LOCUS       pAAV-hSyn-FLEX(c        5897 bp DNA     circular SYN 10-NOV-2024
DEFINITION  .
ACCESSION   VB241107-3611scm
VERSION     .
KEYWORDS    VB241107-3611scm.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5897
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
                     /note="color: #946a00; direction: RIGHT"
     promoter        158..626
                     /label=SYN1
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/description=Human synapsin I promoter"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="color: #512bbd; direction: RIGHT"
     misc_feature    651..684
                     /label=Lox2272
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;can't work with other types of Lox site."
                     /note="/description=Mutated Lox site with two base 
                     substitutionsof LoxP"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=a19dd1e203df40eb846e5102df13ffb4"
                     /note="color: #d84e4e; direction: RIGHT"
     misc_feature    717..750
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=b48e25a55648467f8c98b3f68faf613f"
                     /note="color: #1f36a9; direction: RIGHT"
     CDS             complement(763..2163)
                     /label={NES-OCaMP}
                     /note="/application_notes="
                     /note="/official_designation={NES-OCaMP}"
                     /note="/uuid=dfb1be4518754d2cb9a81b8fc4996a8b"
                     /note="color: #6ddaae; direction: LEFT"
     misc_feature    complement(2164..2169)
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=ba4f837cfff042e38e7561244b24cfa6"
                     /note="color: #db6901; direction: LEFT"
     misc_feature    complement(2176..2209)
                     /label=Lox2272
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;can't work with other types of Lox site."
                     /note="/description=Mutated Lox site with two base 
                     substitutionsof LoxP"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=991e0551b2034abd9c3ad4e364260ba6"
                     /note="color: #800c0c; direction: LEFT"
     misc_feature    complement(2242..2275)
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=f4dc79ba31354e87bba6736ab88dbb23"
                     /note="color: #0c5d90; direction: LEFT"
     misc_feature    2306..2903
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="color: #f58600; direction: RIGHT"
     misc_feature    2934..3141
                     /label=BGH pA
                     /label=nonstandard type:  Regulatory_Elem
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="color: #5566f5; direction: RIGHT"
     misc_feature    complement(3149..3278)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a5d684b85c014f76a7b6d8e97945f0fb"
                     /note="color: #ef6cdf; direction: LEFT"
     CDS             4206..5066
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="color: #c54b7c; direction: RIGHT"
     rep_origin      5237..5825
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="color: #d05c0a"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgctg cagagggccc tgcgtatgag
      181 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      241 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      301 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      361 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      421 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      481 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      541 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      601 gagtcgtgtc gtgcctgaga gcgcagcaag tttgtacaaa aaagcaggct ataacttcgt
      661 ataggatact ttatacgaag ttatcctcga gtaccggtca cgcgtacgca agcttgataa
      721 cttcgtatag catacattat acgaagttat cctagggcca ccttacttcg ctgtcatcat
      781 ttgtacaaac tcttcgtagt ttacctgacc atccccatcg atgtctgcta ccctgatcat
      841 ttcatcaacc tcctcatctg ttaacttctc tccaaggtct gtcatcacgt ggcgaagctc
      901 tgctgcgccg atgtagccat tgccgtcctt atcaaacacg cggaacgctt ctctaatttc
      961 ttcttcactg tctgtgtcat tcatctttct tgccatcatc gtcaggaact cagggaagtc
     1021 gaatgtgccg tcaccgtcgg catctacttc attgatcatg tcctgcagct ctgcttctgt
     1081 ggggttctgc cccagagacc ggaacaccgt ccccagctcc ttggttgtta tcgtcccatc
     1141 cccgtccttg tcaaataggg agaaagcctc tttaaattct gcgatctgct cttcagtcag
     1201 ttggtcacgc gtagcctccc agcccatggt cttcttctgc attacggggc cgtcgggggg
     1261 gaagttggtg ccgcgcagct tcaccttgta gatgaatacg ccgtcctgca gggaggagtc
     1321 ctggttaacg tgaataatgc cgccgtcctc gaagttcatc acgcgctccc acctgaagcc
     1381 ctcggggaag gacagcttaa agtagtcggg gatgtcggct gggtgcttaa tgtaggcctt
     1441 ggagccgtat gtgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt
     1501 ggtcaccttc agcttagcgg tctgaaaggc ctcgtagggg cggccctcgc cctcgccctc
     1561 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc
     1621 cttgatgatg gccatgttat cctcctcgcc cttgctcacc agactccctc ctgtacctcc
     1681 cttgtacagc tcgtccctgc cgccggtggg gtggcggccc tcggcgcgtt cgcactgttc
     1741 cacgatggtg tagtcctcgt tgtgggacac gatgtccaac tttatgtcga cgatgtaggc
     1801 gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcgg cggcgtagtg
     1861 gccgccgtcc ttcagcctca gccccttctt gatctcgctc ttcaggacgc cgtcctcggg
     1921 gtacatccgc tcggaaacca cgggtgagct cagccgacct atagctctga ctgcgtgacc
     1981 tgccttgttc cacttacgac gggatcccca tcgatcctta tcgtcatcgt cgtacagatc
     2041 ccgacccatt tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga
     2101 acctcctcca gtcttgttaa tatcaagtcc agccaactta agagcaagct cgttctgcag
     2161 catggtggct ctagaataac ttcgtataaa gtatcctata cgaagttata ggtacctgag
     2221 ctcaacatcg atagcactag tataacttcg tataatgtat gctatacgaa gttataccca
     2281 gctttcttgt acaaagtggg aattccgata atcaacctct ggattacaaa atttgtgaaa
     2341 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2401 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2461 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2521 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2581 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2641 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2701 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     2761 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2821 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2881 tttgggccgc ctccccgcat cgggaattcc tagagctcgc tgatcagcct cgactgtgcc
     2941 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3001 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3061 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3121 gaatagcagg catgctgggg agggccgcag gaacccctag tgatggagtt ggccactccc
     3181 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3241 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     3361 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3421 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3541 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     3601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3661 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     3721 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     3781 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     3841 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3901 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3961 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4021 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4141 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4201 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4261 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4321 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4381 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4441 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4501 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4561 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4621 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4681 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4741 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4801 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4861 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4921 gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4981 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5041 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5101 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5161 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5221 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5281 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5341 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5401 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5461 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5521 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5581 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5641 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5701 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5761 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5821 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5881 acatgtcctg caggcag
//
