LOCUS       pHW1392___Psnt_1        8113 bp    DNA        circular     11-NOV-2024
DEFINITION  .
COMMENT     pHW1392, Negative selection marker for RMCE crosses -
            Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR, Amp
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer          2626..2677
                     /note="sequence:
                     catttatcaatcaaactttcagtcaccATGcaaagcccaactagcaaattgg"
                     /locus_tag="oHK170f"
                     /label="oHK170f"
                     /ApEinfo_label="oHK170f"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          complement(2626..2677)
                     /note="sequence:
                     ccaatttgctagttgggctttgCATggtgactgaaagtttgattgataaatg"
                     /locus_tag="oHK169r"
                     /label="oHK169r"
                     /ApEinfo_label="oHK169r"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2656..3918
                     /locus_tag="HisCl1 cDNA"
                     /label="HisCl1 cDNA"
                     /ApEinfo_label="HisCl1 cDNA"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3922..4173
                     /locus_tag="gpd sl2"
                     /label="gpd sl2"
                     /ApEinfo_label="gpd sl2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4180..4485
                     /locus_tag="GFP-C1"
                     /label="GFP-C1"
                     /ApEinfo_label="GFP-C1"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4534..4608
                     /locus_tag="GFP-C1(1)"
                     /label="GFP-C1(1)"
                     /ApEinfo_label="GFP-C1"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4660..4779
                     /locus_tag="GFP-C1(2)"
                     /label="GFP-C1(2)"
                     /ApEinfo_label="GFP-C1"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4831..5046
                     /locus_tag="GFP-C1(3)"
                     /label="GFP-C1(3)"
                     /ApEinfo_label="GFP-C1"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    5050..5379
                     /locus_tag="tbb-2 3'UTR"
                     /label="tbb-2 3'UTR"
                     /ApEinfo_label="tbb-2 3'UTR"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(5435..5455)
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(5464..5481)
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             5552..5620
                     /note="ApEinfo_revcolor: #6495ed ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      5639..5945
                     /note="ApEinfo_fwdcolor: gray50 ApEinfo_revcolor: gray50
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             6406..7065
                     /note="ApEinfo_revcolor: yellow ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      7163..7845
                     /note="ApEinfo_fwdcolor: gray50 ApEinfo_revcolor: gray50
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_binding    65..87
                     /note="ApEinfo_revcolor: #6495ed ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     93..113
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     131..150
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          189..241
                     /note="sequence:
                     gaagggctgcaggaattcgatttccttcagaagacgtgctttcctttttcttc"
                     /locus_tag="oHK168f"
                     /label="oHK168f"
                     /ApEinfo_label="oHK168f"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer          complement(189..241)
                     /note="sequence:
                     gaagaaaaaggaaagcacgtcttctgaaggaaatcgaattcctgcagcccttc"
                     /locus_tag="oHK171r"
                     /label="oHK171r"
                     /ApEinfo_label="oHK171r"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        210..2652
                     /locus_tag="Psnt-1"
                     /label="Psnt-1"
                     /ApEinfo_label="Psnt-1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
       61 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      121 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
      181 ccgctctaga agggctgcag gaattcgatt tccttcagaa gacgtgcttt cctttttctt
