LOCUS       CN2043\pAAV-3xSP10ins-eHGT_356h-minRho*-SYFP2-WPRE3-BGHpA        5031 bp    DNA     circular      3-MAY-2019
SOURCE      
  ORGANISM  
COMMENT     rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
            
            clones 2,3,4 all good
            clone 1 low yield 
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12798192|
COMMENT     VNTDBDATE|-12798192|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2043 pAAV-3xSP10ins-eHGT_356h-minRho*-SYFP2-WPRE3-BGHpA|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    2294..2434
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2526..2832
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3351..4208
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4359..5026
                     /vntifkey="33"
                     /label=pUC\ori
     primer          complement(2176..2195)
                     /vntifkey="27"
                     /label=BGH-R
     misc_feature    2062..2265
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1803..2048
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1803..1962
                     /vntifkey="21"
                     /label=gamma
     misc_feature    1968..2048
                     /vntifkey="21"
                     /label=alpha
     misc_feature    complement(1916..1935)
                     /vntifkey="21"
                     /label=WPRE3-R\(JT)
     misc_feature    1049..1768
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    163..312
                     /vntifkey="21"
                     /label=3xSP10\core\insulator
     misc_feature    889..1008
                     /vntifkey="21"
                     /label=Rho\basal\promoter
     misc_feature    924..928
                     /vntifkey="21"
                     /label=TATA
     misc_feature    319..882
                     /vntifkey="21"
                     /label=eHGT_356h
BASE COUNT     1178 a      1370 c      1346 g      1137 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgccggta ccgaagctac ccctaacaca 
      181 ctattctaca cacagaaaat gctcttcact aggaagctac ccctaacaca ctattctaca 
      241 cacagaaaat gctcttcact aggaagctac ccctaacaca ctattctaca cacagaaaat 
      301 gctcttcact agacgcgtag gtggagtgaa gactgagagg ggtggtaggg ggagatgagg 
      361 ccgggaggag gagagtacac cctcagggat ggagagccaa caaagattct aaacgaggag 
      421 ttctgcagtg agactggcat tttggaacaa tcgtcctggt tttggttgag agcacaactt 
      481 agagaacaga ggctagaggt ggcaggctgg agtgacagcc tactgcagct gtccagccaa 
      541 ttaagccctg taggaagaat ggctgatgcc gcagtctatg tgatgctgaa ctgcggtagc 
      601 caggctgacc tggccccaat cccgggctgt gactgacctt cagatgcagg cagcagagag 
      661 aagaggacaa aagtgcaccg ctggggaaag gcaagcagct ggcacccagg gcaccaaaga 
      721 cccgcacagt gagcatcaag gccagcaagg gcacaaagcc agctgtcacc tgtcctctgg 
      781 gccaagccaa ggggggctgg cctcctggcc acctccttgg gggagcagta cccccattgt 
      841 catgacttgt tcacagattc ccagacctcc cagtgctatc aagagctcga ttcagccggg 
      901 agcttaggga ggggaggtca cttcataagg gcttgggggg ggagttggag ccacgagtcg 
      961 tccagccgga gccccgtgtg gctgtgctcc ggcctcagaa gcatccccgg atccagatct 
     1021 ttcgaagcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 
     1081 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 
     1141 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac 
     1201 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcgtgcagtg 
     1261 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 
     1321 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 
     1381 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 
     1441 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 
     1501 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa 
     1561 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 
     1621 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga 
     1681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 
     1741 tctcggcatg gacgagctgt acaagtaagt cgacggcgcg ccgcggccgc gaattcgata 
     1801 tcataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     1861 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     1921 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga 
     1981 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     2041 ttccgtggct cgagagatct tcgactgtgc cttctagttg ccagccatct gttgtttgcc 
     2101 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 
     2161 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 
     2221 ggcaggacag caagggggag gattgggaag acaatagcag gcatgagatc tcacgtgcgg 
     2281 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 
     2341 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 
     2401 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt 
     2461 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta 
     2521 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     2581 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     2641 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     2701 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat 
     2761 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     2821 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc 
     2881 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 
     2941 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg 
     3001 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 
     3061 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 
     3121 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt 
     3181 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 
     3241 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     3301 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 
     3361 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 
     3421 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 
     3481 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 
     3541 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 
     3601 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 
     3661 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 
     3721 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 
     3781 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 
     3841 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 
     3901 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 
     3961 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 
     4021 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 
     4081 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 
     4141 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 
     4201 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 
     4261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     4321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     4381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     4441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     4501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 
     4561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     4621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     4681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     4741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     4801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     4861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     4921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     4981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t 
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