LOCUS       pHW1143___FRT_SA        2277 bp    DNA        circular     11-NOV-2024
DEFINITION  .
COMMENT     pHW1143, Entry vector with
            FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA in slot 4 (for SapTrap
            assembly), Kan
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     895..914
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          895..914
                     /note="sequence: gttggaacctcttacgtgcc"
                     /locus_tag="oHW579f, forward sequecing primer for DR274
                     vector (SapI)"
                     /label="oHW579f, forward sequecing primer for DR274 vector
                     (SapI)"
                     /ApEinfo_label="oHW579f, forward sequecing primer for
                     DR274 vector (SapI)"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          974..1016
                     /note="sequence:
                     GATTTttGAATTCGCTCTTCgGCGgctagcGAAGTTCCTATTC"
                     /locus_tag="oHK120f"
                     /label="oHK120f"
                     /ApEinfo_label="oHK120f"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_binding    987..993
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    995..997
                     /locus_tag="GCG overhang"
                     /label="GCG overhang"
                     /ApEinfo_label="GCG overhang"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    998..1003
                     /locus_tag="NheI site"
                     /label="NheI site"
                     /ApEinfo_label="NheI site"
                     /ApEinfo_fwdcolor="#521b92"
                     /ApEinfo_revcolor="#521b92"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1004..1037
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#935100"
                     /ApEinfo_revcolor="#935100"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1004..1385
                     /locus_tag="FRT-SA-3Stop-NheI-Sl2-AAAA"
                     /label="FRT-SA-3Stop-NheI-Sl2-AAAA"
                     /ApEinfo_label="FRT-SA-3Stop-NheI-Sl2-AAAA"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1038..1062
                     /locus_tag="splice acceptor"
                     /label="splice acceptor"
                     /ApEinfo_label="splice acceptor"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1063..1113
                     /locus_tag="Flex Linker"
                     /label="Flex Linker"
                     /ApEinfo_label="Flex Linker"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1078..1101
                     /locus_tag="Flag Tag"
                     /label="Flag Tag"
                     /ApEinfo_label="Flag Tag"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1114..1124
                     /locus_tag="three frame stop codon"
                     /label="three frame stop codon"
                     /ApEinfo_label="three frame stop codon"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1125..1127
                     /locus_tag="additonal TAA for the last TGA in the3-frame
                     stop"
                     /label="additonal TAA for the last TGA in the3-frame stop"
                     /ApEinfo_label="additonal TAA for the last TGA in
                     the3-frame stop"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1128..1133
                     /locus_tag="NheI site(1)"
                     /label="NheI site(1)"
                     /ApEinfo_label="NheI site(1)"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1134..1385
                     /locus_tag="gpd sl2"
                     /label="gpd sl2"
                     /ApEinfo_label="gpd sl2"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          complement(1235..1254)
                     /note="sequence: cctttattgagaagagacca"
                     /locus_tag="oHW578r reverse sequencing for anything
                     upstream of SL2"
                     /label="oHW578r reverse sequencing for anything upstream
                     of SL2"
                     /ApEinfo_label="oHW578r reverse sequencing for anything
                     upstream of SL2"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1352..1385
                     /locus_tag="endSL2-AAAA-SapIInsertionSite-XmaI-FRT3-pMLS25
                     7"
                     /label="endSL2-AAAA-SapIInsertionSite-XmaI-FRT3-pMLS257"
                     /ApEinfo_label="endSL2-AAAA-SapIInsertionSite-XmaI-FRT3-pM
                     LS257"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          complement(1361..1408)
                     /note="sequence:
                     CGATCCAAGCTTGCTCTTCtCATTTTTgatgcgttgaagcagtttccc"
                     /locus_tag="oHK121r"
                     /label="oHK121r"
                     /ApEinfo_label="oHK121r"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1386..1388
                     /locus_tag="ATG overhang"
                     /label="ATG overhang"
                     /ApEinfo_label="ATG overhang"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1390..1396
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          complement(1484..1503)
                     /note="sequence: AGTTACGCTGGAGTCTGAGG"
                     /locus_tag="oHW580r reverse primer for DR274"
                     /label="oHW580r reverse primer for DR274"
                     /ApEinfo_label="oHW580r reverse primer for DR274"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(1571..1588)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      complement(1628..2215)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(1..816)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 TTAGAAAAAC TCATCGAGCA TCAAGTGAAA CTGCAATTTA TTCATATCAG GATTATCAAT
       61 ACCATATTTT TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA
      121 TAGGATGGCA AGATCCTGGT ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC
      181 TATTAATTTC CCCTCGTCAA AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC
      241 TGAATCCGGT GAGAATGGCA AGAGTTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA
      301 GCCATTACGC TCGTCATCAA AATCACTCGC ACCAACCAAA CCGTTATTCA TTCGTGATTG
      361 CGCCTGAGCG AGACGAAATA CGCGATCGCC GTTAAAAGGA CAATTACAAA CAGGAATCGA
      421 ATGCAACCGG CGCAGGAACA CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA
      481 TTCTTCTAAT ACCTGGAATG CTGTTTTCCC TGGGATCGCA GTGGTGAGTA ACCATGCATC
      541 ATCAGGAGTA CGGATAAAAT GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT
      601 TAGCCTGACC ATCTCATCTG TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA
      661 CAACTCTGGC GCATCGGGCT TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC
      721 ATTATCGCGA GCCCATTTAT ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG
      781 CTTCAAGCAA GACGTTTCCC GTTGAATATG GCTCATTTTA GCTTCCTTAG CTCCTGAAAA
      841 TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAgttgga
      901 acctcttacg tgccGATCAA GTCAAAAGCC TCCGGTCGGA GGCTTTTGAC TTTCTGCTAT
      961 GGAGGTCAGG TATGATTTtt GAATTCGCTC TTCgGCGgct agcGAAGTTC CTATTCtcta
     1021 gaaaGTATAG GAACTTCtaa ctaatctgat ttaaattttc agGGTACCGG CGGAAGCGAC
     1081 TACAAGGACG ACGACGACAA GGAGCCAACC ACTTGACTAA GTGATAAgct agctaagctg
     1141 tctcatccta ctttcaccta gttaactgct tgtcttaaaa tctatgcttc tctttagtat
     1201 ctaaaatttt cctagaagct tacaagtata taaatggtct cttctcaata aaggttgtat
     1261 atttattcat cttattgaat ctgccatttc ctcgtttttg cgagtttata taccttccaa
     1321 ttttctttct attgtatttt caacttctaa ttttaattca gggaaactgc ttcaacgcat
     1381 cAAAAATGaG AAGAGCAAGC TTGGATCGAC GAGAGCAGCG CGACTGGATC TGTCGCCCGT
     1441 CTCAAACGCA ACCCTCCGGC GGTCGCATAT CATTCAGGAC GAGCCTCAGA CTCCAGCGTA
     1501 ACTGGACTGC AATCAACTCA CTGGCTCACC TTCCGGTCCA CGATCAGCTA GAATCAAGCT
     1561 GACTAGATAA ACTGGCCGTC GTTTTACACG GGTGGGCCTT TCTTCGGTAG AAAATCAAAG
     1621 GATCTTCttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac
     1741 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1801 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1861 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1921 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1981 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2041 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2101 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2161 ctgacttgag catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaAACGC
     2221 CAGCAACGCA GAAAGGCCCA CCCGAAGGTG AGCCAGGTGA TTACATTTAG GTCCTCA
//