      241 ctcgtatcga tcccccgtat ggaaggctcc tcgtttcaac agccaacccc ttcccattcg
      301 ttttcgtcgt ccgtcttctt cttcgtcttc ttttttcttt tcttttcctt tttctgctgg
      361 tttttgcgag aaagagtgta tgcgagaaaa gccagttggg gaaaagaaga agaagcggtg
      421 ggaggctcga cggcgcggag cacacttctt ctctgagtct gtctcttgct cacgactcca
      481 gtccaacatc tccagcttct cactaaaacg ttttcttttt tgctcgtgtg tcgtgtgctc
      541 tcaacaaacc gctaaggact ataaaatatt gaataGgtgc cagcactttt ttgttctagt
      601 tccagaaaaa gtatttcttg tattggattt tcgattgctt caccgttaca gataattttt
      661 cattgtgaaa aatatcaata tatgggggta ttttgaaaaa tgatattttc atatcgagtt
      721 ttttcaaaat cagtttggtt ttgttggata ttgttcaaaa cgcattttgc ccaaaaaata
      781 aatattgaaa ctttacaaac attatttgta gccccgttcc atgcctattc ctattttccg
      841 ttaaaagcca aaaatacaaa taaaaatgaa aaatctgttt ttcgaacaat ggtattttgg
      901 tctcatcaca cttttgactc ttctcaatta tgcttcgaaa taggaacgaa ttcaaattta
      961 actaatggca cttggggtgc atcttctttt gaaacagaaa actgaccttt cacccaaatg
     1021 aaaattacat gagcttgtga agatgacaca ggtcagtaaa aagaaaaaag tgcgtgggta
     1081 tttctttctc cataattctt gaaatttcat tggagatatt ttgaagaggt gggctcatct
     1141 aaacattaag cttaagattt ttgaaatatt tgaaagaccc aaaataactt acacaatctt
     1201 ttcaacaaaa aaaactgtca tgaattttta tttctgaaaa gtctccaaca ttgtcgacaa
     1261 aggaattgat ttttaatgtc ttcaaataat ttagaagccg acaaatcaaa actgttgatg
     1321 cccttcgctc atctctgatt tatcatcgta tttctcaatt tcccgcgaag ttcgaattta
     1381 gacatgttcc aaggtctccg aaacttttca ccccagaaca tcatacaaat attttataca
     1441 agcttgaaaa aatgttacta agaaatttta tctccagcaa cctaacatat agttactaga
     1501 aacattgttg gctgccaagt tctgagaaac actacaattt ttaaagtttt tccgaaaact
     1561 tttttaaatc tttaagtaac ttatccattc tctcagttct tgaaatatgt tagtgacaag
     1621 ttttaaatat ttttcccaag cctatcttgc aaaactcctg aattagaatc attattcaaa
     1681 gccatgggaa aatcaagaat gcaaccacca ccgcaaatgc tcgatgtgag ctttccccaa
     1741 aatttgcatc ctctacaaca cttctttttc acctgccctc cgaaagagca cctcttctct
     1801 tctttttttt gctctgacga cgacatctaa caaaaaatca attttttttc gtttttccaa
     1861 gcggtccttt cgtgtagtcg acccattcgt attatcattc gatgcttttt cacttcttat
     1921 ttttcgtcgc ttttgaattg catatagcgt gtttatcgca acgaaaaaca gacaaaaaag
     1981 aaagcaaata tgcaaaaaca aaaaaatacg aagccaataa aaaatatcta tcttcaattg
     2041 gaattcgaat tttcaattca aattcaaacc tactaaaacc tactattttc cataattata
     2101 gattctcaaa ggtctccaaa ggaatgagcc aagaacaaaa agttgaggag aatactgcac
     2161 atttcccttt ctataacttt ttcaagatct actaagattt ttgacccttg ggatcctcaa
     2221 agttttcaaa aagtaatcct gctcttctct aaatgacgtt tttttttttg ttttcatctg
     2281 ttcaaaaaac cccttaacta gtcgtcattc ttgcccttga ataataggat aatatgatta
     2341 atctaatgtc ataataatct cctcttcgtc tcccatattt tttcccatac aaggatttca
     2401 tttttacatc cgtgtttctc gtatcagaat gtattcttct tctggaaaag taaacagact
     2461 cctacatctc tcgcctcttt ctgaaaaatt ctaattattt cgcttctgta aacacatttt
     2521 catccggggt ttgcctattt tcccattcga cccccgactc cgcctcttca ttctctcacg
     2581 gaaccctttt cttcgacgct ttgcatcttg atttacctct gatttcattt atcaatcaaa
     2641 ctttcagtca ccATGCAAAG CCCAACTAGC AAATTGGTAG AATTCAGGTG CCTGATTGCG
     2701 TTGGCAATAT ATTTGCACGC GCTGGAGCAA TCGATCCAGC ACTGCCATTG TGTTCATGGT
     2761 TACAGAAATA ACACGGAGAG CGCCGAGCTG GTCTCCCACT ACGAGTCGAG TCTTTCGCTC
     2821 CCGGACATTT TGCCCATTCC CTCAAAGACG TACGACAAGA ACCGGGCTCC CAAGCTCCTC
     2881 GGCCAGCCCA CAGTAGTCTA CTTCCATGTC ACGGTCCTCT CCCTGGACTC CATTAACGAG
     2941 GAGTCTATGA CCTATGTGAC GGACATCTTC CTTGCACAAA GCTGGCGTGA TCCTCGCCTG
     3001 CGGTTGCCTG AGAACATGAG TGAGCAGTAT CGCATATTGG ATGTCGACTG GTTGCACAGC
     3061 ATTTGGCGGC CCGATTGCTT CTTTAAGAAC GCCAAAAAGG TCACCTTCCA TGAGATGAGC
     3121 ATTCCCAATC ACTATCTCTG GCTGTACCAC GACAAAACGC TGCTCTATAT GTCCAAACTC
     3181 ACGTTGGTCC TGTCGTGCGC CATGAAGTTT GAGTCCTATC CGCATGACAC GCAAATCTGC
     3241 TCCATGATGA TCGAGAGTTT ATCCCATACG GTGGAAGATT TGGTTTTCAT TTGGAACATG
     3301 ACCGACCCAC TTGTGGTTAA CACGGAGATT GAGTTGCCGC AGCTAGACAT ATCAAATAAC
     3361 TACACAACCG ACTGTACTAT AGAGTACTCA ACAGGTAACT TCACCTGCCT GGCCATTGTG
     3421 TTCAACCTGC GCCGACGCCT GGGTTACCAT TTGTTCCACA CCTACATCCC CTCGGCTCTG
     3481 ATTGTGGTCA TGTCTTGGAT ATCGTTTTGG ATAAAACCAG AAGCGATACC CGCCCGTGTA
     3541 ACTCTGGGAG TGACCTCACT GCTAACCCTG GCCACCCAGA ATACCCAGTC GCAACAATCG
     3601 CTGCCGCCGG TTTCGTATGT CAAGGCTATA GACGTCTGGA TGTCGTCCTG TTCGGTGTTT
