LOCUS       Exported                4210 bp ds-DNA     circular SYN 08-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 11, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    701..709
                     /label=DLL4 intron 3 FORWARD
     misc_feature    710..1493
                     /label=DLL4 intron 3
     misc_feature    856..859
                     /label=ETS #1/#A
     misc_feature    888..901
                     /label=Elk1
     misc_feature    892..895
                     /label=ETS #2/#B includes Elk1
     misc_feature    898..913
                     /label=Mef2
     misc_feature    908..923
                     /label=E2F
     misc_feature    913..919
                     /label=RBJk binding site
     misc_feature    916..919
                     /label=ETS #3/C includes CSL/E2F
     misc_feature    975..982
                     /label=PU1.1
     misc_feature    976..979
                     /label=ETS #4/#D PU1.1
     misc_feature    977..995
                     /label=HNF4
     misc_feature    1002..1006
                     /label=ETS #5/E
     misc_feature    1014..1017
                     /label=ETS #6/F
     misc_feature    1055..1058
                     /label=ETS #7/G includes cETS1
     misc_feature    1056..1068
                     /label=c-Ets-1(p54)
     misc_feature    1088..1095
                     /label=TCF4
     misc_feature    1094..1105
                     /label=NFAT
     misc_feature    1098..1101
                     /label=ETS #8/#H includes NFAT site
     misc_feature    1166..1169
                     /label=ETS #9/#I
     misc_feature    1191..1194
                     /label=ETS #10/#J
     misc_feature    1208..1211
                     /label=ETS #11/K
     misc_feature    1215..1218
                     /label=ETS #12/L
     misc_feature    1222..1230
                     /label=LMO2
     misc_feature    1261..1273
                     /label=Sox9
     misc_feature    1300..1309
                     /label=NRF1
     misc_feature    1494..1510
                     /label=DLL4 intron 3 REVERSE
     misc_feature    1517..1558
                     /label=E1b minimal promoter
     misc_feature    1531..1538
                     /label=TATA box
     misc_feature    1545..1558
                     /label=carp B actin initiator
     intron          1565..2216
                     /label=Rabbit B-globin intron
     protein_bind    2256..2379
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2475..2491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2604..3413
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3560..4148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgaa gcttctcgag agtatctaac ttctcggcca
      721 caggggggcg acatcacaca gcgccgaaag agttaaccag ttataggcgg gggtgggggt
      781 tggggacgca ggcttggggg tgggggccag gacgcttagc ttggccggag ctgcgccccg
      841 cgctggacgc tcggattccg ctcgctgcct ggactcagag cacaattgcg tttcctgcgg
      901 gttatttttg gcgtgggaac gcggggagcg cggcggtgag aaaggccgag gctgccagcg
      961 ccgctgacgg gcctcttcct gtattttaca ccttttgcga attccgctcc tttggaaagg
     1021 gaataatggc tttgggatgt tgttctgaca cagaggaaaa ggatatttca ccagcacaac
     1081 aattctcact ttgaaaagga aaaagaaaaa ccattaccta cgtctagaac agaacccctt
     1141 gctcccagtt ctcgaaccag aaaacttccc cctttaaatt ttttcttttt ttccattttg
     1201 acctcttttc ctctttcccc tccgtatctg cctccacaac cctaggatat cttaacatcc
     1261 gtccattgta cccttttttg aatgctatca agccccctgc acatgcacac acccagggag
     1321 actaagtagc aagattctgg gaccctctgg cctgtgctta cttgcaggta gagttaatct
     1381 agataattag agtgtgaact gaccaccata gtcacaacta aagagagagt tggcagcagt
     1441 caactctctc tgaatcaggt tggctttctg aatcaggttc tctgaccaaa gcctctttct
     1501 gcgcggccgc actagttcga ctctagaggg tatataatgg atcccatcgc gtctcagcag
     1561 atctcgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg
     1621 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg
     1681 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac
     1741 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa
     1801 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta
     1861 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag
     1921 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa
     1981 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc
     2041 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg
     2101 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc
     2161 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc ctcgacgtcg
     2221 acaccggtat cgatacgcgt tacgtagcgg ccgctcaagt ttgtacaaaa aagttgaacg
     2281 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     2341 tacataatac tgtaaaacac aacatatgca gtcactatga atcaactact tagatggtat
     2401 tagtgacctg tagaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg
     2461 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2521 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2581 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     2641 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2701 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2761 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2821 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2881 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2941 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     3001 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     3061 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     3121 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     3181 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     3241 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     3301 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3361 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     3421 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     3481 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     3961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4201 ctcacatgtt
//
