LOCUS       Exported               12023 bp ds-DNA     circular SYN 18-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(707..941)
                     /gene="piggyBac 3' inverted terminal repeat"
                     /label=piggyBac Transposase binding site
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 3'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 3' ITR and flanking 
                     sequence."
     misc_feature    complement(711..773)
                     /label=PiggyBac 3' ITR
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1431..1536
                     /label=CMV minimal pro
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1573..1619
                     /label=oJDW 1368
     regulatory      1585..1594
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1591..3240
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1592..1614)
                     /label=oJDW 406
     primer_bind     complement(1641..1661)
                     /label=oJDW 1244 (Luciferase N-term REV)
     primer_bind     complement(2417..2444)
                     /label=oJDW 1405
     primer_bind     complement(3184..3215)
                     /label=oJDW 1406
     primer_bind     complement(3215..3248)
                     /label=oJDW 1369
     CDS             3250..3306
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3282..3332
                     /label=oJDW 1017 (P2A_AvrII_H2A_FWD)
     primer_bind     3282..3332
                     /label=oJDW 1017
     regulatory      3308..3315
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3313..3696
                     /codon_start=1
                     /label=H2A.F/Z
                     /translation="MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKTRTTSHG
                     RVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKAT
                     IAGGGVIPHIHKSLIGKKGQQKTA"
     primer_bind     3313..3332
                     /label=oJDW 1370
     CDS             3703..4413
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             3703..4410
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4389..4410)
                     /label=oJDW 1371
     primer_bind     complement(4391..4451)
                     /label=oJDW 1018
     protein_bind    complement(4431..4455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4568..4585)
                     /label=oJDW 748
     primer_bind     complement(4570..4589)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4635..4690
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4689..4708)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     5025..5078
                     /label=oJDW 1403 pB-TetON-FWD ColdFusion
     primer_bind     5057..5084
                     /label=oJDW 1849 rtTA FWD + AscI
     misc_feature    5071..5162
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5111..5130
                     /label=oJDW 407
     primer_bind     5163..5196
                     /label=oJDW 1845 Human EF-1a FWD + AsiSI
     promoter        5200..6378
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5431..6369
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6369..6399)
                     /label=oJDW 1846 Human EF-1a REV + SpeI
     misc_feature    6404..6412
                     /label=kozak
     CDS             6416..6535
                     /codon_start=1
                     /gene="Mus musculus ornithine decarboxylase gene 
                     destablization sequence.
                     
                     http://www.jbc.org/content/278/14/12135.abstract
                     "
                     /product="COOH 37 amino acids of mODC"
                     /label=mODC DEST
                     /note="mm Odc1 destablization domain"
                     /translation="SHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     CDS             6545..7291
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7292..7337)
                     /label=oJDW 1404 pB-TetON-REV ColdFusion
     misc_feature    7318..7762
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     primer_bind     complement(7753..7781)
                     /label=oJDW 1863 hEF1a rtTA REV + b-Globin polyA + AscI
     primer_bind     complement(7753..7781)
                     /label=oJDW 1850 rtTA Rev + b-globin polyA + XhoI
     promoter        7794..7841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7795..7841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7833..7851
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7861..8655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7915..7934)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8525..8544
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8702..9274
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     protein_bind    9374..9686
                     /gene="piggyBac 5' inverted terminal repeat"
                     /label=piggyBac Transposase binding site
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 5'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 5' ITR and flanking 
                     sequence. May contain cryptic promoter activity."
     repeat_region   9648..9682
                     /label=piggyBac 5' ITR
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9832..9850)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9871..9887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9871..9887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9884..9906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9895..9911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9919..9949)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9964..9985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10102..10119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10273..10861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10353..10372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11032..11892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11655..11674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11893..11997)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggatccac tagtgccacc atggaagacg ccaaaaacat aaagaaaggc
     1621 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     1681 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     1741 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     1801 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     1861 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     1921 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     1981 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     2041 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     2101 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     2161 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     2221 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     2281 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     2341 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     2401 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     2461 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     2521 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     2581 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     2641 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     2701 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
     2761 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     2821 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     2881 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
     2941 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
     3001 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     3061 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     3121 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     3181 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
     3241 ggtaccggag ccactaactt ctccctgttg aaacaagcag gggatgtcga agagaatccc
     3301 gggccaccta ggatggcagg tggaaaagca ggtaaagaca gtggaaaagc caagacgaaa
     3361 gccgtctcca gatctcagag agctggtctg cagttccctg taggtcgtat ccacaggcac
     3421 ctgaagaccc gaaccaccag ccatggccgt gtcggagcta cagctgcggt ttacagtgct
     3481 gctatcctgg agtatctcac cgctgaggtg ctggagttgg ctggaaacgc ttctaaggat
     3541 ctgaaggtga agcgaatcac tcccagacat ttacagctgg ccatccgcgg agacgaggag
     3601 ctggactcgc ttatcaaggc caccattgct ggaggaggag tcattcctca catccacaag
     3661 tctctgattg gtaagaaggg ccagcagaaa accgcagggg ggatggtgag caagggcgag
     3721 gaggacaaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3781 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3841 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     3901 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3961 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     4021 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     4081 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     4141 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4201 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