     3661 GTATTCCTTT CTCTGATGGA ATTTGCAGTG GTCAACAATT TTATGGGACC GGTGGCCACA
     3721 AAGGCAATGA AGGGGTATTC GGACGAGAAC ATCAGTGATC TGGACGACCT AAAGCATCAT
     3781 CGGGAATCGA TTATTGAGCC CCAGTACGAC ACTTTCTGCC ATGGCCATGC CACAGCCATT
     3841 TATATAGACA AATTCTCGCG CTTTTTCTTC CCGTTTTCGT TCTTTATACT CAATATTGTC
     3901 TATTGGACAA CGTTCCTAAA Gtaagctgtc tcatcctact ttcacctagt taactgcttg
     3961 tcttaaaatc tatgcttctc tttagtatct aaaattttcc tagaagctta caagtatata
     4021 aatggtctct tctcaataaa ggttgtatat ttattcatct tattgaatct gccatttcct
     4081 cgtttttgcg agtttatata ccttccaatt ttctttctat tgtattttca acttctaatt
     4141 ttaattcagg gaaactgctt caacgcatcA AAAATGGGTA TGGTCAGTAA AGGAGAAGAA
     4201 TTGTTCACTG GAGTTGTCCC AATCCTCGTC GAGCTCGACG GAGACGTCAA CGGACACAAG
     4261 TTCTCCGTCT CCGGAGAGGG AGAGGGAGAC GCCACCTACG GAAAGCTCAC CCTCAAGTTC
     4321 ATCTGCACCA CCGGAAAGCT CCCAGTCCCA TGGCCAACCC TCGTCACCAC CTTCTGCTAC
     4381 GGAGTCCAAT GCTTCTCCCG TTACCCAGAC CACATGAAGC GTCACGACTT CTTCAAGTCC
     4441 GCCATGCCAG AGGGATACGT CCAAGAGCGT ACCATCTTCT TCAAGgtaag tttaaacata
     4501 tatatactaa ctactgatta tttaaatttt cagGACGACG GAAACTACAA GACCCGTGCC
     4561 GAGGTCAAGT TCGAGGGAGA CACCCTCGTC AACCGTATCG AGCTCAAGgt aagtttaaac
     4621 agttcggtac taactaacca tacatattta aattttcagG GAATCGACTT CAAGGAGGAC
     4681 GGAAACATCC TCGGACACAA GCTCGAGTAC AACTACAACT CCCACAACGT CTACATCATG
     4741 GCCGACAAGC AAAAGAACGG AATCAAGGTC AACTTCAAGg taagtttaaa catgatttta
     4801 ctaactaact aatctgattt aaattttcag ATCCGTCACA ACATCGAGGA CGGATCCGTC
     4861 CAACTCGCCG ACCACTACCA ACAAAACACC CCAATCGGAG ACGGACCAGT CCTCCTCCCA
     4921 GACAACCACT ACCTCTCCAC CCAATCCGCC CTCTCCAAGG ACCCAAACGA GAAGCGTGAC
     4981 CACATGGTCC TCCTCGAGTT CGTCACCGCC GCCGGAATCA CCCACGGAAT GGACGAGCTC
     5041 TACAAGACGt aaatgcaaaa tcctttcaag cattcccttc ttctctatca ctcttctttc
     5101 tttttgtcaa aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac
     5161 tttttatagt cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc
     5221 tgtctgatgt actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa
     5281 ggttgagaaa aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa
     5341 aagaggaaac tttattgtgc cgccaagaaa aaagtctcaG TAatcaagct tatcgatacc
     5401 gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5461 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5521 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5581 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5641 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5701 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5761 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5821 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5881 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5941 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6001 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6061 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6121 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6181 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6241 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6301 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6361 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6421 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6481 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6541 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6601 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6661 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6721 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6781 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6841 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6901 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6961 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7021 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7081 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7141 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7321 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7381 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7561 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7981 agcgagtcag tgagcgagga agcggcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8101 acgcaattaa tgt    
//
