctgtg ctctgaatta actttgctag gtaattaaga tttttagcta
     9781 tcttcaattg gaatctctaa tattggactt attactagac cagtccaaga ttgagttaga
     9841 ataactggtc acattgaagg aaaaaggaga atcattataa ttctttctta tgactaatct
     9901 ttctaagtca gattccacag tctttgatgt tctttttacc ataaggttaa aaagcttgaa
     9961 acaaggcagc tttgtttgtc caaactggaa aaatggcaag caaggatggg tcaaaaacat
    10021 gtacccaatt cagcttcctt gtcactacag tcactacagt cagggcactg agcgaactca
    10081 cctgtcttgt cacaggaatt agccatcatg cttctgctcc gttctgtgga gagaacctat
    10141 gcttttccct ttcctaataa ggcagctgtt caggatcaac catatgccaa taagtagatc
    10201 cttttaggat actataaagc atttattaag ttctttgaca tccaaatttc aaaattctaa
    10261 aataaaagta tgatttaaat aatgtgcatg ggaatgcact aaataacatg cacggggata
    10321 taattggatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
    10381 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
    10441 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10501 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10561 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10621 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10681 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10741 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10801 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11221 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    11281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11461 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11521 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11581 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11641 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11701 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11761 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11821 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11881 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11941 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12001 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12061 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12121 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12181 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12241 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12301 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12361 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12421 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12481 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12541 ccgcgcacat ttccccgaaa agtgccac
//