     4261 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gctggacatc
     4321 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
     4381 tccaccggcg gcatggacga gctgtacaag taatgatagc tcgagagatc tcccagcttt
     4441 cttgtacaaa gtggtgatgg ccgctctagc gaagggatcc cccatcgaat tcactcctca
     4501 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     4561 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     4621 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     4681 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     4741 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     4801 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     4861 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     4921 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     4981 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcaggaa ttcgattact
     5041 taagcctagg atccgtacat tcgaatcgat aacatacgct ctccatcaaa acaaaacgaa
     5101 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     5161 ctgcgatcgc ttctaggtct tgaaaggagt gcctcgtgag gctccggtgc ccgtcagtgg
     5221 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     5281 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5341 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5401 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     5461 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     5521 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     5581 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     5641 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     5701 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     5761 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     5821 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     5881 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     5941 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     6001 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     6061 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     6121 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     6181 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     6241 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     6301 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     6361 ccatttcagg tgtcgtgagg aattagcttg gtaactagtg ggagccgcca ccatgagcca
     6421 tggcttcccg ccggaggtgg aggagcagga tgatggcacg ctgcccatgt cttgtgccca
     6481 ggagagcggg atggaccgtc accctgcagc ctgtgcttct gctaggatca atgtggaatt
     6541 caccatgtct agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg
     6601 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     6661 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     6721 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     6781 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt
     6841 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     6901 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     6961 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     7021 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     7081 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     7141 acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat
     7201 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     7261 cgattttgac cttgacatgc tccccgggta actaagtaag gatcccgtac gcctaggagc
     7321 aggtttcccc aatgacacaa aacgtgcaac ttgaaactcc gcctggtctt tccaggtcta
     7381 gaggggtaac actttgtact gcgtttggct ccacgctcga tccactggcg agtgttagta
     7441 acagcactgt tgcttcgtag cggagcatga cggccgtggg aactcctcct tggtaacaag
     7501 gacccacggg gccaaaagcc acgcccacac gggcccgtca tgtgtgcaac cccagcacgg
     7561 cgactttact gcgaaaccca ctttaaagtg acattgaaac tggtacccac acactggtga
     7621 caggctaagg atgcccttca ggtaccccga ggtaacacgc gacactcggg atctgagaag
     7681 gggactgggg cttctataaa agcgctcggt ttaaaaagct tctatgcctg aataggtgac
     7741 cggaggtcgg cacctttcct ttgcaattac tgaccctatg aatacactga ctgtttgaca
     7801 attaatcatc ggcatagtat atcggcatag tataatacga ctcactatag gagggccacc
     7861 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7921 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7981 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     8041 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     8101 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     8161 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     8221 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     8281 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     8341 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     8401 ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     8461 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     8521 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8581 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8641 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg aattcgctag
     8701 gattatccct aatacctgcc accccactct taatcagtgg tggaagaacg gtctcagaac
     8761 tgtttgtttc aattggccat ttaagtttag tagtaaaaga ctggttaatg ataacaatgc
     8821 atcgtaaaac cttcagaagg aaaggagaat gttttgtgga ccactttggt tttctttttt
     8881 gcgtgtggca gttttaagtt attagttttt aaaatcagta ctttttaatg gaaacaactt
     8941 gaccaaaaat ttgtcacaga attttgagac ccattaaaaa agttaaatga gaaacctgtg
     9001 tgttcctttg gtcaacaccg agacatttag gtgaaagaca tctaattctg gttttacgaa
     9061 tctggaaact tcttgaaaat gtaattcttg agttaacact tctgggtgga gaatagggtt
     9121 gttttccccc cacataattg gaaggggaag gaatatcatt taaagctatg ggagggttgc
     9181 tttgattaca acactggaga gaaatgcagc atgttgctga ttgcctgtca ctaaaacagg
     9241 ccaaaaactg agtccttggg ttgcatagaa agctgccaaa cgctagccaa caagctcgtc
     9301 atcgctttgc agaagagcag agaggatatg ctcatcgtct aaagaactac ccattttatt
     9361 atatattagt cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt
     9421 agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga
     9481 taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg
     9541 cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag
     9601 cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt
     9661 tacgcagact atctttctag ggttaaaaaa gatttgcgct ttactcgacc taaactttaa
     9721 acacgtcata gaatcttcgt ttgacaaaaa ccacattgtg gccaagctgt gtgacgcgac
     9781 gcgcgctaaa gaatggcaaa ccaagtcgcg cgaggtaccc agcttttgtt ccctttagtg
     9841 agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9901 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9961 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10021 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10081 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10141 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10201 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10261 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10321 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10381 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10441 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10501 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10561 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10621 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10681 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10741 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10801 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10861 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10921 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10981 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11041 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11101 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11161 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11221 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11281 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11341 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11401 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11461 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11521 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11581 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11641 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11701 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11761 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11821 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11881 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11941 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12001 atttccccga aaagtgccac ctg
//
